Enhancement of biotransformation of ginsenosides in white ginseng roots by aerobic co-cultivation of Bacillus subtilis and Trichoderma reesei

Xie, Guangzhong; Guo, Bianqin; Li, Xiaomin; Liu, Shuai; Liu, Hongxia; Wang, Yongzhong

doi:10.1007/s00253-021-11631-1

Cited by 5 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salvia miltiorrhiza Bunge (Danshen) fermented by L. rhamnosus (F‐B4‐1) and B. subtilis Natto (F‐A7‐1) relieved dextran sulfate sodium‐induced ulcerative colitis in mice more effectively than raw Danshen [ 85 ]. Fermented white ginseng roots with B. subtilis and Trichoderma reesei enhanced the biotransformation yield from ginsenosides to rare ginsenosides, for these two species have nonsynchronous cell growth and different metabolic pathways [ 100 ]. The coimmobilized edible Aspergillus niger and yeast produced 11‐fold resveratrol from polydatin in Polygonum cuspidatum roots than that of the untreated sample [ 53 ].…”

Section: Probiotic Fermentation Modes For Chm Fermentationmentioning

confidence: 99%

“…The CHM bioactive natural product yield can be improved by optimization of synthetic fermentation microbiota and fermentation parameters (Figure 4D ). Fermentation with B. subtilis and T. reesei promoted biotransformation efficiency of ginsenosides in white ginseng roots, and the inoculation proportion of B. subtilis and T. reesei at 1:4 resulted in the highest rare ginsenoside yield [ 100 ]. Pretreatment of P. cuspidatum root with immobilized β ‐glucosidases could improve the conversion of polydatin to resveratrol at proper fermentation environment [ 140 ].…”

Section: Application Of Microbiome and Synthetic Biology Strategies F...mentioning

confidence: 99%

See 1 more Smart Citation

Research advances in probiotic fermentation of Chinese herbal medicines

Miao

Pan

Yin

et al. 2023

iMeta

View full text Add to dashboard Cite

Chinese herbal medicines (CHM) have been used to cure diseases for thousands of years. However, the bioactive ingredients of CHM are complex, and some CHM natural products cannot be directly absorbed by humans and animals. Moreover, the contents of most bioactive ingredients in CHM are low, and some natural products are toxic to humans and animals. Fermentation of CHM could enhance CHM bioactivities and decrease the potential toxicities. The compositions and functions of the microorganisms play essential roles in CHM fermentation, which can affect the fermentation metabolites and pharmaceutical activities of the final fermentation products. During CHM fermentation, probiotics not only increase the contents of bioactive natural products, but also are beneficial for the host gut microbiota and immune system. This review summarizes the advantages of fermentation of CHM using probiotics, fermentation techniques, probiotic strains, and future development for CHM fermentation. Cutting‐edge microbiome and synthetic biology tools would harness microbial cell factories to produce large amounts of bioactive natural products derived from CHM with low‐cost, which would help speed up modern CHM biomanufacturing.

show abstract

Section: Probiotic Fermentation Modes For Chm Fermentationmentioning

confidence: 99%

Section: Application Of Microbiome and Synthetic Biology Strategies F...mentioning

confidence: 99%

Research advances in probiotic fermentation of Chinese herbal medicines

Miao

Pan

Yin

et al. 2023

iMeta

View full text Add to dashboard Cite

show abstract

“…However, individual strains have limited effects in promoting the accumulation of medicinal components, and the combination of microbial agents has better promotion effects on the accumulation of ginsenosides. the production of ginsenosides in the co-culture mode of Bacillus subtilis and Trichoderma reesei was signi cantly higher than that in the mono-culture mode [33]. The combination of biological agents is an effective way to promote the accumulation of ginsenosides, but which agents are more effective in promoting the accumulation of ginsenosides needs to be studied, and its mechanism needs to be proved.…”

Section: Introductionmentioning

confidence: 99%

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Li,

Liu,

Gao

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: The ginseng endophyte Paenibacillus polymyxa Pp-7250 (Pp-7250) have multifaceted roles such as preventing ginseng diseases, promoting growth, increasing ginsenoside accumulation and degrading pesticide residues, these effects still room for improvements. Composite fungicide is an effective means to improve the biocontrol effect of fungicide, but the effect of Pp-7250 in combination with its symbiotic bacteria on ginseng needs to be further researched, and its mechanism of action still remains to be elucidated. In order to elucidate the effect of Paenibacillus polymyxa and Bacillus cereus co-bacterial agent on the yield and quality of understory ginseng, as well as to investigate its mechanism of action. Firstly, the effects of Bacillus cereus (BS), Paenibacillus polymyxa Pp-7250(PS) and Paenibacillus polymyxa and Bacillus cereus co-bacterial agent (PB) treatments on ginseng yield, ginsenoside content, disease prevention and pesticide degradation rate were evaluated by field experiments. Secondly, differences in microbial communities in ginseng rhizosphere soils with different treatments were analyzed using high‐throughput amplicon sequencing. Finally, correlation analysis was performed to elucidate the mechanism of action of PB co-bacterial agent in improving the yield and quality of ginseng. Results: The results indicate that PB treatment improved ginseng yield, ginsenoside accumulation, disease prevention and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, Trechisporaincreased and decreased the abundance of pathogenic microorganisms Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agent enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogen and animal pathogen. This study provided a theoretical reference for indepth research on improving the yield and quality of ginseng. Conclusion: The combined agent of Paenibacillus polymyxa and Bacillus cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease, improve the yield, quality and pesticide degradation.

show abstract

“…The application of Bacillus subtilis and Trichoderma reesei bacterial mixture improves the production of ginsenosides in ginseng compared to that with B. subtilis or T. reesei . [ 20 ]. The combination of biological agents is an effective way to promote the accumulation of ginsenosides.…”

Section: Introductionmentioning

confidence: 99%

Effects of a co-bacterial agent on the growth, disease control, and quality of ginseng based on rhizosphere microbial diversity

Li,

Liu,

Gao

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background The ginseng endophyte Paenibacillus polymyxa Pp-7250 (Pp-7250) has multifaceted roles such as preventing ginseng diseases, promoting growth, increasing ginsenoside accumulation, and degrading pesticide residues, however, these effects still have room for improvements. Composite fungicides are an effective means to improve the biocontrol effect of fungicides, but the effect of Pp-7250 in combination with its symbiotic bacteria on ginseng needs to be further investigated, and its mechanism of action has not been elucidated. In this study, a series of experiments was conducted to elucidate the effect of Paenibacillus polymyxa and Bacillus cereus co-bacterial agent on the yield and quality of understory ginseng, and to investigate their mechanism of action. Results The results indicated that P. polymyxa and B. cereus co-bacterial agent (PB) treatment improved ginseng yield, ginsenoside accumulation, disease prevention, and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms, including Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, and Trechispora, and decreased the abundance of pathogenic microorganisms, including Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agents enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, and terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogens and animal pathogens. Conclusion The combination of P. polymyxa and B. cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease and improve the yield, quality, and pesticide degradation.

show abstract

Enhancement of biotransformation of ginsenosides in white ginseng roots by aerobic co-cultivation of Bacillus subtilis and Trichoderma reesei

Cited by 5 publications

References 34 publications

Research advances in probiotic fermentation of Chinese herbal medicines

Research advances in probiotic fermentation of Chinese herbal medicines

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Effects of a co-bacterial agent on the growth, disease control, and quality of ginseng based on rhizosphere microbial diversity

Contact Info

Product

Resources

About