Enhancement of glucose uptake in muscular cell by peptide fractions separated by electrodialysis with filtration membrane from salmon frame protein hydrolysate

Roblet, Cyril; Akhtar, Muhammad Javeed; Mikhaylin, Sergey; Pilon, Geneviève; Gill, Tom; Marette, André; Bazinet, Laurent

doi:10.1016/j.jff.2016.01.003

Cited by 54 publications

(43 citation statements)

References 23 publications

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“…We hypothesise that this is a result of migrating difficulties and polarity of high molecular weight peptides in an electric field. This explains the distribution of certain peptides in the KCL‐CP, since peptide or amino acids’ mobility depends on their size and charge (Roblet et al ., ). In contrast, Lys and Arg content was higher in KCL‐CP since they are strongly positively charged compared to His.…”

Section: Resultsmentioning

confidence: 97%

“…The separation of low molecular weight peptides was primarily the result of high positively charged to size ratio of such peptides, which easily migrate through the negative‐charged polyether sulfone ultrafiltration membrane (Suwal et al ., ). Similar results were obtained when separating peptides in the molecular mass range of 500–3000 Da from rainbow trout frame protein hydrolysates (Suwal et al ., ) and 300–800 Da from salmon frame protein hydrolysates (Roblet et al ., ). However, the bioactivities of peptides not only depend on molecular weight, but also on other variables such as amino acid composition and amino acid sequences in their chemical structure.…”

Section: Resultsmentioning

confidence: 97%

“…EDUF was also successful in recovery and concentration of bioactive (e.g. antioxidant, antihypertensive and antidiabetic) peptides from a variety of protein hydrolysates, including soybean, salmon frame, rapeseed, flaxseed and rainbow trout frames (Doyen et al ., ; Roblet et al ., , ; He et al ., ; Suwal et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Selective separation and characterisation of dual ACE and DPP‐IV inhibitory peptides from rainbow trout (Oncorhynchus mykiss) protein hydrolysates

Ketnawa

Suwal

Huang

et al. 2018

Int J of Food Sci Tech

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Microwave pretreatment and hydrolysis were applied to rainbow trout (Oncorhynchus mykiss) by-products to produce bioactive peptides with dual in vitro angiotensin-I converting enzyme (ACE) and dipeptidylpeptidase IV (DPP-IV) inhibitory activities. Peptides were fractionated using the single step electrodialysis with ultrafiltration membrane (EDUF). Concentration of cationic peptides (CP) increased in the recovery solution, reaching 125 lg mL À1 after a 4-h treatment with migration rate of 15.68 AE 2.98 g m À2 h. CP fractions displayed ACE and DPP-IV I inhibitory properties, with IC 50 values of 0.0036 mg mL À1 and 1.23 mg mL À1 respectively. The bioactivity was attributed to the low molecular weight peptides (300-500 Da) recovered. CP exhibited non-competitive inhibition patterns for ACE and DPP-IV, which were dose dependent. These results showed that bioactive peptides can successfully be separated from complex hydrolysate mixtures by EDUF. The fractionated peptides can serve as potential functional food ingredients or nutraceuticals for the management of hypertension and diabetes. Fractionation of active peptides by electrodialysis with ultrafiltration membraneA detailed description of the EDUF cell and operating conditions used for fractionation in this study is given in Suwal et al. (2018). Briefly, the cell configuration

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Selective separation and characterisation of dual ACE and DPP‐IV inhibitory peptides from rainbow trout (Oncorhynchus mykiss) protein hydrolysates

Ketnawa

Suwal

Huang

et al. 2018

Int J of Food Sci Tech

View full text Add to dashboard Cite

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“…In this context, Roblet et al (2016) have shown that peptides from salmon protein hydrolysates, separated by electrodialysis with filtration membrane, were found to enhance glucose uptake in L6 skeletal muscle cells. Similarly, LPIIDI and APGPAGP from Silver carp (Hypophthalmichthys molitrix Val.)…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo anti-diabetic and anti-hyperlipidemic effects of protein hydrolysates from Octopus vulgaris in alloxanic rats

