Enhancement of radiation-induced oxidative stress and cytotoxicity in tumor cells by ellagic acid

Bhosle, Sushma M.; Huilgol, Nagraj G; Mishra, Kaushala Prasad

doi:10.1016/j.cccn.2005.03.037

Cited by 123 publications

(56 citation statements)

References 43 publications

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“…41 Ionizing radiation is known to induce oxidative stress through generation of ROS which is an imbalance in prooxidant, antioxidant status in the cell. 42 In the present study, γ-irradiation caused a noticeable increase in serum level of glucose concentration with a decrease in serum insulin level. These data agree with that reported in previous study of Kafafy,43 who reported a significant increase in blood glucose concentration after exposure to whole body γ-irradiation and this might be related to endocrine abnormalities induced by irradiation that promote the secretion of biologically active peptide which has relation with carbohydrate metabolism by increasing gluconeogenesis in liver .…”

Section: Discussionsupporting

confidence: 51%

Modulatory Role of Selenium Nanoparticles and Grape Seed Extract Mixture on Oxidative Stress Biomarkers in Diabetic Irradiated Rats.

Abdelaleem¹,

Hameed²,

Askar³

et al. 2016

IJPER

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Introduction: Diabetes is a metabolic disorder of several etiologies, many oral anti-hyperglycemic agents such as sulfonylurea, biguanides are obtainable, but these agents have side effects, thus there is need of a new natural anti-hyperglycemic agent. Methods: The present study was performed to evaluate the protective role of selenium nanoparticles-grape seed extract (SeNPs-GSE) mixture in ameliorating the changes in the oxidative stress biomarkers induced by gamma radiation in diabetic rats. Experimental model of diabetic irradiated rats was induced by single intrapertoneal injection of streptozotocin (STZ 45 mg/kg) followed by γ-radiation exposure (4 Gy). Forty eight rats were randomly classified into 6 experimental groups: normal, diabetic, irradiated, diabetic irradiated, diabetic treated with either SeNPs-GSE mixtureor glimepiride(1 mg) for (14) days followed by γ-radiation. Results: Results of the present study indicated that rats exposed to γ-radiation and, or STZ significantly increase serum glucose& liver MDA levels. This increase was accompanied by a decrease in the levels of serum insulin, total antioxidant (TAC), liver enzyme activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), as well as liver tissue contents of vitamin C (Vit.C) and vitamin E (Vit.E). Diabetic rats treated with (SeNPs-GSE) mixture or glimepiride before γ-radiation exert a significant improvement in all tested biochemical parameters. Conclusion:The present study showed that a mixture of (SeNPs -GSE) possesses antioxidant and anti-diabetic activities by decreasing oxidative stress biomarkers as well as blood glucose level tested in this study. The tested combination (SeNPs-GSE) mixture is more or less equally active as that of the standard tested anti-diabetic drug glimepiride

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Section: Discussionsupporting

confidence: 51%

Modulatory Role of Selenium Nanoparticles and Grape Seed Extract Mixture on Oxidative Stress Biomarkers in Diabetic Irradiated Rats.

Abdelaleem¹,

Hameed²,

Askar³

et al. 2016

IJPER

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“…The increased production of NO in the irradiated group is due to induction of NO synthase enzyme, which is considered to be absent under physiological conditions and induced by radiation. Superoxide anion (O 2-) can attack NO to form peroxynitrite (ONOO -) anion, which may oxidize many biological molecules including sulfhydryls (such as glutathione), iron sulfur centers and lipids [33].…”

