Enhancement of mechanical strength of TCP-alginate based bioprinted constructs

Song, Jie-Liang; Fu, Xiaoxiao; Raza, Ali; Shen, Naian; Xue, Yi; Wang, Huajie; Wang, Jin‐Ye

doi:10.1016/j.jmbbm.2019.103533

Cited by 16 publications

(6 citation statements)

References 47 publications

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“…This is because the presence of hard β-TCP particles might enhance the shear stress of the bioink. 43 In addition, during the cell encapsulation and printing process, the nano β-TCP particles might cause physical damage to the cells. The cell proliferation assay exhibited good cell metabolic activity in the four groups.…”

Section: Discussionmentioning

confidence: 99%

“…In the cell biological assay, the Live/Dead assay showed the cell viability of 0T and 1T was higher than that of 3T and 5T on day 1. This is because the presence of hard β‐TCP particles might enhance the shear stress of the bioink 43 . In addition, during the cell encapsulation and printing process, the nano β‐TCP particles might cause physical damage to the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, controversy persists regarding the optimal concentration of β‐TCP for osteogenic potential in extrusion‐based bioprinting. Song et al 43 observed that when using a composite bioink of sodium alginate and β‐TCP for bioprinting, setting the sodium alginate concentration at 2% (w/v) and increasing the β‐TCP ratio (from β‐TCP: alginate = 0:4 to β‐TCP: alginate = 8:4) resulted in the decrease in cell viability within the printed constructs. However, another study indicated that a collagen‐β‐TCP composite bioink with β‐TCP concentrations as high as 20% maintained over 90% cell viability post‐printing and exhibited significant expression of osteogenic genes 44 .…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Zhang,

Suttapreyasri,

Leethanakul

et al. 2024

J Biomedical Materials Res

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Bone tissue is a highly vascularized tissue. When constructing tissue‐engineered bone models, both the osteogenic and angiogenic capabilities of the construct should be carefully considered. However, fabricating a vascularized tissue‐engineered bone to promote vascular formation and bone generation, while simultaneously establishing nutrition channels to facilitate nutrient exchange within the constructs, remains a significant challenge. Triaxial bioprinting, which not only allows the independent encapsulation of different cell types while simultaneously forming nutrient channels, could potentially emerge as a strategy for fabricating vascularized tissue‐engineered bone. Moreover, bioinks should also be applied in combination to promote both osteogenesis and angiogenesis. In this study, employing triaxial bioprinting, we used a blend bioink of gelatin methacryloyl (GelMA), sodium alginate (Alg), and different concentrations of nano beta‐tricalcium phosphate (nano β‐TCP) encapsulated MC3T3‐E1 preosteoblasts as the outer layer, a mixed bioink of GelMA and Alg loaded with human umbilical vein endothelial cells (HUVEC) as the middle layer, and gelatin as a sacrificial material to form nutrient channels in the inner layer to fabricate vascularized bone constructs simulating the microenvironment for bone and vascular tissues. The results showed that the addition of nano β‐TCP could adjust the mechanical, swelling, and degradation properties of the constructs. Biological assessments revealed the cell viability of constructs containing different concentrations of nano β‐TCP was higher than 90% on day 7, The cell‐laden constructs containing 3% (w/v) nano β‐TCP exhibited better osteogenic (higher Alkaline phosphatase activity and larger Osteocalcin positive area) and angiogenic (the gradual increased CD31 positive area) potential. Therefore, using triaxial bioprinting technology and employing GelMA, Alg, and nano β‐TCP as bioink components could fabricate vascularized bone tissue constructs, offering a novel strategy for vascularized bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Zhang,

Suttapreyasri,

Leethanakul

et al. 2024

J Biomedical Materials Res

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“…Moreover, some side effects, including allergies, were reported in the case of an insufficient amount of moisture in the wound bed [192]. The mechanical strength of alginate can also be insufficient for the formulation of materials other than flat dressings, as more complex constructs and scaffolds can collapse [193,194]. Finally, the sterilization of alginate-based dressings can be also challenging, as literature reports indicate loss of mechanical properties and decreased swelling ability upon steam sterilization [195], impaired swelling and chemical degradation upon irradiation [196,197], and changes in mechanical characteristics as a result of supercritical carbon dioxide sterilization [198].…”

Section: Discussionmentioning

confidence: 99%

Alginate-Based Materials Loaded with Nanoparticles in Wound Healing

et al. 2023

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Alginate is a naturally derived polysaccharide widely applied in drug delivery, as well as regenerative medicine, tissue engineering and wound care. Due to its excellent biocompatibility, low toxicity, and the ability to absorb a high amount of exudate, it is widely used in modern wound dressings. Numerous studies indicate that alginate applied in wound care can be enhanced with the incorporation of nanoparticles, revealing additional properties beneficial in the healing process. Among the most extensively explored materials, composite dressings with alginate loaded with antimicrobial inorganic nanoparticles can be mentioned. However, other types of nanoparticles with antibiotics, growth factors, and other active ingredients are also investigated. This review article focuses on the most recent findings regarding novel alginate-based materials loaded with nanoparticles and their applicability as wound dressings, with special attention paid to the materials of potential use in the treatment of chronic wounds.

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“…Besides natural polymers, synthetic polymers are also widely used in biofabrication, including polyethylene glycol (PEG) [ [36] , [37] , [38] , [39] ], polycaprolactone (PCL) [ [40] , [41] , [42] ], polyvinylpyrrolidone (PVP) [ [43] , [44] , [45] ], poly(l-lactic) acid (PLA) [ [46] , [47] , [48] ] and poly(lactic-co-glycolic) acid (PLGA) [ 49 ]. They can be tuned to comply with tissue-specific degradation and mechanical property requirement of the target tissues and organs.…”

Section: Chemically-defined Ecm and Synthetic Ecm Analoguesmentioning

confidence: 99%

Biofabrication methods for reconstructing extracellular matrix mimetics

Aazmi,

Zhang,

Mazzaglia

et al. 2024

Bioactive Materials

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Enhancement of mechanical strength of TCP-alginate based bioprinted constructs

Cited by 16 publications

References 47 publications

Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Alginate-Based Materials Loaded with Nanoparticles in Wound Healing

Biofabrication methods for reconstructing extracellular matrix mimetics

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