Enhancement of Production of d-Glucosamine in Escherichia coli by Blocking Three Pathways Involved in the Consumption of GlcN and GlcNAc

Li, Piwu; Li, Xu; Zhao, Wei; Dong, Ruizhen; Li, Kang; Fan, Han; Wang, Ruiming; Wang, Junqing; Lv, Maocui

doi:10.1007/s12033-020-00257-9

Cited by 3 publications

(1 citation statement)

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“…[ 30 ] Three strategies have been applied to regulate flux direction, such as decoupling cell growth and product synthesis, inhibiting by‐product synthesis, and cutting off the competitive branching pathways. [ 22 , 31 ] These strategies have achieved to shift flux direction from the competitive pathways to product synthesis pathways, but they cannot reasonably redistribute the metabolic flux derived from the competitive pathways for product synthesis, due to the fact that long‐chain molecules usually consist of multiple precursor pools. For example, hyaluronic acid contains two precursor pools.…”

Section: Discussionmentioning

confidence: 99%

Reprogramming Metabolic Flux in Escherichia Coli to Enhance Chondroitin Production

Zhao,

Li,

Guo

et al. 2023

Advanced Science

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Reprogramming metabolic flux is a promising approach for constructing efficient microbial cell factories (MCFs) to produce chemicals. However, how to boost the transmission efficiency of metabolic flux is still challenging in complex metabolic pathways. In this study, metabolic flux is systematically reprogrammed by regulating flux size, flux direction, and flux rate to build an efficient MCF for chondroitin production. The ammoniation pool for UDP‐GalNAc synthesis and the carbonization pool for UDP‐GlcA synthesis are first enlarged to increase flux size for providing enough precursors for chondroitin biosynthesis. Then, the ammoniation pool and the carbonization pool are rematched using molecular valves to shift flux direction from cell growth to chondroitin biosynthesis. Next, the adaptability of polymerization pool with the ammoniation and carbonization pools is fine‐tuned by dynamic and static valve‐based adapters to accelerate flux rate for polymerizing UDP‐GalNAc and UDP‐GlcA to produce chondroitin. Finally, the engineered strain E. coli F51 is able to produce 9.2 g L−1 chondroitin in a 5‐L bioreactor. This strategy shown here provides a systematical approach for regulating metabolic flux in complex metabolic pathways for efficient biosynthesis of chemicals.

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Section: Discussionmentioning

confidence: 99%