Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability

Abstract

Proteases constitute most important enzymes owing to their wide variety of functions and have immense applications in various industries viz., medical, pharmaceutical, biotechnology, leather, detergent, and food industries. Despite of their wide spread occurrence in various sources, microorganisms present remarkable potential for proteolytic enzymes production due to their extensive biochemical diversity and susceptibility to genetic manipulation. The present study was aimed at isolating alkaline protease producing fungal members from dairy form effluents, designing the process variables for maximizing the protease production and determining the fibrinolytic potential of the partially purified alkaline protease. To achieve the specified objectives, the dairy form effluent was processed for the isolation of proteolytic fungi using suitable microbiological medium. All the fungal isolates were screened for their protease producing ability and the isolate showing highest alkaline protease production was selected for further studies. Optimization of different fermentative variables like carbon, nitrogen sources, pH, temperature and incubation period were carried out to enhance enzyme production. Ammonium sulphate fractionation was employed to partially purify the enzyme following which its fibrinolytic potential was determined. Based on morphological and microscopic studies, the selected fungal isolate was identified as Aspergillus niger. Optimization studies using OVAT (one variable at a time) method revealed an enhanced protease production in the presence of fructose as additional carbon source and ammonium sulphate as nitrogen source. The optimum incubation period, temperature and pH for enzyme production by the selected fungal isolate was found to be 92 h, 50°C and 10, respectively. The partially purified alkaline protease was efficient in the removal of blood stains emphasizing its fibrinolytic ability. An alkaline protease producing Fungal sp. was screened and isolated from dairy form effluent and it was found to be efficient in the removal of blood stains proving its fibrinolytic potential. Enzymes produced from microorganisms that can survive under extremes of pH could be particularly useful for commercial applications under high alkaline conditions.

Metabolomic profiling and its association with the bio-efficacy of Aspergillus niger strain against Fusarium wilt of guava

“…In our study, 11 strains used as antagonists were confirmed as A. niger based on morphological and molecular characteristics. The studied morphological characteristics, such as colony morphology, conidia, vesicle, and conidiophore nature, confirmed the previous reports (McClenny, 2005 ; Diba et al, 2007 ; Varga et al, 2011 ; Lanka et al, 2017 ; Atallah et al, 2022 ). The conventional morphological approaches may not perform well for the accurate identification of the genus Aspergillus up to the species level.…”

Characterization and immobilization of protease secreted by the fungus Moorella speciosa

“…However, several studies with ascomycete fungi have been carried out to produce different enzymes, with emphasis on genera Aspergillus and Penicillium (Zhu et al 2009;Morya and Yadav 2010;Yadav et al 2011;Lanka et al 2017). The proteolytic enzyme produced by these fungi has the ability to degrade proteolytic materials; thus, it is applicable to many fields (Chandrasekaran et al 2015).…”

Lentinus Villosus Klotzsch (1833) Am 169: A Natural and Renewable Source of Alkaline Protease / Lentinus Villosus Klotzsch (1833) Am 169: Uma Fonte Natural E Renovável De Protease Alcalina

“…Due to broad biotechnological applications, proteases correspond to 65% of the enzyme market (Kummari & Prasad, 2015;Lanka et al, 2017). In the industrial sector, the peptidases are used in the manufacture of cheeses, beverages, softening of meats and bakery products.…”