Salem

Ktari

Bkhairia

et al. 2018

Food Research International

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This study aims to examine the effects of non-hydrolyzed octopus (Octopus vulgaris) muscle proteins (NHOPs) and their hydrolysates (OPHs) on alloxan induced diabetes in Wistar rats (AIDR). Animals were allocated into seven groups of six rats each: control group (C), diabetic group (D) and diabetic rats treated with acarbose (D + Acar), non-hydrolyzed octopus proteins (D + NHOPs) and octopus proteins hydrolysates (D + OPHs) groups. The diabetic rats presented a significant increase in glycemic status such as α-amylase activity (in plasma, pancreas and intestine), hepatic glycogen, blood glucose and glycated hemoglobin (HbA1c) levels, as well as a significant decrease in the levels of plasma insulin and total hemoglobin compared to control group. In addition, plasma and liver contents in total cholesterol, triglycerides and LDL-cholesterol significantly increased in AIDR compared to control group. However, the daily administration of OPHs for 30 days improved the glucose tolerance test, the glycemic status of diabetic rats and corrected the lipid profiles. Further, a significant increase in the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase as well as in the level of plasma bilirubin on diabetic status was observed, indicating considerable hepatocellular injury. OPHs treatment was found to attenuate the increased activities of the plasma enzymes produced by diabetes and caused a subsequent recovery towards normalization compared to the control group. By contrast, the NHOPs treatment was found to increase the glucose metabolic disorders in AIDR. These beneficial effects of OPHs were confirmed by histological findings in the hepatic and pancreatic tissues of diabetic treated rats. Indeed, they avoid lipid accumulation in the hepatocytes and protect the pancreatic β-cells from degeneration. Our results thus suggest that OPHs may be helpful in the preventing from diabetic complications by reversing hepatotoxicity.

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“…In order to recover fish protein by‐products and the release of bioactive peptides that benefits human health, several methods such as acid or alkaline hydrolysis, autolysis and enzymatic hydrolysis have been developed (Ghaly et al, ; Kim & Wijesekara, ). The enzymatic hydrolysis is the preferred technique to produce bioactive protein hydrolysates since it is a fast and controllable method in which several proteolytic enzymes are used to cleave the protein of the source, releasing amino acids and peptides with functional, nutritional and biological properties such as antioxidant, antihypertensive, antithrombotic, immunomodulatory and antimicrobial, among others (Abdelhedi et al, ; Ahn, Jeon, Kim, & Je, ; Centenaro et al, ; Ennaas, Hammami, Beaulieu, & Fliss, ; Hsu, Li‐Chan, & Jao, ; Intarasirisawat, Benjakul, Wu, & Visessanguan, ; Roblet et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary common carp by-product protein hydrolysates on antioxidant status in different organs of zebrafish (Danio rerio)

et al. 2018

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A feeding trial was conducted to evaluate the effect of dietary protein hydrolysates from common carp (Cyprinus carpio) by‐products on the antioxidant status of zebrafish (Danio rerio). Common carp by‐product was hydrolysed using Alcalase to a degree of hydrolysis of 15%. The zebrafish were fed for 44 days with four different diets with increasing levels of carp by‐product hydrolysates (CBH0: 0 g/kg; CBH25: 25 g/kg; CBH50: 50 g/kg; CBH100: 100 g/kg). The gills, muscle and brain were dissected at the end of the feeding trial in order to evaluate the total antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (TBARS). Although total antioxidant capacity did not show differences in muscle (p > 0.05), lipid peroxidation was reduced in the muscle of fish fed the CBH50 diet. Brain lipid peroxidation showed a significant reduction (p < 0.05) in all groups when compared with the control diet CBH0. Antioxidant properties of protein hydrolysates indicate their potential as nutraceuticals since (a) a reduction in muscle lipid peroxidation was verified, implying that their use could enhance the quality and shelf life of fish fillets; and (b) a decrease in brain lipid peroxidation was registered, highlighting the potential use of fish protein hydrolysates for the prevention of neurodegenerative diseases.

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Enhancement of glucose uptake in muscular cell by peptide fractions separated by electrodialysis with filtration membrane from salmon frame protein hydrolysate

Cited by 54 publications

References 23 publications

Selective separation and characterisation of dual ACE and DPP‐IV inhibitory peptides from rainbow trout (Oncorhynchus mykiss) protein hydrolysates

Selective separation and characterisation of dual ACE and DPP‐IV inhibitory peptides from rainbow trout (Oncorhynchus mykiss) protein hydrolysates

In vitro and in vivo anti-diabetic and anti-hyperlipidemic effects of protein hydrolysates from Octopus vulgaris in alloxanic rats

Effect of dietary common carp by-product protein hydrolysates on antioxidant status in different organs of zebrafish (Danio rerio)

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