Enhancement of protease production by Pseudomonas aeruginosa isolated from dairy effluent sludge and determination of its fibrinolytic potential

Raj, A. B. J.; Khess, Nancy; Pujari, Namrata; Bhattacharya, Sourav; Das, Arijit; Rajan, S.

doi:10.1016/s2221-1691(12)60506-1

Cited by 31 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the industrial enzymes, proteases are one of the most important groups contributing to the largest sales after carbohydrases and they account for a quarter of the total global enzyme production (Kalaiarasi and Sunitha, 2009). They are ubiquitous and widely distributed in plants, animals and microbes with microbes as major sources among all (Amrita Raj et al, 2012). Fungi harbor a variety of proteolytic enzymes and are important sources compared to bacteria because of their ability to grow at extremes of temperature, pH and as well use a wide variety of substrates as nutrients.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability

Lanka¹,

Anjali²,

Pydipalli³

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Proteases constitute most important enzymes owing to their wide variety of functions and have immense applications in various industries viz., medical, pharmaceutical, biotechnology, leather, detergent, and food industries. Despite of their wide spread occurrence in various sources, microorganisms present remarkable potential for proteolytic enzymes production due to their extensive biochemical diversity and susceptibility to genetic manipulation. The present study was aimed at isolating alkaline protease producing fungal members from dairy form effluents, designing the process variables for maximizing the protease production and determining the fibrinolytic potential of the partially purified alkaline protease. To achieve the specified objectives, the dairy form effluent was processed for the isolation of proteolytic fungi using suitable microbiological medium. All the fungal isolates were screened for their protease producing ability and the isolate showing highest alkaline protease production was selected for further studies. Optimization of different fermentative variables like carbon, nitrogen sources, pH, temperature and incubation period were carried out to enhance enzyme production. Ammonium sulphate fractionation was employed to partially purify the enzyme following which its fibrinolytic potential was determined. Based on morphological and microscopic studies, the selected fungal isolate was identified as Aspergillus niger. Optimization studies using OVAT (one variable at a time) method revealed an enhanced protease production in the presence of fructose as additional carbon source and ammonium sulphate as nitrogen source. The optimum incubation period, temperature and pH for enzyme production by the selected fungal isolate was found to be 92 h, 50°C and 10, respectively. The partially purified alkaline protease was efficient in the removal of blood stains emphasizing its fibrinolytic ability. An alkaline protease producing Fungal sp. was screened and isolated from dairy form effluent and it was found to be efficient in the removal of blood stains proving its fibrinolytic potential. Enzymes produced from microorganisms that can survive under extremes of pH could be particularly useful for commercial applications under high alkaline conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability

Lanka¹,

Anjali²,

Pydipalli³

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…With increase in inoculum concentration to 15% and temperature to 45°C, protease activity decreased. This could be due to the fact that the optimum temperature for the growth of H. fuscoatra MTCC 1409 is 40±2°C and increase in inoculum concentration will lead to decrease in nutrients for growth of fungi (Raj et al, 2012).…”

Section: Temperature Verses Inoculum Concentrationmentioning

confidence: 99%

POULTRY DROPPING BASED BIODIGESTED SLURRY FOR PROTEASE PRODUCTION BY Humicola fuscoatra MTCC 1409: OPTIMIZATION AND KINETIC STUDY

Goyal¹,

Dar²,

Phutela³

2018

JEBAS

View full text Add to dashboard Cite

Protease is one of the most important groups of commercially produced enzymes. This study was aimed at the optimization and kinetics of protease production from poultry dropping based biodigested slurry by Humicola fuscoatra MTCC 1409. Four significant variables (pH, temperature, slurry concentration and inoculum concentration) were considered for optimization both by one variable at a time approach and response surface methodology. The maximum protease production in the poultry dropping based biodigested slurry was (531±1.37 U g -1 ) under the optimum conditions of pH (5), temperature (40°C), slurry concentration (25%) and inoculum concentration (10%). The protease production was found to be 3.38 fold higher under optimized conditions as compared to the non-optimized ones. The thermal inactivation of protease produced from biodigested slurry was investigated kinetically within temperature range of 30-70°C. The irreversible inactivation was well described by first order kinetics with k values increasing between 0.0028 to 0.0071 min -1 and t1/2 decreasing from247.70 to 98.10 mins.At higher temperature, there was significant decrease in residual activity. The activation energy, enthalpy, Gibbs free energy and entropy range calculated on the basis of residual activity experiments conducted at temperature range of 30-70°C was found to be 21.29, 18.44 to 18.78 kJ mol -1

show abstract

“…Proteases are produced by a wide range of sources (Sharma et al, 2017). The majority of commercially available enzymes are obtained from microbial origin (Raj et al, 2012). Microbial proteases play a vital role in biotechnological processes and constitute one of the most important groups of industrial enzymes, selling product segment in the global market accounting for 60% market share (Kumar et al, 2014).Bacillus genus is one of the most important producers of extracellular proteases and industrial sectors very often use Bacillus species for the production of proteases (Contesini et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of alkaline protease from novel bacterium Bacillus cereus GVK21 under submerged fermentation

Keshavamurthy¹,

Vishwanatha²,

M³

et al. 2018

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

Alkaline proteases are an important class of enzymes with potential industrial and commercial applications. In the present study, 52 bacterial isolates from various soil samples have been evaluated for the production of extracellular protease and selected one potent isolate based on maximum casein hydrolysis. Further, it is identifi ed as Bacillus cereus GVK21 based on biochemical characteristics and 16S rRNA gene sequence analysis (KY659318). This organism produced 136 U/mL of protease within 48 hrs of incubation. Maximum production of protease was recorded at pH 9 and a temperature of 40°C. The present study is attempted to exploit new economical media, based on agro wastes recipes for the increased production of alkaline proteases. B.cereus GVK21 produced high levels of protease (1762 U/ ml) on groundnut oil cake (1.5 %) as a substrate.The results of the study show that this isolate can be further exploited for commercial production of protease.

show abstract

Enhancement of protease production by Pseudomonas aeruginosa isolated from dairy effluent sludge and determination of its fibrinolytic potential

Cited by 31 publications

References 15 publications

Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability

Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability

POULTRY DROPPING BASED BIODIGESTED SLURRY FOR PROTEASE PRODUCTION BY Humicola fuscoatra MTCC 1409: OPTIMIZATION AND KINETIC STUDY

Enhanced production of alkaline protease from novel bacterium Bacillus cereus GVK21 under submerged fermentation

Contact Info

Product

Resources

About