Enhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy

Lopes, Francisco R.; Lisboa, Bianca Cristina Garcia; Frattini, Flávia; Almeida, Fernanda Martins de; Tomaz, Marcelo Antônio; Matsumoto, Priscila Keiko; Langone, Francesco; Lora, S.; Melo, Paulo A.; Borojević, Radovan; Han, Seongho; Martínez, Ana Maria Blanco

doi:10.1111/j.1365-2990.2011.01159.x

Cited by 88 publications

(45 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, VEGFA also promotes a wide range of neuronal functions, including neuronal survival and axon guidance (Mackenzie and Ruhrberg, 2012). We found that VEGFA is an injury-induced HIF-1α target gene (Figure S1) and previous studies showed that the presence of VEGFA within acellular conduits (Hobson et al, 2000) or VEGFA gene therapy (Pereira Lopes et al, 2011) increased vascularization and enhanced nerve regeneration. In addition, we verified that the levels of VEGFA are reduced in DRG of HIF1AcKO mice compared to controls (Figure S5).…”

Section: Resultssupporting

confidence: 75%

Activating Injury-Responsive Genes with Hypoxia Enhances Axon Regeneration through Neuronal HIF-1α

Cho

Shin

Ewan

et al. 2015

Neuron

125

112

View full text Add to dashboard Cite

Summary Injured peripheral neurons successfully activate a pro-regenerative transcriptional program to enable axon regeneration and functional recovery. How transcriptional regulators co-ordinate the expression of such program remains unclear. Here we show that hypoxia-inducible factor 1α (HIF-1α) controls multiple injury-induced genes in sensory neurons and contribute to the preconditioning lesion effect. Knockdown of HIF-1α in vitro or conditional knock out in vivo impairs sensory axon regeneration. The HIF-1α target gene Vascular Endothelial Growth Factor A (VEGFA) is expressed in injured neurons and contributes to stimulate axon regeneration. Induction of HIF-1α using hypoxia enhances axon regeneration in vitro and in vivo in sensory neurons. Hypoxia also stimulates motor neuron regeneration and accelerates neuromuscular junction re-innervation. This study demonstrates that HIF-1α represents a critical transcriptional regulator in regenerating neurons and suggests hypoxia as a tool to stimulate axon regeneration.

show abstract

Section: Resultssupporting

confidence: 75%

Activating Injury-Responsive Genes with Hypoxia Enhances Axon Regeneration through Neuronal HIF-1α

Cho

Shin

Ewan

et al. 2015

Neuron

125

112

View full text Add to dashboard Cite

show abstract

“…Blocking cortical ischemiainduced NSC/NPC proliferation exaggerates neuron loss in the infarct area and hippocampus and prevents spontaneous functional recovery (Li et al, 2010). It remains unclear, however, whether the NSC/NPC produced BDNF and VEGF are involved in promoting neural network rewiring in the brain after stroke as both BDNF and VEGF support neurite outgrowth (Pereira Lopes et al, 2011; Su et al, 2013), and whether NSC/NPC-induced neural network remodeling has a causal link with neurological function recovery.…”

Section: The Intrinsic Capability Of Brain Self-repair In Stroke Recomentioning

confidence: 99%

Enhancing endogenous capacity to repair a stroke-damaged brain: An evolving field for stroke research

Zhao

Willing

2018

Progress in Neurobiology

View full text Add to dashboard Cite

Stroke represents a severe medical condition that causes stroke survivors to suffer from long-term and even lifelong disability. Over the past several decades, a vast majority of stroke research targets neuroprotection in the acute phase, while little work has been done to enhance stroke recovery at the later stage. Through reviewing current understanding of brain plasticity, stroke pathology, and emerging preclinical and clinical restorative approaches, this review aims to provide new insights to advance the research field for stroke recovery. Lifelong brain plasticity offers the long-lasting possibility to repair a stroke-damaged brain. Stroke impairs the structural and functional integrity of entire brain networks; the restorative approaches containing multi-components have great potential to maximize stroke recovery by rebuilding and normalizing the stroke-disrupted entire brain networks and brain functioning. The restorative window for stroke recovery is much longer than previously thought. The optimal time for brain repair appears to be at later stage of stroke rather than the earlier stage. It is expected that these new insights will advance our understanding of stroke recovery and assist in developing the next generation of restorative approaches for enhancing brain repair after stroke.

show abstract

“…Several clinical and experimental studies justify these findings by the action of the growth factors present in PRP that may have contributed to the better axon regeneration, owing to mitogenic action on Schwann cells and their neurotrophic activity 5,[17][18][19] .…”

Section: Acta Cir Bras 2017;32(8):617-625mentioning

confidence: 99%

Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study

Roque

Pomini

Buchaim³

et al. 2017

Acta Cir. Bras.

View full text Add to dashboard Cite

Results:In the morphological analysis of all groups, myelinated nerve fibers with evident myelin sheath, neoformation of the epineurium and perineurium, organization of intraneural fascicles and blood vessels were observed. In the morphometry of the distal stump fibers, SVPRP group had the highest means regarding fiber diameter (3.63±0.42 μm), axon diameter (2.37±0.31 μm) and myelin sheath area (11.70±0.84 μm 2 ). IOVPRP group had the highest means regarding axon area (4.39±1.16 μm 2 ) and myelin sheath thickness (0.80±0.19 μm). As for values of the fiber area, IOVNF group shows highest means (15.54±0.67 μm 2 ), but are still lower than the values of the Sham group. Conclusion: The graft filled with platelet-rich plasma, with use standard (SVPRP) or inside-out vein (IOVPRP), promoted the improvement in axonal regeneration on sciatic nerve injury.

show abstract

Enhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy

Cited by 88 publications

References 38 publications

Activating Injury-Responsive Genes with Hypoxia Enhances Axon Regeneration through Neuronal HIF-1α

Activating Injury-Responsive Genes with Hypoxia Enhances Axon Regeneration through Neuronal HIF-1α

Enhancing endogenous capacity to repair a stroke-damaged brain: An evolving field for stroke research

Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study

Contact Info

Product

Resources

About