Enhancement of Fe(III), Co(III), and Cr(VI) reduction at elevated temperatures and by a thermophilic bacterium

Zhang, C.; Shi, Lei; Logan, John M.; Mazumder, Raja; Phelps, Tommy J.

doi:10.1007/bf02941773

Cited by 54 publications

(47 citation statements)

References 18 publications

(11 reference statements)

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“…Cell enumeration was performed using fluorescence microscopy (Hobbie et al 1977). HClextractable Fe(II) was evaluated using ferrozine (Zhang et al 1996). Reduction of Co(III) to Co(II) was evaluated with a spectrophotometer by measuring absorbance at 535 nm (Zhang et al 1996).…”

Section: Methodsmentioning

confidence: 99%

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Metal reduction at cold temperatures by Shewanella isolates from various marine environments

Stapleton¹,

Sabree²,

Palumbo³

et al. 2005

Aquat. Microb. Ecol.

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Members of the genus Shewanella capable of reducing metals and forming minerals under cold-temperature conditions were isolated from 3 distinct marine habitats (the coast of Washington State, the Puget Sound, and an iron-rich microbial mat off Hawaii). Cultures of microorganisms were isolated at 8°C on nutrient agar medium prepared in artificial seawater. Isolates in this study could use a wide variety of electron acceptors such as oxygen, nitrate, and metals, and reduce various metals coupled to the oxidation of several organic acids, glucose or hydrogen at temperatures down to 0°C. Akaganeite was reduced to either magnetite or siderite, depending on the test conditions. The geochemical profiles at the sample sites from which these strains were isolated spanned a temperature range of 1.8 to 11°C, and all showed active oxygen and nitrate reduction as well as metal reduction. This confirms previous reports that sediment microorganisms participating in biogeochemical cycles remain active at low temperatures. KEY WORDS: Biogeochemistry · Geomicrobiology · Iron reduction · Cobalt reduction · Magneite Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 38: [81][82][83][84][85][86][87][88][89][90][91] 2005 While their quantitative importance in marine sediments and water columns is not well understood, members of the genus Shewanella are commonly encountered in marine environments (Perry et al. 1993, Thamdrup et al. 2000, Brettar et al. 2002. These isolates have provided useful models for laboratory investigations on metal reduction (Nealson & Saffarini 1994, Kostka & Nealson 1995, food spoilage, barophilic growth (Kato & Nogi 2001) and, more recently, psychrophilic enzymes (Irwin et al. 2001a,b). The present study describes the isolation of members of the genus Shewanella from 3 marine environments. Specifically, strains W3-6-1 and PS-7 were used as models to investigate microbial metal reduction and mineral formation at low temperatures. MATERIALS AND METHODSSite description and sampling. Marine sediment samples were collected from a transect off the coast of Washington State (USA), at 2 locations in Puget Sound (USA) and from an iron-rich microbial mat associated with the Naha Vents on the South Rift of Loihi Seamount near Hawaii. With the exception of the vent sites, sediments were collected by box core sampling. The microbial mat sample was collected during DSRV Pisces V dive number 342 (Emerson & Moyer 2002). Each box core was sub-cored for sectioning using 7.5 and 10 cm cast-acrylic core tubes. Core sectioning took place within approximately 1 h of coring in a glove-box at ~5°C in a N 2 atmosphere. Cores were stored at ~5°C to minimize effects due to warming. Details of sample location, including geographic coordinates, are listed in Table 1.The geochemistry of the sediments was inferred from sediment pore-water distributions. Pore-water was separated from the sediments by centrifugation at 7000 rpm (>10 000 × g) for 20 min; supernatants were filtered thr...

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Section: Methodsmentioning

confidence: 99%

“…HClextractable Fe(II) was evaluated using ferrozine (Zhang et al 1996). Reduction of Co(III) to Co(II) was evaluated with a spectrophotometer by measuring absorbance at 535 nm (Zhang et al 1996). Depletion of nitrate was measured using the Szechrome Reagent following manufacturer's instructions (Polysciences).…”

mentioning

confidence: 99%

Metal reduction at cold temperatures by Shewanella isolates from various marine environments

Stapleton¹,

Sabree²,

Palumbo³

et al. 2005

Aquat. Microb. Ecol.

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“…Bacterial numbers were determined by directly counting cells, which were stained with 0.1% acridine orange, under an epifluorescence microscope (57). A 0.1-ml sample of each dilution was inoculated into 28-ml pressure tubes containing 10 ml of basal medium and 0.05% yeast extract, with ferric citrate (10 mM) as the electron acceptor and hydrogen as the primary electron donor under a H 2 -CO 2 gas headspace.…”

Section: Methodsmentioning

confidence: 99%

“…To examine the chemical conditions of metal reduction and mineral formation by the thermophilic Fe(III)-reducing bacteria, subsamples (1 ml) of bacterial cultures and abiotic controls were taken from the culture bottles at different times and measured for redox potential (E h ) and pH at room temperature in an anaerobic chamber (58). Fe(II) concentrations (0.5 N HCl, soluble) were determined by measuring the absorbance at 562 nm on a Hewlett-Packard model 8453 spectrophotometer by the ferrozine method with anaerobic water for sample dilution (50,57).…”

Section: Methodsmentioning

confidence: 99%

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Isolation and Characterization of Metal-Reducing Thermoanaerobacter Strains from Deep Subsurface Environments of the Piceance Basin, Colorado

Roh

Liu

Huang

et al. 2002

Appl Environ Microbiol

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141

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Five bacterial strains were isolated from anaerobic enrichment cultures that had originated from inoculations with samples collected from the deep subsurface environments of the millions-of-years-old, geologically and hydrologically isolated Piceance Basin in Colorado. Small-subunit rRNA gene-based analyses indicated that all of these bacteria were closely related to Thermoanaerobacter ethanolicus, with similarities of 99.4 to 99.5%. Three isolates (X513, X514, and X561) from the five bacterial strains were used to examine physiological characteristics. These thermophilic bacteria were able to use acetate, glucose, hydrogen, lactate, pyruvate, succinate, and xylose as electron donors while reducing Fe(III), cobalt(III), chromium(VI), manganese(IV), and uranium(VI) at 60°C. One of the isolates (X514) was also able to utilize hydrogen as an electron donor for Fe(III) reduction. These bacteria exhibited diverse mineral precipitation capabilities, including the formation of magnetite (Fe 3 O 4 ), siderite (FeCO 3 ), rhodochrosite (MnCO 3 ), and uraninite (UO 2 ). The gas composition of the incubation headspace and the ionic composition of the incubation medium exerted profound influences on the types of minerals formed. The susceptibility of the thermophilic Fe(III)-reducing cultures to metabolic inhibitors specific for ferric reductase, hydrogenase, and electron transport indicated that iron reduction by these bacteria is an enzymatic process.

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Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium,Alkaliphilus metalliredigens (QYMF)

2007

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Enhancement of Fe(III), Co(III), and Cr(VI) reduction at elevated temperatures and by a thermophilic bacterium

Cited by 54 publications

References 18 publications

Metal reduction at cold temperatures by Shewanella isolates from various marine environments

Metal reduction at cold temperatures by Shewanella isolates from various marine environments

Isolation and Characterization of Metal-Reducing Thermoanaerobacter Strains from Deep Subsurface Environments of the Piceance Basin, Colorado

Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium,Alkaliphilus metalliredigens (QYMF)

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