Enhancement of neurite outgrowth in PC12 cells by iron oxide nanoparticles

Kim, Jeong Ah; Lee, Jun Young; Kim, Byung Hyo; Rhee, Won Jong; Yoon, Sungjun; Hyeon, Taeghwan; Park, Tai Hyun

doi:10.1016/j.biomaterials.2011.01.019

Cited by 113 publications

(93 citation statements)

References 25 publications

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“…Ciofani et al showed a ~40% increase in neuronal cell outgrowth using piezoelectric boron nitride nanotubes excited by ultrasound 7 . Similarly, endocytosed iron oxide nanoparticles in PC12 cells have been reported to enhance neurite differentiation in a dose-dependent manner, due to the activation of cell adhesion molecules with the iron oxide 8 .…”

Section: Introductionmentioning

confidence: 94%

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Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Paviolo

McArthur

Stoddart

2015

JoVE

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Recent studies have demonstrated that nerves can be stimulated in a variety of ways by the transient heating associated with the absorption of infrared light by water in neuronal tissue. This technique holds great potential for replacing or complementing standard stimulation techniques, due to the potential for increased localization of the stimulus and minimization of mechanical contact with the tissue. However, optical approaches are limited by the inability of visible light to penetrate deep into tissues. Moreover, thermal modelling suggests that cumulative heating effects might be potentially hazardous when multiple stimulus sites or high laser repetition rates are used. The protocol outlined below describes an enhanced approach to the infrared stimulation of neuronal cells. The underlying mechanism is based on the transient heating associated with the optical absorption of gold nanorods, which can cause triggering of neuronal cell differentiation and increased levels of intracellular calcium activity. These results demonstrate that nanoparticle absorbers can enhance and/or replace the process of infrared neural stimulation based on water absorption, with potential for future applications in neural prostheses and cell therapies. Video LinkThe video component of this article can be found at

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Section: Introductionmentioning

confidence: 94%

“…live/dead, MTS, MTT, etc.) while histological analysis is often performed during tissue experiments 7,8,12,15 .…”

Section: Discussionmentioning

confidence: 99%

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Paviolo

McArthur

Stoddart

2015

JoVE

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“…The release of free iron ions affects distinct subcellular processes -eg, it can enforce mitochondrial dysfunction through the production of reactive oxygen species -but can also promote neurite outgrowth under stimulus conditions. 32,33 Iron deposits in activated microglia have also been found to be a notable feature in neurodegenerative diseases, including multiple sclerosis and Alzheimer's and Parkinson's disease, leading to homeostatic imbalance and even cell death. 32,34,35 The use of SPIOs as contrast agents in MRI is especially critical, because whether or not particles interact with brain …”

Section: Iron Oxide Nanoparticlesmentioning

confidence: 99%

“…32,35 On the other hand, it has been shown that iron ions from certain nanoparticles enhance neuronal differentiation and survival of immortal PC12 neurons, thereby promoting neurite outgrowth in a dose-dependent manner. 33 In our study, we quantified total numbers of neurites of primary neurons, and did not differentiate between dendrites or axons. It would be of certain relevance in terms of therapeutic interventions using specific SPIOs and concentrations if these particles induce dendritic or axonal outgrowth, eg, in the treatment of neurodegenerative diseases.…”

Section: Neuronal Vitality Is Modulated By Spiosmentioning

confidence: 99%

New findings about iron oxide nanoparticles and their different effects on murine primary brain cells

Neubert¹,

Wagner²,

Kiwit³

et al. 2015

IJN

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The physicochemical properties of superparamagnetic iron oxide nanoparticles (SPIOs) enable their application in the diagnostics and therapy of central nervous system diseases. However, since crucial information regarding side effects of particle–cell interactions within the central nervous system is still lacking, we investigated the influence of novel very small iron oxide particles or the clinically approved ferucarbotran or ferumoxytol on the vitality and morphology of brain cells. We exposed primary cell cultures of microglia and hippocampal neurons, as well as neuron–glia cocultures to varying concentrations of SPIOs for 6 and/or 24 hours, respectively. Here, we show that SPIO accumulation by microglia and subsequent morphological alterations strongly depend on the respective nanoparticle type. Microglial viability was severely compromised by high SPIO concentrations, except in the case of ferumoxytol. While ferumoxytol did not cause immediate microglial death, it induced severe morphological alterations and increased degeneration of primary neurons. Additionally, primary neurons clearly degenerated after very small iron oxide particle and ferucarbotran exposure. In neuron–glia cocultures, SPIOs rather stimulated the outgrowth of neuronal processes in a concentration- and particle-dependent manner. We conclude that the influence of SPIOs on brain cells not only depends on the particle type but also on the physiological system they are applied to.

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“…In particular, multifunctional nanoparticle platforms have gained considerable interest for their use in targeted drug delivery systems [1].…”

Section: I-introductionmentioning

confidence: 99%

Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5- Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line

Hajikarimi

Khoei

Khoee³

et al. 2014

IEEE Trans.on Nanobioscience

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The purpose of this study was to investigate the uptake and cytotoxic effects of magnetic poly lactic-co-glycolic acid (PLGA)-coated iron oxide nanoparticles as a carrier of 5-fluorouracil (5-FU) and X-ray on the level of proliferation capacity of DU145 prostate carcinoma cell line in monolayer culture. Following monolayer culture, DU 145 cells were treated with different concentrations of 5-FU or 5-FU loaded nanoparticles for 24 h and 2Gy X-ray (6 Mega-voltage (MV)). The rate of nanoparticles penetration was then measured using atomic adsorption spectroscopy (AAS). The cytotoxicity effect of these nanoparticles with/ without X-ray radiation was evaluated using colony formation assay. Spectroscopy results showed that iron content and therefore the cellular uptake of 5-FU loaded nanoparticles increased with increasing nanoparticle concentrations. Further, the proliferation capacity of the cells decreased with the increase of 5-FU and 5- FU loaded nanoparticle concentrations in combination with X-ray radiation. However the extent of reduction in colony number following treatment with 5-FU-loaded nanoparticles in combination with 2Gy of megavoltage X-ray radiation was significantly more than for free 5-FU. Thus, drug-loaded nanoparticles could deliver 5-FU more efficiently into the cells. PLGA coated iron oxide nanoparticles are therefore effective drug delivery vehicles for 5-FU. PLGA coated iron oxide nanoparticles are biocompatible and this coating is an appropriate surface that can penetrate into the cells.

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Enhancement of neurite outgrowth in PC12 cells by iron oxide nanoparticles

Cited by 113 publications

References 25 publications

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

New findings about iron oxide nanoparticles and their different effects on murine primary brain cells

Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5- Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line

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Enhancement of neurite outgrowth in PC12 cells by iron oxide nanoparticles

Cited by 113 publications

References 25 publications

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

New findings about iron oxide nanoparticles and&nbsp;their different effects on murine primary brain cells

Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5- Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line

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New findings about iron oxide nanoparticles and their different effects on murine primary brain cells