Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

Abstract

The present study was designed to investigate the oxidative stress, which is due to the effect of low doses of gamma irradiation. Animals were divided into 6 groups, where the first group kept as control group, while 2 nd , 3 rd , 4 th , 5 th and 6 th groups were exposed to gamma ray at 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy once weekly for one month respectively. Rats were subjected to gamma radiation and nitric oxides (NO), superoxide dismutase (SOD), malondialdehyde, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and white blood cells (WBCs) were measured. SOD recorded highly significant decrease with percent change -22.7%, -25.01%, -55.01% -55.4% and -56.38% respectively in groups which were exposed to 1.5, 2, 2.5, 3 and 3.5 Gy. MDA serum level have a significant increase in groups which were exposed to successive doses of 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 25.7% , 41.8% 59.1% 65.6% 75.8% consequently as compared to control group. Nitric oxide serum level is markedly showed highly significant increase in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 59.69%, 74.17%, 87.1%, 120.1% and 130.6% consequently as compared to control group. ALT and AST recorded highly significant increase in rats exposure to successive doses of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. On the other hand WBCs recorded highly significant decreased in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. WBCs recorded percent change -26.11%, -32.3%, -47.8%, -50.1% and -53.6% respectively as compared to control group.

Perturbed transcriptional profiles after chronic low dose rate radiation in mice

“…Higher numbers of specific oxidative stress-related DEGs were expressed in the HDR group than in the MDR and the LDR groups (Fig 7). In agreement with our HDR results, rats exposed to acute high dose rates of gamma radiation (1.5-3.5 Gy) once a week for one month expressed significantly changed antioxidant enzyme activities in liver and skeletal muscle tissues one month after radiation, which is indicative of oxidative stress [79]. Whether exposure to chronic low dose rate ionizing radiation in our study has led to an imbalance between the ROS generation and the antioxidant capacity, reaching a state of liver oxidative stress, is unclear.…”

Fucoxanthin alters the apelin-13/APJ pathway in certain organs of γ-irradiated mice

“…for 3 days before γ-ray exposure to stimulate and impose a pre-conditioning status in normal cells to overcome and sustain the subsequent detrimental effects induced by irradiation in order to achieve radioprotection and adaptive responses of the tissues exposed. The γ-radiation dose was chosen according to the study of Zakaria [ 18 ] that aimed to determine the hazardous effects of low successive doses during exposure to γ-irradiation of many workers in the medical, industrial and petroleum fields who may be exposed during a small radiation accident to low or moderate γ-radiation doses (1.5, 2, 2.5, 3 and 3.5 Gy). These doses lead to acute effects on health efficiency and performance of organisms.…”