Enhancement of glucose homeostasis through the PI3K/Akt signaling pathway by dietary with <i>Agaricus blazei</i> Murrill in STZ‐induced diabetic rats

Wei, Qi; Li, Jie; Zhan, Yuan; Zhong, Qiangui; Xie, Baogui; Chen, Lei; Chen, Bingzhi; Jiang, Yuji

doi:10.1002/fsn3.1397

Cited by 6 publications

(5 citation statements)

References 32 publications

(32 reference statements)

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“…Our findings showed that treatment with WEA (0.8 and 1.6 g/kg BW) induced a significant decrease in serum GLU and insulin levels, suggesting that reduction of serum GLU might not possibly be related to the reduced serum insulin levels in WEA-treated rats with NAFLD. The PI3K-Akt signaling pathway plays a potential role in the insulin signaling pathway, which is considered a key regulator of gluconeogenesis and glycogen synthesis [ 29 ]. Western blot analysis revealed that WEA administration reduced liver insulin resistance by increasing Akt phosphorylation at Ser473.…”

Section: Discussionmentioning

confidence: 99%

Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats

Deng

Liu

Tang

et al. 2022

BMC Complement Med Ther

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Background The “multiple-hit” hypothesis is currently the most widely accepted theory for non-alcoholic fatty liver disease (NAFLD) pathogenesis. The present study aimed to investigate the effects of the water extract of artichoke (WEA) on NAFLD and its underlying mechanism. Methods Rats were fed a high-fat diet (HFD) for 8 weeks to induce NAFLD and then treated with WEA at three doses (0.4, 0.8, and 1.6 g/kg body weight, BW) for 8 weeks. At the end of the intervention, serum biochemical parameters, hepatic antioxidant capacity, hepatic levels of pro-inflammatory cytokines, liver histopathology, hepatic inflammatory gene and lipid metabolism gene expression, and Akt and p-Akt (S473) protein levels were determined. Results The body weight, liver weight, liver triglyceride (TG) and serum levels of TG, total cholesterol, low-density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase, glucose, and insulin were all significantly reduced in the WEA-treated groups (0.8 and 1.6 g/kg BW) compared with the HFD group (P < 0.01). A significant decrease in hepatic content of malondialdehyde (P < 0.01) and glutathione (P < 0.01), as well as a significant increase in liver superoxide dismutase activity (P < 0.01) were observed in WEA-treated groups (0.8 and 1.6 g/kg BW) compared to the HFD group. In addition, there was a marked decrease in the hepatic levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the WEA-treated groups compared to the HFD group (P < 0.01). In line with these findings, the histopathology of the livers of rats treated with WEA (0.8 and 1.6 g/kg BW) showed a decrease in steatosis, ballooning, and lobular inflammation. Mechanistically, the reduced hepatic TG content might be related to the downregulation of lipogenic genes (SREBP1c, FASN, SCD1) and upregulation of lipolytic gene (PPARα), and the improved insulin signaling might be associated with the observed increase in antioxidant activity and reduction in inflammation in the WEA-treated groups. Conclusion The hepatoprotective role of WEA in NAFLD may be attributed to its anti-steatotic, antioxidant, anti-inflammatory, and anti-insulin resistance effects.

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Section: Discussionmentioning

confidence: 99%

Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats

Deng

Liu

Tang

et al. 2022

BMC Complement Med Ther

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“…Metabolomics is a large‐scale research technology that uses modern analytical method to assess the creature physiological status in different conditions (Wei et al., 2020 ). The metabolic profiles reflect an individual's function state at a certain point, which is consistent with the integrality and systematicness of traditional Chinese medicine (Bao et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics study of Angelica sinensis (Oliv.) Diels on the abnormal uterine bleeding rats by ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry analysis

Chen

Zou

Wang

et al. 2021

Food Science & Nutrition

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The objective of this study was to explore the effects and underlying intervention mechanisms of Angelica water extract (AWE) on abnormal uterine bleeding (AUB) based on serum metabolomics. Firstly, the concentration of main active substances in AWE was determined and the chemical components were identified by UPLC‐Q‐Exactive Orbitrap‐MS/MS. A drug‐induced abortion model was established by mifepristone and misoprostol. After administration AWE (2.16 g/kg) for 7 days, the coagulation function, serum hormone levels, H&E staining, and immunohistochemistry observation of uterus were detected. In addition, serum metabolites profiles were performed on ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS). The contents of ferulic acid, senkyunolide A, and ligustilide in AWE were 0.7276, 0.0868, and 1.9908 mg/g, respectively. Twenty‐six compounds were identified in AWE. It was found that AWE was effective in regulation of coagulation function and promoting endometrial recovery. Meanwhile, the levels of E2, Pg, and HCG and the expression of ERα, Erβ, and PR were down‐regulated in AUB model and up‐regulated by the treatment of AWE. Twenty‐one potential biomarkers were eventually identified by multivariate statistical analysis. Study indicated that glycerophospholipid, sphingolipid, amino acids, retinol metabolism and primary bile acid biosynthesis were the main related metabolic pathways involved for the treatment of AUB by AWE. The results showed that AWE has potential therapeutic effect on AUB by altering the metabolic aberrations.

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“…Binding to the ligand, EGFR forms a dimer and the phosphate of ATP transfers into the tyrosine residue. Then, different kinds of proteins bind to this phosphorylated tyrosine and signals transmit to downstream pathways, such as mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways [ 4 , 5 ]. It is well known that EGFR plays a key role in cell proliferation, apoptosis, and migration [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of 20(S)-Ginsenoside Rh2 as a Potential EGFR Tyrosine Kinase Inhibitor

Liang

Zhao

Zou

et al. 2022

Oxidative Medicine and Cellular Longevity

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As the main active ingredients of Panax ginseng, ginsenosides possess numerous bioactivities. Epidermal growth factor receptor (EGFR) was widely used as a valid target in anticancer therapy. Herein, the EGFR targeting activities of 20(S)-ginsenoside Rh2 (20(S)-Rh2) and the relationship of their structure-activity were investigated. Homogeneous time-resolved fluorescence assay showed that 20(S)-Rh2 significantly inhibited the activity against EGFR kinase. 20(S)-Rh2 was confirmed to effectively inhibited cell proliferation in a dose-dependent manner by MTT assay. Furthermore, quantitative real-time PCR and western blotting analysis revealed that 20(S)-Rh2 inhibited A549 cells growth via the EGFR-MAPK pathway. Meanwhile, 20(S)-Rh2 could promote cell apoptosis, block cell cycle, and reduce cell migration of A549 cells, respectively. In silico, the result suggested that both hydrophobic interactions and hydrogen-bonding interactions could contribute to stabilize their binding. Molecular dynamics simulation showed that the side chain sugar moiety of 20(S)-Rh2 was too flexible to be fixed at the active site of EGFR. Collectively, these findings suggested that 20(S)-Rh2 might serve as a potential EGFR tyrosine kinase inhibitor.

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Enhancement of glucose homeostasis through the PI3K/Akt signaling pathway by dietary with Agaricus blazei Murrill in STZ‐induced diabetic rats

Cited by 6 publications

References 32 publications

Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats

Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats

Metabolomics study of Angelica sinensis (Oliv.) Diels on the abnormal uterine bleeding rats by ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry analysis

Identification of 20(S)-Ginsenoside Rh2 as a Potential EGFR Tyrosine Kinase Inhibitor

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