Enhancement of enzymatic hydrolysis of cellulose by surfactant

Abstract

Effects of surfactants on enzymatic saccharification of cellulose have been studied. Nonionic, amphoteric, and cationic surfactants enhanced the saccharification, while anionic surfactant did not. Cationic and anionic surfactants denatured cellulase in their relatively low concentrations, namely, more than 0.008 and 0.001%, respectively. Using nonionic surfactant Tween 20, which is most effective to the enhancement (e.g., the fractional conversion attained by 72 h saccharification of 5 wt % Avicel in the presence of 0.05 wt % Tween 20 is increased by 35%), actions of surfactant have been examined. As the results, it was suggested that Tween 20 plays an important role in the hydrolysis of crystalline cellulose and that Tween 20 disturbs the adsorption of endoglucanase on cellulose, i.e., varies the adsorption balance of endo- and exoglucanase, resulting in enhancing the reaction. The influence of Tween 20 to the saccharification was found to remain in simultaneous saccharification and fermentation of Avicel.

Ionic liquid‐mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis

“…Lignin is a major obstacle to enzyme attack on cellulose because it prevents enzyme accessibility (Chandra et al, 2007;Zhu et al, 2008) and can irreversibly adsorb cellulase (Ooshima et al, 1986). Indeed, our results confirm that a strong correlation exists between the fraction of cellulose hydrolyzed by cellulase and the fraction of lignin extracted from wood flour (Fig.…”

Cytogenetic Evaluation of the Effects of Chromium and Detergents in Vicia faba.

“…In this work the results were similar for both anionic and nonionic detergents, in spite of the findings of Ooshima et al (1986) that nonionic surfactants hydrolized cellulose by enzymatic saccharification to glucose while anionic surfactants did not.…”

Thermostable ß-D-Glucosidase from Aspergillus flavus: Production, Purification and Characterization

“…Similarly, higher activity of β-D-glucosidase of A. niger in the presence of detergents like Tween-80 and TritonX-100 was reported [49]. Several authors had proposed mechanisms to explain the saccharification of cellulose in the presence of surfactants [50,51,41]. The purified β-D-glucosidase was inhibited by detergents such as SDS, Tween 80 and Triton X-100 [52].…”