Enhancement of lipid accumulation and docosahexaenoic acid synthesis in Schizochytrium sp. H016 by exogenous supplementation of sesamol

Bao, Zhendong; Zhu, Yongming; Feng, Yue; Zhang, Kai; Zhang, Meng; Wang, Zhikuan; Yu, Liang

doi:10.1016/j.biortech.2021.126527

Cited by 19 publications

(7 citation statements)

References 48 publications

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“…The transcription level of APX gene in OMCM under PW condition was 3.24 times higher than that of WT under CW condition (Figure 5), which could prevent the DHA peroxidation. Moreover, exogenous antioxidants have become an effective means to increase DHA content of Schizochytrium sp., but endogenous carotenoids are more efficient 48,49 . It has been reported that higher level of ATP can be used as a driving force to increase terpenoids production in organisms 39,50 .…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, exogenous antioxidants have become an effective means to increase DHA content of Schizochytrium sp., but endogenous carotenoids are more efficient. 48,49 It has been reported that higher level of ATP can be used as a driving force to increase terpenoids production in organisms. 39,50 Here, the upregulation of MCM promoted more propionyl-CoA into TCA cycle, and the increased ATP not only promoted lipid accumulation but also carotenoids biosynthesis.…”

Section: Transcription Level Analysis Of Key Genesmentioning

confidence: 99%

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Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Zhang

et al. 2023

Biotechnology Journal

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In order to find a more effective way to obtain docosahexaenoic acid (DHA) rich lipid from Schizochytrium sp., a widespread propionate wastewater (PW) is used. PW is a common industrial and domestic wastewater, and transforming it into valuable products is a potential treatment method. Schizochytrium sp. is a rapidly growing oleaginous organism, which has been used commercially for DHA production. Herein, PW is completely used for DHA production by Schizochytrium sp. by genetic engineering and fermentation optimization, which can alleviate the increasingly tense demand for water resources and environmental pollution caused by industrial wastewater. Firstly, the methylmalonyl‐CoA mutase (MCM) was overexpressed in Schizochytrium sp. to enhance the metabolism of propionate, then the engineered strain of overexpressed MCM (OMCM) can effectively use propionate. Then, the effects of PW with different concentration of propionate were investigated, and results showed that OMCM can completely replace clean water with PW containing 5 g L−1 propionate. Furthermore, in the fed‐batch fermentation, the OMCM obtained the highest biomass of 113.4 g L−1 and lipid yield of 64.4 g L−1 in PW condition, which is 26.8% and 51.7% higher than that of wild type (WT) in PW condition. Moreover, to verify why overexpression of MCM can promote DHA and lipid accumulation, the comparative metabolomics, ATP production level, the antioxidant system, and the transcription of key genes were investigated. Results showed that ATP induced by PW condition could drive the synthesis of DHA, and remarkably improve the antioxidant capacity of cells by enhancing the carotenoids production. Therefore, PW can be used as an effective and economical substrate and water source for Schizochytrium sp. to accumulate biomass and DHA.

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Section: Resultsmentioning

confidence: 99%

Section: Transcription Level Analysis Of Key Genesmentioning

confidence: 99%

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Zhang

et al. 2023

Biotechnology Journal

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“…It has recently been shown that additional supplementation of Schizochytrium sp. H016 with antioxidant compounds such as sesamol can greatly reduce DHA loss from peroxidation, increasing DHA content in total fatty acids from 34.5% to 43.5% (Bao et al, 2022). Furthermore, the effects of phytohormones on lipid accumulation can be explored, as it has previously been demonstrated that gibberellin is able to increase DHA yield in thraustochytrid Aurantiochytrium sp.…”

Section: Metabolic Engineering Of Epa and Dha Production In Microalgaementioning

confidence: 99%

EPA and DHA in microalgae: Health benefits, biosynthesis, and metabolic engineering advances

Jesionowska,

Ovadia,

Hockemeyer

et al. 2023

J Americ Oil Chem Soc

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Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are ω‐3 very long‐chain polyunsaturated fatty acids (VLC‐PUFAs) that offer a wide range of human health benefits impacting cardiovascular, anti‐inflammatory, and neurological health. It is widely known that humans inefficiently synthesize these compounds and as such rely on exogenous dietary sources, such as marine fish oils. Unfortunately, the production of marine fish oils is an unsustainable process and has suffered a dramatic fall in recent years due to overfishing and climate change, as the demand for EPA and DHA continues to rise. Therefore, there is an urgent need to develop alternative, sustainable sources for consumable EPA and DHA. Metabolic engineering of marine microalgae to improve their EPA and DHA productivity is regarded as a promising option that has received increasing commercial attention in recent years. In this mini‐review, we describe several notable health benefits of EPA and DHA, summarize the natural sources and biosynthesis of VLC‐PUFAS, as well as the recent advances in metabolic engineering of EPA and DHA production in representative microalgal and protist species, including Schizochytrium sp., Phaeodactylum tricornutum, and Nannochloropsis oceanica.

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“…Recently, the role of adaptative lab evolution as a non-genetic approach to improve lipid (especially DHA) accumulation [ 11 ] and introduction of chemical modulators in the fermentation medium have been proposed to improve lipid yields [ 7 , 12 ]. Low-molecular-weight compounds such as propyl gallate [ 7 ], butanol [ 13 ], ascorbic acid [ 14 ], naphthoxyacetic acid (BNOA) [ 12 ] and phytohormones, such as kinetin (KIN), jasmonic acid (JA) and gibberellic acid (GA) [ 15 ], and sesamol [ 16 ], when added to the fermentation medium, could enhance lipid production with reference to polyunsaturated fatty acids (DHA, etc.). Such studies have led to enhancements in lipid production, but so far have not investigated the effects on fatty acid content and PUFA downstream processing, which is essential to produce SCO mimicking plant-like oil profiles suitable for food and pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Integration of the Exogenous Tuning of Thraustochytrid Fermentation and Sulfur Polymerization of Single-Cell Oil for Developing Plant-like Oils

et al. 2022

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In this study, we have demonstrated a bioprocessing approach encompassing the exogenous addition of low-molecular-weight compounds to tune the fatty acid (FA) profile in a novel thraustochytrid strain to produce desirable FAs. Maximum lipid recovery (38%, dry wt. biomass) was obtained at 1% Tween 80 and 0.25 mg/L of Vitamin B12. The transesterified lipid showed palmitic acid (C16, 35.7% TFA), stearic acid (C18, 2.1% TFA), and oleic acid (C18:1, 18.7% TFA) as the main components of total FAs, which are mainly present in plant oils. Strikingly, D-limonene addition in the fermentation medium repressed the production of polyunsaturated fatty acid (PUFAs). Sulfur-polymerization-guided lipid separation revealed the presence of saturated (SFAs, 53% TFA) and monounsaturated fatty acids (MUFAs, 46.6% TFA) in thraustochytrid oil that mimics plant-oil-like FA profiles. This work is industrially valuable and advocates the use of sulfur polymerization for preparation of plant-like oils through tuneable thraustochytrid lipids.

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Enhancement of lipid accumulation and docosahexaenoic acid synthesis in Schizochytrium sp. H016 by exogenous supplementation of sesamol

Cited by 19 publications

References 48 publications

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

EPA and DHA in microalgae: Health benefits, biosynthesis, and metabolic engineering advances

Integration of the Exogenous Tuning of Thraustochytrid Fermentation and Sulfur Polymerization of Single-Cell Oil for Developing Plant-like Oils

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