Biofabrication methods for reconstructing extracellular matrix mimetics

Aazmi, Abdellah; Zhang, Duo; Mazzaglia, Corrado; Yu, Mengfei; Wang, Zhen; Yang, Huayong; Huang, Yan Yan Shery; Ma, Liang

doi:10.1016/j.bioactmat.2023.08.018

Cited by 8 publications

(4 citation statements)

References 348 publications

(441 reference statements)

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“…GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM), because of the presence of cell-adhesion and matrix metalloproteinase (MMP) responsive peptide motifs. With the excellent biological properties and tunable physical characteristics, GelMA hydrogels have a wide range of biomedical applications, such as 3D bioprinting [ 60 , 62 ], tissue engineering [ 61 , 63 ], drug delivery [ 64 ], and biomedical microrobot [ 57 ] et al Additionally, lots of efforts have been devoted to combining GelMA-based hydrogels and functional nanomaterials, which aims to endow GelMA with enhanced physical-chemical and biological properties [ 63 ].…”

Section: Methodsmentioning

confidence: 99%

Oral administration microrobots for drug delivery

Ren,

Hu,

Qin

et al. 2024

Bioactive Materials

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Section: Methodsmentioning

confidence: 99%

Oral administration microrobots for drug delivery

Ren,

Hu,

Qin

et al. 2024

Bioactive Materials

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“…3D bioprinting, arising from additive manufacturing, involves the automated and precise deposition of cells, biomaterials, and bioactive molecules, collectively forming a “bioink.” It addresses the limitations of conventional 2D platforms by enabling the creation of scaffolds with tailored structural and biochemical properties ( Jain et al, 2022 ; Lam et al, 2023 ; Gold et al, 2019 ; Matai et al, 2020 ; Ahadian and Khademhosseini, 201 8; Agarwal et al, 2020 ; Noroozi et al, 2023 ; Aazmi et al, 2024 ). This capability is pivotal for generating in vitro models that accurately mimic the intricate features of native tissues, facilitating regenerative medical endeavors ( Nam et al, 2015 ; Langhans, 2018 ; Bédard et al, 2020 ; Hwang et al, 2021 ).…”

Section: Elrs For 3d In Vitro Models In Regenerati...mentioning

confidence: 99%

Contribution of the ELRs to the development of advanced in vitro models

Puertas-Bartolomé,

Venegas-Bustos,

Acosta

et al. 2024

Front. Bioeng. Biotechnol.

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Developing in vitro models that accurately mimic the microenvironment of biological structures or processes holds substantial promise for gaining insights into specific biological functions. In the field of tissue engineering and regenerative medicine, in vitro models able to capture the precise structural, topographical, and functional complexity of living tissues, prove to be valuable tools for comprehending disease mechanisms, assessing drug responses, and serving as alternatives or complements to animal testing. The choice of the right biomaterial and fabrication technique for the development of these in vitro models plays an important role in their functionality. In this sense, elastin-like recombinamers (ELRs) have emerged as an important tool for the fabrication of in vitro models overcoming the challenges encountered in natural and synthetic materials due to their intrinsic properties, such as phase transition behavior, tunable biological properties, viscoelasticity, and easy processability. In this review article, we will delve into the use of ELRs for molecular models of intrinsically disordered proteins (IDPs), as well as for the development of in vitro 3D models for regenerative medicine. The easy processability of the ELRs and their rational design has allowed their use for the development of spheroids and organoids, or bioinks for 3D bioprinting. Thus, incorporating ELRs into the toolkit of biomaterials used for the fabrication of in vitro models, represents a transformative step forward in improving the accuracy, efficiency, and functionality of these models, and opening up a wide range of possibilities in combination with advanced biofabrication techniques that remains to be explored.

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“…Electrospun scaffolds can be tailored to match the mechanical properties of specific tissues by selecting appropriate polymer compositions, adjusting fiber diameter and density, or incorporating reinforcing materials like nanoparticles or nanofibers. By incorporating these biological mimetics into tissue engineering electrospun scaffolds, researchers can create novel tissue engineering scaffolds [54] .…”

Section: Biological Approach For Mimetic Of Cartilage Tissue Engineer...mentioning

confidence: 99%

Modification of Biodegradable Polymer Nanofibers for Cartilage Tissue Engineering Applications: A Review

Shanmugam

2024

BAS

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Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physiochemical factors to improve or replace biological functions at the injured site. The designing of a biomaterial that can mimic the three-dimensional tissues in vivo is still challenging. Biodegradable polymers are used for the development of tissue engineering constructs in the form of sponges, films, and macroporous scaffolds, which do not influence cell fate processes such as cell differentiation, migration, and proliferation. Biodegradable polymer nanofibers fabricated by electrospinning have gathered great attention in tissue engineering applications. The electrospun materials have a nanofibrous morphology that is closest to the natural extracellular matrix (ECM). The electrospun material is composed of three-dimensional networks of nanosized fibrous materials that mimic an extracellular matrix such as collagen, elastin, and keratin. These polymers fabricated in the form of fibers in nanosize cause a more favorable microenvironment for cells. We prepared the PLGA/PPG (polylactic-co-glycolic acid/polypropylene glycol) nanofibers by electrospinning technique. PLGA (85:15)/PLGA (75:25) nanofibers are hydrophobic, which can be minimized by the addition of PPG to give better hydrophilicity for cell adhesion for tissue engineering constructs. The morphology of the electrospun fibers of the composite of PLGA and PPG was observed using scanning electron microscopy (SEM). The results proved that the small amount of polypropylene glycol polymer to the polylactic-co-glycolic acid (PLGA 85:15) drastically improves the hydrophilicity of the electrospun nanofibers. The addition of a small amount of hydrophilic polymer to the biodegradable hydrophobic polymer increases the hydrophilic property and can be used for nanofiber-based tissue engineering constructs. In addition, the biomimetic approach for tissue engineering scaffolds for cartilage repair has been discussed.

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Biofabrication methods for reconstructing extracellular matrix mimetics

Cited by 8 publications

References 348 publications

Oral administration microrobots for drug delivery

Oral administration microrobots for drug delivery

Contribution of the ELRs to the development of advanced in vitro models

Modification of Biodegradable Polymer Nanofibers for Cartilage Tissue Engineering Applications: A Review

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