Enhancement of piscine orthoreovirus-2 DNA vaccine potency by linkage of antigen gene to a trigger factor gene or signal peptide genes

Matsuyama, Tomomasa; Takano, Tomokazu; Honjo, Miho; Kikuta, Takumi; Nawata, Akatsuki; Kumagai, Akira; Honda, Ryo; Sakai, Takamitsu; Kurita, Jun; Terashima, Sachiko; Yamazaki, Masatoshi; Matsuura, Yuta; Nishizawa, Yuko; Nakayasu, Chihaya

doi:10.1016/j.aquaculture.2020.736163

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…One BALB/c mouse (Nippon Crea) was immunized with this recombinant protein according to the standard method to obtain an antiserum against the protein (anti‐σ1 mouse polyclonal antibody, pAb PRV2‐σ1). In addition, σ1 protein was fused to glutathione S‐transferase (GST) in a wheat cell‐free system (Matsuyama et al., 2021) and used for western blot analysis. The reaction solution of the protein conjugate was directly loaded onto the SDS‐PAGE gels for Western blotting without purification.…”

Section: Methodsmentioning

confidence: 99%

Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2)

Takano,

Miwa,

Matsuyama

et al. 2024

Journal of Fish Diseases

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The relationship of histopathological changes and the infection of Piscine orthoreovirus 2 (PRV‐2) was investigated in coho salmon that were suffering from the erythrocytic inclusion body syndrome (EIBS). Immunohistochemical observations revealed abundant σ1 protein of PRV‐2 in the spongy layer of the ventricle of the heart, where severe myocarditis was observed. In the spleen, the virus protein was detected in many erythrocytes, some of which were spherical‐shaped and apparently dead. The number of erythrocytes was decreased in the spleen compared to the apparently healthy fish. The virus protein was also detected in some erythrocytes in blood vessels. The viral protein was often detected in many macrophages ingesting erythrocytes or dead cell debris in the spleen or in the kidney sinusoids. Large amounts of the viral genomic segment L2 were also detected in these organs by RT‐qPCR. Many necrotic foci were found in the liver, although the virus protein was not detected in the hepatocytes. These results suggest that the primary targets of PRV‐2 are myocardial cells and erythrocytes and that clinical symptoms such as anaemia or jaundice and histopathological changes such as myocarditis in EIBS‐affected coho salmon are caused by PRV‐2 infection.

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Section: Methodsmentioning

confidence: 99%

Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2)

Takano,

Miwa,

Matsuyama

et al. 2024

Journal of Fish Diseases

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“…At present, there are no commercially available vaccines against HSMI in the market, and research and evaluation of PRV vaccines are still incipient. Only four studies performed on Atlantic salmon and Coho salmon have been reported ( 76 – 79 ). Wessel et al.…”

Section: Prv Vaccinesmentioning

confidence: 99%

Sleeping With the Enemy? The Current Knowledge of Piscine Orthoreovirus (PRV) Immune Response Elicited to Counteract Infection

et al. 2022

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Piscine orthoreovirus (PRV) is a virus in the genus Orthoreovirus of the Reoviridae family, first described in 2010 associated with Heart and Skeletal Muscle Inflammation (HSMI) in Atlantic salmon (Salmo salar). Three phases of PRV infection have been described, the early entry and dissemination, the acute dissemination phase, and the persistence phase. Depending on the PRV genotype and the host, infection can last for life. Mechanisms of immune response to PRV infection have been just beginning to be studied and the knowledge in this matter is here revised. PRV induces a classical antiviral immune response in experimental infection of salmonid erythrocytes, including transcriptional upregulation of ifn-α, rig-i, mx, and pkr. In addition, transcript upregulation of tcra, tcrb, cd2, il-2, cd4-1, ifn-γ, il-12, and il-18 has been observed in Atlantic salmon infected with PRV, indicating that PRV elicited a Th1 type response probably as a host defense strategy. The high expression levels of cd8a, cd8b, and granzyme-A in PRV-infected fish suggest a positive modulatory effect on the CTL-mediated immune response. This is consistent with PRV-dependent upregulation of the genes involved in antigen presentation, including MHC class I, transporters, and proteasome components. We also review the potential immune mechanisms associated with the persistence phenotype of PRV-infected fish and its consequence for the development of a secondary infection. In this scenario, the application of a vaccination strategy is an urgent and challenging task due to the emergence of this viral infection that threatens salmon farming.

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Programmable Oligonucleotide-Peptide Complexes: Synthesis and Applications

Zhou

Ding

et al. 2021

Chem. Res. Chin. Univ.

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Enhancement of piscine orthoreovirus-2 DNA vaccine potency by linkage of antigen gene to a trigger factor gene or signal peptide genes

Cited by 4 publications

References 58 publications

Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2)

Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2)

Sleeping With the Enemy? The Current Knowledge of Piscine Orthoreovirus (PRV) Immune Response Elicited to Counteract Infection

Programmable Oligonucleotide-Peptide Complexes: Synthesis and Applications

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