Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20

Tseng, Deng-Yu; Ho, Pei-Lin; Huang, Sung-Yan; Cheng, Sheng-Chi; Shiu, Ya‐Li; Chiu, Chiu‐Shia; Liu, Chun-Hung

doi:10.1016/j.fsi.2008.12.003

Cited by 250 publications

(173 citation statements)

References 24 publications

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“…There have been several positive reports on the use of probiotics in shrimp culture, such as improved balance of the intestinal microbiota (Li et al, 2009), production/stimulation of the production of digestive enzymes , improved growth rate , feed efficiency (Lin et al, 2004), immunostimulation (Chiu et al, 2007), resistance to infection by bacterial (Chiu et al, 2007) and viral pathogens (Tseng et al, 2009). However, the use of probiotics is still controversial due to some negative results (no action on the host) (Meunpol et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

Vinatea

2013

Pesq. agropec. bras.

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-The objective of this work was to isolate strains of lactic acid bacteria with probiotic potential from the digestive tract of marine shrimp (Litopenaeus vannamei), and to carry out in vitro selection based on multiple characters. The ideotype (ideal proposed strain) was defined by the highest averages for the traits maximum growth velocity, final count of viable cells, and inhibition halo against nine freshwater and marine pathogens, and by the lowest averages for the traits duplication time and resistance of strains to NaCl (1.5 and 3%), pH (6, 8, and 9), and biliary salts (5%). Mahalanobis distance (D 2 ) was estimated among the evaluated strains, and the best ones were those with the shortest distances to the ideotype. Ten bacterial strains were isolated and biochemically identified as Lactobacillus plantarum (3), L. brevis (3), Weissella confusa (2), Lactococcus lactis (1), and L. delbrueckii (1). Lactobacillus plantarum strains showed a wide spectrum of action and the largest inhibition halos against pathogens, both Gram-positive and negative, high growth rate, and tolerance to all evaluated parameters. In relation to ideotype, L. plantarum showed the lowest Mahalanobis (D 2 ) distance, followed by the strains of W. confusa, L. brevis, L. lactis, and L. delbrueckii. Among the analyzed bacterial strains, those of Lactobacillus plantarum have the greatest potential for use as a probiotic for marine shrimp.Index terms: Litopenaeus vannamei, acid lactic bacteria, bioprospection, pathogen inhibition. Seleção in vitro de bactérias com potencial para uso como probióticos na carcinicultura marinhaResumo -O objetivo deste trabalho foi isolar cepas de bactérias ácido-lácticas, com potencial probiótico do trato digestório de camarões marinhos e realizar seleção in vitro baseada em múltiplos caracteres. O ideótipo (cepa ideal proposta) foi definido por meio das maiores médias para os caracteres velocidade máxima de crescimento, contagem final de células viáveis e halo de inibição contra nove patógenos de origem continental e marinha, e pelas menores médias para os caracteres tempo de duplicação e tolerância das cepas a NaCl (1,5 e 3%), pH (6, 8 e 9) e sais biliares (5% Termos para indexação: Litopenaeus vannamei, bactérias ácido-lácticas, bioprospecção, inibição de patógenos.

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Section: Introductionmentioning

confidence: 99%

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

Vinatea

2013

Pesq. agropec. bras.

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“…to L. rohit [31], C. carpio [32] and Litopenaeus vannamei [33] was earlier found to significantly increase the phagocytic activity. 39 and 27% increase in phagocytic activity in grouper fish fed on B. pumilus and B. clausii supplemented diets respectively [23], 62% increase in O. niloticus fed on Micrococcus sps.…”

Section: Phagocytosismentioning

confidence: 88%

Effect of Dietary Supplementation of Bacillus subtilis and Terribacillus saccharophillus on Innate Immune Responses of a Tropical Freshwater Fish, Labeo rohita

Kalarani¹,

Sumathi²

2016

J Clin Cell Immunol

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Objective: Indiscriminate use of antibiotics to control fish diseases has led to the development and propagation of antibiotic resistant bacteria in aquatic ecosystems. Amongst the various alternatives proposed, enhancing immunity in fish using probiotics is the most widely accepted approach. Several earlier studies indicated the possibility of more potent effects of bacteria on fish when administered in live condition than as inactivated cells. But very few studies have explored the effect of gut bacteria on innate immune responses of fishes. Hence an attempt is made to study immunostimulatory effects of dietary supplementation of Bacillus subtilis and Terribacillus saccharophillus isolated from the gut of an edible Indian freshwater fish, Labeo rohita, in the same species.Methods: Long term administration (for 30 and 60 days) of B. subtilis or T. saccharophillus isolated from the gut wall of L. rohita were tested in the same species for their ability to improve innate immunity through measurement of the phagocytic activity, respiratory burst, myeloperoxidase activity, serum IgM levels, serum lectins, haemagglutination and haemolytic activity.Results: Dietary administration of 107 cfu/g of B.subtilis/T. saccharophillus for 30 (B 1 ) or 60 (B 2 ) days resulted in a significant increase in immune and humoral responses of L. rohita. Serum phagocytic activity, respiratory burst activity, myeloperoxidase activity, serum lectins, haemagglutination and haemolytic activity increased upon administration of B.subtilis / T. saccharophillus, except serum IgM levels which increased only in B 1 . A positive correlation was observed between the duration of supplementation of diets and the responses in both the cases. Conclusion:The results suggest that T. saccharophillus can also be used as a potent probiont similar to B.subtilis and both will have significant applications in fish feed formulations and disease prevention.

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“…Bacillus subtilis and Lactobacillus plantarum for Litopenaeus vannamei [55][56][57][58], Pediococcus acidilactici to Litopenaeus stylirostris [59] and Bacillus NL110 and Vibrio NE17 for Macrobrachium rosenberguii [60] also proved effective in improving the immune system of these animals.…”

Section: Immune Systemmentioning

confidence: 99%

Use of Probiotics in Aquaculture

Azevedo¹,

Braga²

2012

Probiotic in Animals

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Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20

Cited by 250 publications

References 24 publications

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

Effect of Dietary Supplementation of Bacillus subtilis and Terribacillus saccharophillus on Innate Immune Responses of a Tropical Freshwater Fish, Labeo rohita

Use of Probiotics in Aquaculture

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