Section: Parametersmentioning

confidence: 99%

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

Km¹

2017

OMICS J Radiol

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The present study was designed to investigate the oxidative stress, which is due to the effect of low doses of gamma irradiation. Animals were divided into 6 groups, where the first group kept as control group, while 2 nd , 3 rd , 4 th , 5 th and 6 th groups were exposed to gamma ray at 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy once weekly for one month respectively. Rats were subjected to gamma radiation and nitric oxides (NO), superoxide dismutase (SOD), malondialdehyde, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and white blood cells (WBCs) were measured. SOD recorded highly significant decrease with percent change -22.7%, -25.01%, -55.01% -55.4% and -56.38% respectively in groups which were exposed to 1.5, 2, 2.5, 3 and 3.5 Gy. MDA serum level have a significant increase in groups which were exposed to successive doses of 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 25.7% , 41.8% 59.1% 65.6% 75.8% consequently as compared to control group. Nitric oxide serum level is markedly showed highly significant increase in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 59.69%, 74.17%, 87.1%, 120.1% and 130.6% consequently as compared to control group. ALT and AST recorded highly significant increase in rats exposure to successive doses of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. On the other hand WBCs recorded highly significant decreased in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. WBCs recorded percent change -26.11%, -32.3%, -47.8%, -50.1% and -53.6% respectively as compared to control group.

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“…Metabolic oxidative stress mediated by O 2 • -and H 2 O 2 significantly contributes to the differential susceptibility of cancer versus normal epithelial cells to glucosedeprivation or 2DG-induced cytotoxicity [8]. Bhosle et al [25] have documented that a decrease in mitochondrial potential is associated with cell death either by apoptosis or by necrosis. FA was also found to increase the radiosensitivity of HepG2 cells, and this was attributed to an enhanced rate of apoptosis [50].…”

Section: Discussionmentioning

confidence: 99%

“…Curcumin, a phenolic phytochemical, was shown to induce apoptosis in several human cancer cell lines but not in normal foreskin fibroblast cells [51,52]. Ellagic acid, a similar phenolic acid, enhanced the radiation-induced oxidative stress and cytotoxicity in HeLa cells [25].…”

Section: Discussionmentioning

confidence: 99%

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2-deoxy-D-glucose combined with ferulic acid enhances radiation response in non-small cell lung carcinoma cells

Reddy

Prasad

2011

Open Life Sciences

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Abstract:The present study was undertaken to investigate the radiosensitizing effects of 2-deoxy-D-glucose (2DG), a glycolytic inhibitor, and ferulic acid (FA), a phenolic prooxidant, in relatively radioresistant human non-small cell lung carcinoma cells (NCI-H460). NCI-H460 cells were treated with 4 mM 2DG and/or 53.8 μM FA for 24 h and then exposed to 2 Gy irradiation. Compared to cells that were 2 Gy-irradiated alone (50%), FA and 2DG with radiation (FA+2DG+IR) showed additional decrease in cell viability (15%). This has been further validated by decreased (86%) colony formation in 2DG+FA+IR group compared to 2DG (29%), FA (24%) and IR (37%) group alone. Increased apoptotic cells (84%) in 2DG+FA+IR group further confirm the radiosensitizing property of 2DG or FA. In NCI-H460 cells 2DG decreased NADPH levels (10%) and FA increased ROS levels leading to enhanced oxidative damage in the 2DG+FA+IR group. This was reflected as altered mitochondrial membrane potential, increased lipid peroxidative markers (TBARS), DNA damage and decreased intracellular glutathione (GSH) levels in combined treatment groups when compared to radiation or 2DG or FA treatment alone. The present study suggests that FA and 2DG act by increasing oxidative damage in NCI-H460 cells.

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Enhancement of radiation-induced oxidative stress and cytotoxicity in tumor cells by ellagic acid

Cited by 123 publications

References 43 publications

Modulatory Role of Selenium Nanoparticles and Grape Seed Extract Mixture on Oxidative Stress Biomarkers in Diabetic Irradiated Rats.

Modulatory Role of Selenium Nanoparticles and Grape Seed Extract Mixture on Oxidative Stress Biomarkers in Diabetic Irradiated Rats.

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

2-deoxy-D-glucose combined with ferulic acid enhances radiation response in non-small cell lung carcinoma cells

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