Enhancement of angiogenesis and neurogenesis by intracerebroventricular injection of secretome from human embryonic stem cell‐derived mesenchymal stem cells in ischemic stroke model

Taei, Afsaneh Asgari; Nasoohi, Sanaz; Hassanzadeh, Gholamreza; Kadivar, Mehdi; Dargahi, Leila; Farahmandfar, Maryam

doi:10.1016/j.biopha.2021.111709

Cited by 47 publications

(25 citation statements)

References 64 publications

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“…Furthermore, it is reported that transplanted MSCs could promote the neuroprotective effect after stroke by promoting angiogenesis and neurogenesis via paracrine secretion of an array of bioactive factors and neurotrophins 36,37 . To gain a further insight into the MSCs‐mediated neuronal protection, herein, the endothelial cells of microvessels and neuroblasts origin were stained by CD31 and DCX, respectively, to explore the reparative effects of CPSP‐labeled MSCs on neuroprotective functions.…”

Section: Resultsmentioning

confidence: 99%

Metal protoporphyrin‐induced self‐assembly nanoprobe enabling precise tracking and antioxidant protection of stem cells for ischemic stroke therapy

et al. 2023

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Mesenchymal stem cell (MSC)‐based therapy has provided a promising strategy for the treatment of ischemic stroke, which is still restricted by the lack of long‐term cell tracking strategy as well as the poor survival rate of stem cells in ischemic region. Herein, a dual‐functional nanoprobe, cobalt protoporphyrin‐induced nano‐self‐assembly (CPSP), has been developed through a cobalt protoporphyrin IX (CoPP) aggregation‐induced self‐assembly strategy, which combines CoPP and superparamagnetic iron oxide (SPION) via a simple solvent evaporation‐driven method. Without any additional carrier materials, the obtained CPSP is featured with good biocompatibility and high proportions of active ingredients. The SPIONs in CPSPs form a cluster‐like structure, endowing this nano‐self‐assembly with excellent T2‐weighted magnetic resonance (MR) imaging performance. Furthermore, the CoPP released from CPSPs could effectively protect MSCs by upregulating heme oxygenase 1 (HO‐1) expression. The in vivo cell tracing capacity of CPSPs is confirmed by monitoring the migration of labeled MSCs with MR imaging in a middle cerebral artery occlusion mouse model. More importantly, the sustained release of CoPP from CPSPs improves the survival of transplanted MSCs and promotes neural repair and neurobehavioral recovery of ischemic mice. Overall, this work presents a novel dual‐functional nanoagent with an ingenious design for advancing MSC‐based therapy.

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Section: Resultsmentioning

confidence: 99%

Metal protoporphyrin‐induced self‐assembly nanoprobe enabling precise tracking and antioxidant protection of stem cells for ischemic stroke therapy

et al. 2023

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“…Secondly, the transplanted stem cells alter the injured environment via the cytokines secreted. The secreted cytokines might possess the effects of neuroprotection [ 12 , 39 , 40 , 41 ], neuroregeneration [ 42 , 43 ], angiogenesis [ 12 , 41 , 42 , 43 , 44 ], or immunomodulation [ 39 , 45 , 46 , 47 ], and thereby repair the damaged tissues or prevent them from further deterioration. From the RT-PCR results of our study, it is suggested that HUMSCs did not directly differentiate into neurons or astrocytes after transplantation into the infarcted cortex of the chronic stroke rats.…”

Section: Discussionmentioning

confidence: 99%

Xenograft of Human Umbilical Mesenchymal Stem Cells Promotes Recovery from Chronic Ischemic Stroke in Rats

