Enhancement of stability of biosurfactant produced by Candida lipolytica using industrial residue as substrate

Abstract

This work describes experimental results carried out on the fermentation of Candida lipolytica, which produced a new biosurfactant when grown on a vegetable oil refinery residue as substrate. The cell-free culture broth containing the biosurfactant formed stable emulsions with hydrophobic natural compounds. Emulsification properties of the biosurfactant were not affected by salinity; however, treatment at a higher temperature decreased the emulsification activity, indicating applications in oil recovery. The isolated biosurfactant corresponds to a yield of 4.5 g/l, and the surface tension of water was reduced from 71 to 32 mN/m. Preliminary chemical characterizations showed that the biosurfactant consisted of protein (50%), lipid (20%), and carbohydrate (8%).

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

“…After the first purification, the yield reduced to 6.80 g/L and after the second purification to 5.60 g/L. The yield of the crude biosurfactant produced by C. glabrata in this study is in agreement with values reported by other studies (Morikawa et al, 2000;Rufino et al, 2007;Yadav et al, 2016).…”

“…The biosurfactant produced by C. glabrata in the renewable agro-industrial wastes (40% W and 20% CSL) medium was stable in all different temperatures (Figure 2A), range of pH ( Figure 2B), and in different NaCl concentrations ( Figure 2C) using hydrophobic substrates (soybean, cotton and canola oils) for emulsification index values. The biosurfactant produced by Candida lipolytica (Rufino et al, 2007) and a biosurfactant produced by C. glabrata (Sarubbo et al, 2006) cultivated with industrial residue have shown similar thermal stability in the temperature range tested from the results obtained in this study. The biosurfactant produced in this work by C. glabrata have the potential application in several industrial products by maintaining its emulsifying properties proven by the maintenance of its activity after temperature exposure and high saline concentration and acid and alkaline conditions.…”

Biosurfactants from Yeasts: Characteristics, Production and Application

“…Two main types of surface-active compounds are produced by microorganisms: biosurfactants and bioemulsifiers. 9 Biosurfactants significantly reduce the air-water surface tension while bioemulsifiers do not reduce as much the surface tension but stabilize oil-in-water emulsions. During the last decades, there has been a growing interest in isolating microorganisms that produce surface active molecules with good surfactant characteristics such as low CMC and high emulsification activity, simultaneously presenting low toxicity and good biodegradability.…”

“…Although produced using the same carbon source but from a different Y. lipolytica strain, Yansan, a biosurfactant consisting of a polysaccharide-protein complex with negligible lipid content, shows a much lower protein content (15%). 9 Another Y. lipolytica derived surfactant, 20 produced with hexadecane as carbon source, was found to be also a lipid-carbohydrate-protein complex with an even lower protein content, 5% and very high lipid concentration, 75%. Liposan, a biosurfactant produced in the presence of a water-immiscible substrate by yet another Y. lipolytica strain, contained no lipid in its constitution, only carbohydrate (83%) and protein (17%) and the yeast seems not to be able to produce surfactants using glucose as carbon source.…”

Production of Compounds with Emulsifying Properties byAspergillus flavusUsing Agroindustrial Residues

“…Logo, a fim de se aumentar o uso industrial de biossurfactantes, o custo de produção deve tornar-se competitivo comparado aos agentes tensoativos sintéticos. Uma possível estratégia para alcançar este objetivo é o uso de matérias primas alternativas, tais como resíduos agroindustriais [14] resíduos de destilaria [15], derivados de petróleo [16], melaço [17,5], entre outros, que têm sido descritos como substratos importantes para a produção de biossurfactantes [18,19].…”