Enhancement of Carbazole Denitrogenation Rate Using Magnetically DecoratedBacillus clausiiBS1

Abstract

Bacillus clausii BS1 was coated by magnetic MFe 3 O 4 nanoparticles (MNPs), and transmission electron microscope analysis of the cells showed that the MNPs were efficiently assembled and adsorbed on the surface of the microbial cell. The coated cells showed higher biodenitrogenation (BDN) rate toward CAR than that of the free cells, recording complete removal and 94.25% removal of 1000 ppm CAR within seven days, with t 1/2 values of 31.36 and 64.78 h, respectively. Coated BS1 cells are characterized by higher BDN rate, storage and operational stabilities, and low sensitivity toward toxic by-products, and can be reused for four successive cycles without losing its biodenitrogenation efficiency and have the advantage of magnetic separation, which would resolve many operational problems in petroleum refinery.

Response of mixed bacterial culture towards dibenzothiophene desulfurization under the influence of surfactants and microscopically (SEM and TEM) characterized magnetic Fe3O4 nanoparticles

“…In contrast, the adsorption process is largely restricted by operational stability, biomass loading, biocatalytic activity, and strong adhesion, resulting from weak attachment forces between cells and adsorbent (Albayrak & Yang, 2002). The above issues could be managend by harnessing novel technology of remediating environmental hazards such as that coating the cells with magnetic particles (Zakaria et al, 2015). (Perrault & Chan, 2009; Wardani et al, 2019).…”