Enhancement of Bone Regeneration Using Osteogenic-Induced Adipose- Derived Stem Cells Combined with Demineralized Bone Matrix in a Rat Critically-Sized Calvarial Defect Model

Kim, Hyun Peel; Yi, Ji; Rhee, Seung Chul; Dhong, Eun Sang; Park, Seung Ha; Yoon, Eul Sik

doi:10.2174/157488812799859847

Cited by 32 publications

(14 citation statements)

References 21 publications

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“…Tables 1 and 2 present the main outcomes of preclinical and clinical studies that compile this review, respectively. In preclinical studies, fibrin glue loaded with MSCs was employed to regenerate bone defects in the femur [31,32,34,42], calvaria [33,37,39], maxilla and mandible [35,40,41], as well as to regenerate the cartilage of the knees [36,38]. Most studies used allogeneic MSCs, except for a few studies that opted for autologous MSCs [35,37,39].…”

Section: Resultsmentioning

confidence: 99%

Effects of Therapy with Fibrin Glue combined with Mesenchymal Stem Cells (MSCs) on Bone Regeneration: A Systematic Review

Ortiz

Pomini

Reis

et al. 2021

Cells

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Cell therapy strategies using mesenchymal stem cells (MSCs) carried in fibrin glue have shown promising results in regenerative medicine. MSCs are crucial for tissue healing because they have angiogenic, anti-apoptotic and immunomodulatory properties, in addition to the ability to differentiate into several specialized cell lines. Fibrin sealant or fibrin glue is a natural polymer involved in the coagulation process. Fibrin glue provides a temporary structure that favors angiogenesis, extracellular matrix deposition and cell-matrix interactions. Additionally, fibrin glue maintains the local and paracrine functions of MSCs, providing tissue regeneration through less invasive clinical procedures. Thus, the objective of this systematic review was to assess the potential of fibrin glue combined with MSCs in bone or cartilage regeneration. The bibliographic search was performed in the PubMed/MEDLINE, LILACS and Embase databases, using the descriptors (“fibrin sealant” OR “fibrin glue”) AND “stem cells” AND “bone regeneration”, considering articles published until 2021. In this case, 12 preclinical and five clinical studies were selected to compose this review, according to the eligibility criteria. In preclinical studies, fibrin glue loaded with MSCs, alone or associated with bone substitute, significantly favored bone defects regeneration compared to scaffold without cells. Similarly, fibrin glue loaded with MSCs presented considerable potential to regenerate joint cartilage injuries and multiple bone fractures, with significant improvement in clinical parameters and absence of postoperative complications. Therefore, there is clear evidence in the literature that fibrin glue loaded with MSCs, alone or combined with bone substitute, is a promising strategy for treating lesions in bone or cartilaginous tissue.

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Section: Resultsmentioning

confidence: 99%

Effects of Therapy with Fibrin Glue combined with Mesenchymal Stem Cells (MSCs) on Bone Regeneration: A Systematic Review

Ortiz

Pomini

Reis

et al. 2021

Cells

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“…Tissue engineering strategies for improved fracture healing have been expanding to include the use of MSCs derived from adipose tissue (adipose-derived stem cells, ASCs) due to the relative tissue abundance and potential to provide a large number of MSCs (Kim et al, 2012). Since differences exist among BMPs on cells well-documented to possess a high osteogenic capacity, a difference also likely exists among them for ASCs as well and may prove to be useful in maximizing their therapeutic potential.…”

Section: Introductionmentioning

confidence: 99%

Beyond osteogenesis: an in vitro comparison of the potentials of six bone morphogenetic proteins

Rivera¹,

Strohbach²,

Wenke³

et al. 2013

Front. Pharmacol.

