Enhancement of biosensing performance using a polyaniline/multiwalled carbon nanotubes nanocomposite

Hien, Hoang Thi Minh; Giang, Ho Truong; Trung, Tran; Tuấn, Chu Văn

doi:10.1007/s10853-016-0461-z

Cited by 20 publications

(10 citation statements)

References 30 publications

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“…Pure conducting polymers are formed in various structures such as nanowires, nanotubes and nano-thin films to obtain a large surface area and a high efficiency [5,6]. The combination of conducting polymers with dopants can provide additional advantages, such as high conductivity, large surface areas, environment-friendly features, stability and applicability in biosensors [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…These nanocomposites show advantageous properties of inorganic nanomaterials distributed in continuous polymer networks [14,15] and improvement of specifications of conducting polymers, including changes in electron structure of polymer chains, enhancement of charge transfer, and changes in conductivity of polymer chains [16,17]. Actually, several inorganic nanomaterials have been used to dope into the polymer networks, such as PANi/Ni [18], PANi/Au [19], PANi/WO3 [20], PANi/Mn2O3 [21], PANi/MnO2 [22] and PANi/MWCNTs [8] nanocomposites. In general, these nanocomposites can be synthesized by different techniques including chemical, electrochemical, photochemical and mechano-chemical methods and these developed materials have been applied for many applications such as energy storage, electrochemical biosensors, electrochemical sensors, FET-based biosensors, and coating and metal protecting [1].…”

Section: Introductionmentioning

confidence: 99%

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Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

Chu

Tran

et al. 2021

Preprint

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We describe in this report a development of label-free electrochemical DNA sensor based on a novel nanostructured electrode of multi-walled carbon nanotubes (MWCNTs)/ nano-flowers-like manganese dioxide (MnO2)/polyaniline nanowires (PANi NWs) nanocomposite. The nanocomposite was synthesized in-situ onto an interdigitated platinum microelectrode (Pt) using a combination of chemical and electrochemical synthesis methods: chemical preparation of MWCNTs/MnO2 and electropolymerization of PANi NWs. The fabricated MWCNTs/MnO2/PANi NWs was then used to develop a label-free electrochemical DNA sensor for a specific gene of Escherichia coli (E.coli) O157:H7 detection. The MWCNTs/MnO2/PANi NWs modified Pt electrode’s surface can facilitate for probe DNA strands immobilization and, therefore the electrochemical signal of the DNA sensors has been improved. The electrochemical impedance spectroscopy (EIS) measurements were conducted to investigate the output signals generated by the specific binding of probe and target DNA sequences. Obtained results indicated that the developed electrochemical biosensor can detect the target DNA in the linear range of 5 pM to 500 nM with a low limit of detection (LOD) at 4.42 × 10 –13 M. The research results demonstrated that the MWCNTs/MnO2/PANi NWs nanocomposite-based electrochemical DNA sensor has a great potential application to the development of highly sensitive and selective electrochemical DNA sensors to detect pathogenic agents.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

Chu

Tran

et al. 2021

Preprint

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“…The development of progressive diagnostic tools applicable for the determination of specific pathogenic flaviviruses in biological fluids is a very promising direction. In the last few decades, the researchers have shown particular interest in the development of electrochemical biosensors for the diagnosis of DENV, ZIKV, and JEV [25][26][27]. Electrochemical biosensors measure the intensity of the electrical signal provided by a special interaction with a target analyte and proportional to its concentration.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Khristunova

Dorozhko

Короткова

et al. 2020

Sensors

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A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.

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“…Electrochemical immunosensors utilizing inexpensive and more stable colloids of metal nanoparticles (NPs) for the labelling of immunoreagents can overcome the previously described disadvantages of ELISA and can thus be used as a suitable alternative. Recent publications confirm that there is an increased interest in the development of electrochemical immunological methods for TBE detection [7,8,9,10,11,12,13]. Table 1 summarizes types of electrodes, modifiers, and electrochemical labels applied for TBE determination.…”

Section: Introductionmentioning

confidence: 99%

“…From Table 1, it can be seen that in the majority of publications based on the determination of TBEV antigens, impedance electrochemical sensors with a sandwich format without labels were used [9,10,11,12,13]. In these works, the resistance at the electrode in the presence of redox probes before and after the formation of the antigen-antibody complex on the electrode surface is measured [9,10,11,12,13]. Most of them utilize a more complex and time- and labour-demanding technique for electrode modification.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

Khristunova

Короткова

Kratochvíl

et al. 2019

Sensors

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A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.

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Enhancement of biosensing performance using a polyaniline/multiwalled carbon nanotubes nanocomposite

Cited by 20 publications

References 30 publications

Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

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