Microwave pretreatment and hydrolysis were applied to rainbow trout (Oncorhynchus mykiss) by-products to produce bioactive peptides with dual in vitro angiotensin-I converting enzyme (ACE) and dipeptidylpeptidase IV (DPP-IV) inhibitory activities. Peptides were fractionated using the single step electrodialysis with ultrafiltration membrane (EDUF). Concentration of cationic peptides (CP) increased in the recovery solution, reaching 125 lg mL À1 after a 4-h treatment with migration rate of 15.68 AE 2.98 g m À2 h. CP fractions displayed ACE and DPP-IV I inhibitory properties, with IC 50 values of 0.0036 mg mL À1 and 1.23 mg mL À1 respectively. The bioactivity was attributed to the low molecular weight peptides (300-500 Da) recovered. CP exhibited non-competitive inhibition patterns for ACE and DPP-IV, which were dose dependent. These results showed that bioactive peptides can successfully be separated from complex hydrolysate mixtures by EDUF. The fractionated peptides can serve as potential functional food ingredients or nutraceuticals for the management of hypertension and diabetes.
Fractionation of active peptides by electrodialysis with ultrafiltration membraneA detailed description of the EDUF cell and operating conditions used for fractionation in this study is given in Suwal et al. (2018). Briefly, the cell configuration
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