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Bone morphogenetic proteins (BMPs) other than the clinically available BMP-2 and BMP-7 may be useful for improving fracture healing through both increasing osteogenesis and creating a favorable healing environment by altering cytokine release by endogenous cells. Given the spectrum of potential applications for BMPs, the objective of this study was to evaluate various BMPs under a variety of conditions to provide further insight into their therapeutic capabilities. The alkaline phosphatase (ALP) activity of both C2C12 and human adipose-derived stem cells (hASCs) was measured after exposure of increasing doses of recombinant human BMP-2, -4, -5, -6, -7, or -9 for 3 and 7 days. BMPs-2, -4, -5, -6, -7, and -9 were compared in terms of their ability to affect the release of stromal derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (b-FGF) from human bone marrow stromal cells (hBMSCs). Gene expression of ALP, osteocalcin, SDF-1, VEGF, and b-FGF following shRNA-mediated knockdown of BMP-2 and BMP-6 in hBMSCs or human osteoblasts under osteogenic differentiation conditions was also evaluated. Collectively, BMPs-6 and -9 produced the greatest osteogenic differentiation of C2C12 and hASCs as determined by ALP. The hBMSC secretion of SDF-1 was most affected by BMP-5, VEGF by BMP-4, and b-FGF by BMP-2. The knockdown of BMP-2 in BMSCs had no effect on any of the genes measured whereas BMP-6 knockdown in hBMSCs caused a significant increase in VEGF gene expression. BMP-2 and BMP-6 knockdown in human osteoblasts caused significant increases in VEGF gene expression and trends toward decreases in osteocalcin expression. These findings support efforts to study other BMPs as potential bone graft supplements, and to consider combined BMP delivery for promotion of multiple aspects of fracture healing.

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“…Having established the cell models, there have been numerous studies regarding cell therapies. For bone regeneration, a wide range of pluripotent stem cells have been studied in oral and maxillofacial surgery, human umbilical cord mesenchymal stem cells [200], bone marrow-derived stem cells [201], adipose-derived stem cells [202], mesenchymal dental pulp stem cells [203,204], periodontal ligament stem cells [205], and SHED [206]. While the wide use of these cells seems promising, strict vigilance and regulation is required to ensure standardized treatment outcomes.…”

Section: Cell Therapymentioning

confidence: 99%

Biomimetic Aspects of Oral and Dentofacial Regeneration

et al. 2020

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Biomimetic materials for hard and soft tissues have advanced in the fields of tissue engineering and regenerative medicine in dentistry. To examine these recent advances, we searched Medline (OVID) with the key terms “biomimetics”, “biomaterials”, and “biomimicry” combined with MeSH terms for “dentistry” and limited the date of publication between 2010–2020. Over 500 articles were obtained under clinical trials, randomized clinical trials, metanalysis, and systematic reviews developed in the past 10 years in three major areas of dentistry: restorative, orofacial surgery, and periodontics. Clinical studies and systematic reviews along with hand-searched preclinical studies as potential therapies have been included. They support the proof-of-concept that novel treatments are in the pipeline towards ground-breaking clinical therapies for orofacial bone regeneration, tooth regeneration, repair of the oral mucosa, periodontal tissue engineering, and dental implants. Biomimicry enhances the clinical outcomes and calls for an interdisciplinary approach integrating medicine, bioengineering, biotechnology, and computational sciences to advance the current research to clinics. We conclude that dentistry has come a long way apropos of regenerative medicine; still, there are vast avenues to endeavour, seeking inspiration from other facets in biomedical research.

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Enhancement of Bone Regeneration Using Osteogenic-Induced Adipose- Derived Stem Cells Combined with Demineralized Bone Matrix in a Rat Critically-Sized Calvarial Defect Model

Cited by 32 publications

References 21 publications

Effects of Therapy with Fibrin Glue combined with Mesenchymal Stem Cells (MSCs) on Bone Regeneration: A Systematic Review

Effects of Therapy with Fibrin Glue combined with Mesenchymal Stem Cells (MSCs) on Bone Regeneration: A Systematic Review

Beyond osteogenesis: an in vitro comparison of the potentials of six bone morphogenetic proteins

Biomimetic Aspects of Oral and Dentofacial Regeneration

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