Yeh

Chu

et al. 2022

IJMS

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Stroke is a leading cause of adult disability. In our previous study, transplantation of human umbilical mesenchymal stem cells (HUMSCs) in Wharton’s jelly in the acute phase of ischemic stroke promotes recovery in rats. Unfortunately, there is no cure for chronic stroke. Patients with chronic stroke can only be treated with rehabilitation or supportive interventions. This study aimed to investigate the potential of xenograft of HUMSCs for treating chronic stroke in rats. Rats were subjected to 90 min middle cerebral artery occlusion and then reperfusion to mimic ischemic cerebral stroke. On day 14 following stroke, HUMSCs were transplanted into the damaged cerebral cortex. The motor function in rats of the Stroke + HUMSCs group exhibited significant improvement compared to that of the Stroke + Saline group, and the trend persisted until day 56 post stroke. The cerebral cortex changes were tracked using magnetic resonance imaging, showing that cerebral atrophy was found starting on day 7 and was reduced significantly in rats receiving HUMSCs compared to that in the Stroke + Saline group from day 21 to day 56. HUMSCs were found to be existed in the rats’ cerebral cortex on day 56, with signs of migration. The grafted HUMSCs did not differentiate into neurons or astrocytes and may release cytokines to improve neuroprotection, decrease inflammation and increase angiogenesis. Our results demonstrate that xeno-transplantation of HUMSCs has therapeutic benefits for chronic ischemic stroke. Most importantly, patients do not need to use their own HUMSCs, which is a gospel thing for clinical patients.

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“…The cylinder test was conducted to evaluate the motor function and asymmetry of forelimb usage in post-ischemic rats ( Asgari Taei et al, 2021 ). The rats were placed in a Plexiglas cylinder (diameter 20 cm, height 30 cm) on a clean desktop, and forelimb use was observed for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Acupuncture at GV20 and ST36 Improves the Recovery of Behavioral Activity in Rats Subjected to Cerebral Ischemia/Reperfusion Injury

Yang

Deng

et al. 2022

Front. Behav. Neurosci.

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Traditional acupuncture and electroacupuncture (EA) have been widely performed to treat ischemic stroke. To provide experimental support for the clinical application of acupuncture to ameliorate post-stroke sequelae, in this study, we investigated the therapeutic effect of acupuncture and EA on CIRI following middle cerebral artery occlusion (MCAO) in rats. The animals were randomly divided into five groups: sham-operated (S), model (M), traditional acupuncture (A) treatment, electroacupuncture (EA) treatment, and drug (D; edaravone) therapies. Neurological behavioral characteristics (neurological deficit score, forelimb muscle strength, sensorimotor function, body symmetry, sucrose consumption, and mood) were examined in all the groups on days 1, 3, 5, and 7 after reperfusion. Expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) were detected by immunohistochemistry. Both acupuncture and EA significantly reduced neurological deficits and improved forelimb muscle strength, sensorimotor function, body symmetry recovery, and neurovascular regeneration in the rats after ischemia/reperfusion injury. The efficacies of both acupuncture and EA were comparable to that of edaravone, a commonly used medicine for stroke in the clinic. Thus, our data suggest that acupuncture and EA therapy at acupoints GV20 and ST36 might represent alternative or complementary treatments to the conventional management of ischemic stroke, providing additional support for the experimental evidence for acupuncture therapy in clinical settings. In summary, EA might provide alternative or complementary treatment strategies for treating patients with apoplexy in the clinic. However, potential mechanisms underlying the role of acupuncture require further investigation.

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Enhancement of angiogenesis and neurogenesis by intracerebroventricular injection of secretome from human embryonic stem cell‐derived mesenchymal stem cells in ischemic stroke model

Cited by 47 publications

References 64 publications

Metal protoporphyrin‐induced self‐assembly nanoprobe enabling precise tracking and antioxidant protection of stem cells for ischemic stroke therapy

Metal protoporphyrin‐induced self‐assembly nanoprobe enabling precise tracking and antioxidant protection of stem cells for ischemic stroke therapy

Xenograft of Human Umbilical Mesenchymal Stem Cells Promotes Recovery from Chronic Ischemic Stroke in Rats

Acupuncture at GV20 and ST36 Improves the Recovery of Behavioral Activity in Rats Subjected to Cerebral Ischemia/Reperfusion Injury

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