Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Wu, Kangping; Fang, Yilin; Hong, Biyuan; Cai, Yihui; Xie, Hua; Ruan, Roger; Cui, Xian; Yu, Zhigang; Liu, Yuhuan; Zhang, Qi

doi:10.3390/foods11172579

Cited by 4 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For several decades now, Saccharomyces cerevisiae and Escherichia coli have been representative industrial microorganisms with merits such as safety [16] and capability of modification by genetic engineering [17], resulting in their use as bio-converters for value-added production [18,19]. In recent years, not only these heterotrophic microorganisms but also autotrophic microorganisms including green algae such as Chlamydomonas reinhardtii and Chlorella vulgaris have attracted attention for use as material producers [20][21][22]. These green algal species can be additionally useful as bio-converters because these species can convert inorganic carbon sources such as carbon dioxide and organic carbon sources like acetic acid into useful metabolites [23].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Nakanishi,

Yomogita,

Horimoto

2024

Microorganisms

View full text Add to dashboard Cite

Utilities of whey powder (WP) and whey protein concentrate 34% powder (WPC34) prepared as dairy-processing residues were evaluated using a green alga Chlamydomonas reinhardtii. Analysis of C. reinhardtii growth showed that the strain used WP and WPC34 as nitrogen sources. Its specific growth rate and maximum cell density in WP-containing medium were higher than those in WPC34-containing medium; growth with WPC34 was improved by adding KCl or K2HPO4, which content was decreased as a result of WPC34’s preparation from WP. Although the lipid contents in media containing dairy-processing residues were 2.72 ± 0.31 wt% and 2.62 ± 0.20 wt% with no significant difference, the composition ratio of fatty acid C14 with WPC34 was higher than that with WP and the composition ratio of the sum of fatty acid-C16 and -C18 with WPC34 tended to be lower than that with WP. Additionally, analyses of gene transcription showed that the transcription level of acetyl-CoA carboxylase biotin carboxyl carrier protein in WPC34-containing medium was lower than that in WP-containing medium, possibly affecting the ratios of the chain lengths of fatty acids. The transcription of genes involved in glycolysis and the TCA cycle was outstandingly lower in algae grown in WPC34-containing medium when compared to those cultivated in the presence of WP, resulting in differences in energy production for cell proliferation.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Nakanishi,

Yomogita,

Horimoto

2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Chlorella in aquaculture: challenges, opportunities, and disease prevention for sustainable development

Aly

ElBanna²,

Fathi

2023

Aquacult Int

View full text Add to dashboard Cite

Aquaculture is the farming of aquatic organisms like fish, crustaceans, mollusks, and aquatic plants, which has become a crucial source of protein and income. However, bacterial infections pose a significant challenge to the aquaculture industry and traditional treatments, such as antibiotics and chemicals, have limitations and environmental concerns. Disease prevention and control measures, such as the use of probiotics, vaccines, and biosecurity measures, are essential for the sustainable development of the aquaculture industry. Further research is also needed to develop more effective and sustainable strategies for the prevention and control of bacterial fish pathogens in aquaculture, where alternative treatments such as herbal extracts, essential oils, and probiotics require further investigation for efficacy and safety. Microalgae, particularly Chlorella, have potential applications in various industries such as biofuels, pharmaceuticals, and wastewater treatment. However, their large-scale production and commercialization face challenges. Safety of Chlorella to fish is a crucial issue that requires careful evaluation, with hematology being an essential tool to assess its effects on fish health and physiology. Studies show that Chlorella is safe for fish and does not have adverse effects on growth, survival, or immune system function. Chlorella is a safe and sustainable option for aquaculture, free from harmful chemicals and antibiotics. The Green Water System utilizes Chlorella as a natural filter and nutrient recycler, improving water quality and providing a well-balanced diet for aquatic animals. This eco-friendly approach also enhances fish immune systems, growth rates, and survival rates. The scientometric review shows significant research activity, with Chang JS being a prominent author and People’s R China and the Chinese Academy of Sciences leading in contributions. The use of Chlorella shows promise as an alternative treatment for bacterial fish pathogens in aquaculture due to its antibacterial properties, safety, and sustainability. However, challenges such as cost-effectiveness and standardization need to be addressed for successful implementation in the aquaculture industry.

show abstract

Utilizing straw-derived hemicellulosic hydrolysates by Chlorella vulgaris: Contributing to a biorefinery approach

Kriechbaum,

Loaiza,

Friedl

et al. 2023

J Appl Phycol

View full text Add to dashboard Cite

With the growing human population, greenhouse gases and global temperature are increasing calling for sustainable biorefineries to convert low-value side streams into high-value products. In this study, the remaining hemicellulosic sugary waste fraction of a wheat straw biorefinery approach, without any pretreatment, was applied as a medium supplement for Chlorella vulgaris cultivation. Next to oligomeric and monomeric sugars, the waste stream contained hydrolysis by-products, such as furfural, 5-hydroxymethylfurfural and acetic acid. Despite the presence of these growth inhibitors, C. vulgaris cultivation was possible. Addition of 2.5% (v/v) of wheat straw hydrolysate to BG11-medium boosted chlorophyll content in C. vulgaris by 40% compared to photoautotrophically grown cells solely cultivated on defined media. As of now, this is the highest chlorophyll content obtained from a cultivation of C. vulgaris on a medium supplemented with a waste stream. Overall, our results demonstrate that wheat straw hydrolysate increases chlorophyll biosynthesis, improving the nutritional value of C. vulgaris biomass for food and feed applications. Hence, valorization of this hemicellulosic sugary fraction results in an additional high-valued product contributing towards the wheat straw biorefinery.

show abstract

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Cited by 4 publications

References 46 publications

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Chlorella in aquaculture: challenges, opportunities, and disease prevention for sustainable development

Utilizing straw-derived hemicellulosic hydrolysates by Chlorella vulgaris: Contributing to a biorefinery approach

Contact Info

Product

Resources

About