Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium,Alkaliphilus metalliredigens (QYMF)

Roh, Yul; Chon, Chul-Min; Moon, Ji-Won

doi:10.1007/bf02857056

Cited by 45 publications

(23 citation statements)

References 29 publications

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“…Under anoxic conditions, vivianite is very stable (K sp = 10 À36 ; (Nriagu, 1972)) and can exert significant control over the geochemical cycles of Fe and P. Vivianite has also been reported as an end product of bacterial Fe(III) reduction Zachara et al, 1998;Roh et al, 2007;Peretyazhko et al, 2010). To our knowledge, the role of biogenic vivianite in abiotic uranium reduction and subsequent immobilization has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

Veeramani

Alessi

Suvorova

et al. 2011

Geochimica et Cosmochimica Acta

135

122

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Reductive immobilization of uranium by the stimulation of dissimilatory metal-reducing bacteria (DMRB) has been investigated as a remediation strategy for subsurface U(VI) contamination. In those environments, DMRB may utilize a variety of electron acceptors, such as ferric iron which can lead to the formation of reactive biogenic Fe(II) phases. These biogenic phases could potentially mediate abiotic U(VI) reduction. In this work, the DMRB Shewanella putrefaciens strain CN32 was used to synthesize two biogenic Fe(II)-bearing minerals: magnetite (a mixed Fe(II)-Fe(III) oxide) and vivianite (an Fe(II)-phosphate). Analysis of abiotic redox interactions between these biogenic minerals and U(VI) showed that both biogenic minerals reduced U(VI) completely. XAS analysis indicates significant differences in speciation of the reduced uranium after reaction with the two biogenic Fe(II)-bearing minerals. While biogenic magnetite favored the formation of structurally ordered, crystalline UO 2 , biogenic vivianite led to the formation of a monomeric U(IV) species lacking U-U associations in the corresponding EXAFS spectrum. To investigate the role of phosphate in the formation of monomeric U(IV) such as sorbed U(IV) species complexed by mineral surfaces, versus a U(IV) mineral, uranium was reduced by biogenic magnetite that was pre-sorbed with phosphate. XAS analysis of this sample also revealed the formation of monomeric U(IV) species suggesting that the presence of phosphate hinders formation of UO 2 . This work shows that U(VI) reduction products formed during in situ biostimulation can be influenced by the mineralogical and geochemical composition of the surrounding environment, as well as by the interfacial solute-solid chemistry of the solid-phase reductant.

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Section: Introductionmentioning

confidence: 99%

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

Veeramani

Alessi

Suvorova

et al. 2011

Geochimica et Cosmochimica Acta

135

122

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“…Natural hyperalkaline Ca 2ϩ -rich groundwaters formed by the serpentization of primary silicate materials olivine and pyroxene, have been studied for their microbial diversity (9). Furthermore, a select range of alkaliphiles capable of Fe(III) reduction have also been isolated from natural (10)(11)(12)(13) and anthropogenic (14,15) sites with pH values from 9.5 to 12.9. However, the impact of these microorganisms on materials and processes related to ILW remain essentially unknown.…”

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confidence: 99%

Microbial Reduction of Fe(III) under Alkaline Conditions Relevant to Geological Disposal

Williamson

Morris

Shaw

et al. 2013

Appl Environ Microbiol

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To determine whether biologically mediated Fe(III) reduction is possible under alkaline conditions in systems of relevance to geological disposal of radioactive wastes, a series of microcosm experiments was set up using hyperalkaline sediments (pH ϳ11.8) surrounding a legacy lime working site in Buxton, United Kingdom. The microcosms were incubated for 28 days and held at pH 10. There was clear evidence for anoxic microbial activity, with consumption of lactate (added as an electron donor) concomitant with the reduction of Fe(III) as ferrihydrite (added as the electron acceptor). The products of microbial Fe(III) reduction were black and magnetic, and a range of analyses, including X-ray diffraction, transmission electron microscopy, X-ray absorption spectroscopy, and X-ray magnetic circular dichroism confirmed the extensive formation of biomagnetite in this system. The addition of soluble exogenous and endogenous electron shuttles such as the humic analogue anthraquinone-2,6-disulfonate and riboflavin increased both the initial rate and the final extent of Fe(III) reduction in comparison to the nonamended experiments. In addition, a soluble humic acid (Aldrich) also increased both the rate and the extent of Fe(III) reduction. These results show that microbial Fe(III) reduction can occur in conditions relevant to a geological disposal facility containing cementbased wasteforms that has evolved into a high pH environment over prolonged periods of time (>100,000 years). The potential impact of such processes on the biogeochemistry of a geological disposal facility is discussed, including possible coupling to the redox conditions and solubility of key radionuclides.

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“…3, a serum "bottle A" that contained Fe(III) phase was exhibiting faint brownish yellow color, but its color changed to nearly colorless as shown in "bottle B". This reveals that Fe(III) phase was progressively reduced to a Fe(II) phase by bacteria (Roh et al, 2007). In that case, there almost not occurred a U(VI) decrease or reduction (Fig.…”

Section: Resultsmentioning

confidence: 81%

Influence of Iron Phases on Microbial U(VI) Reduction

Lee¹,

Baik²,

Lee³

et al. 2011

Journal of Soil and Groundwater Environment

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The bacterial uranium(VI) reduction and its resultant low solubility make this process an attractive option for removing U from groundwater. An impact of aqueous suspending iron phase, which is redox sensitive and ubiquitous in subsurface groundwater, on the U(VI) bioreduction by Shewanella putrefaciens CN32 was investigated. In our batch experiment, the U(VI) concentration (5×10 -5 M) gradually decreased to a non-detectable level during the microbial respiration. However, when Fe(III) phase was suspended in solution, bioreduction of U(VI) was significantly suppressed due to a preferred reduction of Fe(III) instead of U(VI). This shows that the suspending amorphous Fe(III) phase can be a strong inhibitor to the U(VI) bioreduction. On the contrary, when iron was present as a soluble Fe(II) in the solution, the U(VI) removal was largely enhanced. The microbially-catalyzed U(VI) reduction resulted in an accumulation of solid-type U particles in and around the cells. Electron elemental investigations for the precipitates show that some background cations such as Ca and P were favorably coprecipitated with U. This implies that aqueous U tends to be stabilized by complexing with Ca or P ions, which easily diffuse and coprecipitate with U in and around the microbial cell.

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Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium,Alkaliphilus metalliredigens (QYMF)

Cited by 45 publications

References 29 publications

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

Microbial Reduction of Fe(III) under Alkaline Conditions Relevant to Geological Disposal

Influence of Iron Phases on Microbial U(VI) Reduction

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