Enhancement of antimicrobial and antitumor activities of zinc nanoparticles biosynthesized by Penicillium chrysogenum AUMC 10608 using gamma radiation

Abstract

A N ECO-FRIENDLY protocol for the biosynthesis of extracellular zinc nanoparticles (ZnNPs) by Penicillium chrysogenum was performed. The formation of ZnNPs was observed by the formation of white aggregates of ZnNPs and confirmed by UV absorption spectrum at 340nm. The synthesized ZnNPs were also characterized by High resolutiontransmission electron microscope (HR-TEM), dynamic light scattering (DLS) and fourier transform infrared spectroscopy (FT-IR). The optimum conditions were pH 10, 72h of reaction between culture filtrate (CF) and 1mM (ZnSO 4) solution with (6:1) ratio. Exposure of ZnNPs to gamma radiation showed an increase in the absorption band intensities when compared to the non irradiated sample. Samples of ZnNPs exposed to 20kGy dose recorded the greatest antimicrobial effect. The study of its antitumor activity against human breast carcinoma (MCF-7) and colon carcinoma cells (HCT-116) was performed. The IC 50 values of (MCF-7) were 373µg/ml and >500µg/ml, while of (HCT-116) were 226 and 317µg/ml for irradiated and non irradiated samples, respectively. TEM and DLS showed the formation of spherical monodispersed ZnNPs from 9 to 17nm with a mean of 13nm for the irradiated sample exposed to 20kGy. The current research provided an insight on using ɤ-radiation as a highly efficient and inexpensive tool for the enhancement of antimicrobial and antitumor effects of nanoparticles against infectious and tumor diseases.

ZnO Nanoparticle-Mediated Seed Priming Induces Biochemical and Antioxidant Changes in Chickpea to Alleviate Fusarium Wilt

“…Moreover, the difference in the shape of absorbance peaks of zinc oxide and filtrate represented the reduction of zinc acetate salt into ZnO NPs. A similar peak of absorption for zinc oxide at 340 nm has been reported earlier [ 32 ].…”

An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum

“…Recently, Penicillium chrysogenum strains have been used as a biocatalyst for the green synthesis of a wide range of metal and metal oxide NPs [ 33 , 34 ]. These activities could be attributed to the efficacy of P. chrysogenum to produce novel active metabolites which are utilized for reducing, capping, and stabilizing metal and metal oxide NPs [ 35 , 36 ].…”

Microbial Mediated Synthesis of Zinc Oxide Nanoparticles, Characterization and Multifaceted Applications

“…The anticancer activity of ZnONPs in different cancer types is presented in Table 3. The study of Housseiny and Gomaa [119] provided an insight on using ɤ-radiation as a highly efficient and inexpensive tool for the enhancement of antitumor effects of NPs against tumor diseases. Samples of ZnONPs produced by Penicillium chrysogenum exposed to 20 kGy dose recorded the greatest antitumor effect.…”

“…Rhodococcus pyridinivorans HT-29, colon ZnONPs suppressed cell viability in Caco-2 cell line via increased ROS and induced IL-8 release ZnO NPs and fatty acids could induce lysosomal destabilization in Caco-2 cells [189,207,208] Aspergillus niger HepG2, liver ZnONPs caused ROS generation and oxidative DNA damage and lead to mitochondrial-mediated apoptosis in HepG2 cells ZnONPs selectively induce apoptosis in HepG2 cells, which was also mediated by ROS via the p53 pathway [28,209,210] Aspergillus niger A549, lung ZnONPs incorporated in liposomes not only rendered pH responsivity to the delivery carrier but also exhibited synergetic chemo-photodynamic anticancer action [211,212] Aspergillus terreus MCF-7, breast Ecofriendly formulated ZnONPs arrest the cell cycle in the G2/M phase and upregulated proapoptotic genes p53, p21, Bax, and JNK and downregulated antiapoptotic genes Bcl-2, AKT1, and ERK1/2 in a dose-dependent manner in MCF-7 cells [213] Penicillium chrysogenum MCF-7, breast HCT-116, colon A doxorubicin delivery system based on zinc oxide nanomaterials can bypass the P-gp increase in the drug accumulation in resistant MCF-7R and MCF-7S cells ZnO NPs induced Caco-2 cells cytotoxicity associated with increased intracellular Zn ions [119,214,215] Pichia kudriavzevii MCF-7, breast RGD (Arg-Gly-Asp)-targeted ZnO NPs can target integrin αvβ3 receptors to increase the toxicity of the ZnO NPs to MDA-MB-231 cells at lower doses [216] similar study, the cotton wound bandages impregnated with ZnONPs give patches with antimicrobial properties. This property can potentially be used for treating and covering infection-sensitive wounds, namely diabetic or burn wounds [89].…”