Metabolomics study of Angelica sinensis (Oliv.) Diels on the abnormal uterine bleeding rats by ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry analysis

Abstract

The objective of this study was to explore the effects and underlying intervention mechanisms of Angelica water extract (AWE) on abnormal uterine bleeding (AUB) based on serum metabolomics. Firstly, the concentration of main active substances in AWE was determined and the chemical components were identified by UPLC‐Q‐Exactive Orbitrap‐MS/MS. A drug‐induced abortion model was established by mifepristone and misoprostol. After administration AWE (2.16 g/kg) for 7 days, the coagulation function, serum hormone levels, H&E staining, and immunohistochemistry observation of uterus were detected. In addition, serum metabolites profiles were performed on ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS). The contents of ferulic acid, senkyunolide A, and ligustilide in AWE were 0.7276, 0.0868, and 1.9908 mg/g, respectively. Twenty‐six compounds were identified in AWE. It was found that AWE was effective in regulation of coagulation function and promoting endometrial recovery. Meanwhile, the levels of E2, Pg, and HCG and the expression of ERα, Erβ, and PR were down‐regulated in AUB model and up‐regulated by the treatment of AWE. Twenty‐one potential biomarkers were eventually identified by multivariate statistical analysis. Study indicated that glycerophospholipid, sphingolipid, amino acids, retinol metabolism and primary bile acid biosynthesis were the main related metabolic pathways involved for the treatment of AUB by AWE. The results showed that AWE has potential therapeutic effect on AUB by altering the metabolic aberrations.

Detoxification technology and mechanism of processing with Angelicae sinensis radix in reducing the hepatotoxicity induced by rhizoma Dioscoreae bulbiferae in vivo

“…As the final product of lipid peroxidation, the content of MDA could reflect the damage degree of lipid peroxidation ( Jiang W. et al, 2021 ). T-SOD, GSH, and GPx are liver oxidation active enzymes, which could remove peroxides and free radicals, alleviate liver damage and protect hepatocytes ( Li et al, 2018 ; Xie et al, 2020b ; Li Z. et al, 2021 ; Yang et al, 2021 ), and were considered as important indicators reflecting hepatic oxidative damage ( Park et al, 2019 ; Chen T. et al, 2021 ). Consequently, after confirming that processing with ASR could significantly improve the liver toxicity induced by RDB, the potential molecular mechanism of ASR-processed RDB to reduce its liver toxicity was evaluated by detecting and analyzing Nrf2 and its downstream-related molecules.…”

Integrated Serum Pharmacochemistry, Metabolomics, and Network Pharmacology to Reveal the Material Basis and Mechanism of Danggui Shaoyao San in the Treatment of Primary Dysmenorrhea

“…As an effective preparation for the treatment of PD, the main components of DSS, such as chlorogenic acid, levistilide A, senkyunolide A, have shown good therapeutic effects in PD, uterine bleeding, and inflammatory activities ( Saltan et al, 2016 ; Chen et al, 2021 ; Zhang et al, 2021 ). Through the analysis of body weight, uterine organ index, and writhing behavior, it was found that the body weight of rats recovered to a certain extent, and the uterine index, writhing latency, and writhing times could be significantly improved after the continuous administration of DSS for 10 days.…”