Acupuncture at GV20 and ST36 Improves the Recovery of Behavioral Activity in Rats Subjected to Cerebral Ischemia/Reperfusion Injury

Yang, Yang; Deng, Peiying; Si, Ying-kui; Xu, Hong; Zhang, Jianmin; Sun, Hua

doi:10.3389/fnbeh.2022.909512

Cited by 16 publications

(11 citation statements)

References 50 publications

(54 reference statements)

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“…Several studies have proven the significant neuroprotective effect of the widely used acupoints ST36 and GV20 in the management of neurological diseases 35–37 . Specifically, the ST36 has proven to be beneficial in alleviating chronic pain 38 .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have proven the significant neuroprotective effect of the widely used acupoints ST36 and GV20 in the management of neurological diseases. 35 , 36 , 37 Specifically, the ST36 has proven to be beneficial in alleviating chronic pain. 38 Consistent with previous findings, we found that EA stimulation acupoints at ST36 and GV20 markedly decreased pain hypersensitization induced by CCI surgery, with the anti‐nociceptive effect increased in a time‐dependent manner.…”

Section: Discussionmentioning

confidence: 99%

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Electroacupuncture suppresses neuronal ferroptosis to relieve chronic neuropathic pain

Xue,

Kui,

Huang

et al. 2024

J Cellular Molecular Medi

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Growing evidence supports the analgesic efficacy of electroacupuncture (EA) in managing chronic neuropathic pain (NP) in both patients and NP models induced by peripheral nerve injury. However, the underlying mechanisms remain incompletely understood. Ferroptosis, a novel form of programmed cell death, has been found to be activated during NP development, while EA has shown potential in promoting neurological recovery following acute cerebral injury by targeting ferroptosis. In this study, to investigate the detailed mechanism underlying EA intervention on NP, male Sprague‐Dawley rats with chronic constriction injury (CCI)‐induced NP model received EA treatment at acupoints ST36 and GV20 for 14 days. Results demonstrated that EA effectively attenuated CCI‐induced pain hypersensitivity and mitigated neuron damage and loss in the spinal cord of NP rats. Moreover, EA reversed the oxidative stress‐mediated spinal ferroptosis phenotype by upregulating reduced expression of xCT, glutathione peroxidase 4 (GPX4), ferritin heavy chain (FTH1) and superoxide dismutase (SOD) levels, and downregulating increased expression of acyl‐CoA synthetase long‐chain family member 4 (ACSL4), malondialdehyde levels and iron overload. Furthermore, EA increased the immunofluorescence co‐staining of GPX4 in neurons cells of the spinal cord of CCI rats. Mechanistic analysis unveiled that the inhibition of antioxidant pathway of Nrf2 signalling via its specific inhibitor, ML385, significantly countered EA's protective effect against neuronal ferroptosis in NP rats while marginally diminishing its analgesic effect. These findings suggest that EA treatment at acupoints ST36 and GV20 may protect against NP by inhibiting neuronal ferroptosis in the spinal cord, partially through the activation of Nrf2 signalling.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Electroacupuncture suppresses neuronal ferroptosis to relieve chronic neuropathic pain

Xue,

Kui,

Huang

et al. 2024

J Cellular Molecular Medi

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“…Skeletal muscle is the main target of Adipo and a major location of AdipoR1. Although skeletal muscle is considered to be one of the targets of pharmacological treatment for IR or diabetes ( Dong et al, 2021 ; Wei et al, 2021 ; Yu et al, 2022 ), some studies have shown that acupuncture ST36 may stimulate skeletal muscle and improve the recovery of behavioral activity in rats subjected to cerebral ischemia or reperfusion injury ( Yang et al, 2022 ). Sun demonstrated that acupuncture at ST36 increased motor cortical excitation ( Sun et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Activation of POMC neurons to adiponectin participating in EA-mediated improvement of high-fat diet IR mice

Chang

et al. 2023

Front. Neurosci.

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BackgroundInsulin resistance (IR) is one of the common pathological manifestations of metabolic-related diseases, and the prevalence of relevant diseases is high. Acupuncture is beneficial to IR patients, but the central mechanism underlying this treatment remains unclear. This study provides mechanistic insights into how electroacupuncture (EA) improves IR through the response of Pro-opiomelanocortin (POMC) neurons to adiponectin (Adipo).MethodsGlucose tolerance tests (GTT), Insulin tolerance tests (ITT) and fasting blood glucose (FBG) were detected by glucometer. Serum insulin, Adipo and skeletal muscle adiponectin receptor 1 (AdipoR1) protein levels were examined by ELISA. Homeostasis model assessment estimated insulin resistance (HOMA-IR) was calculated using the following formula: HOMA-IR = fasting insulin (FINS) (mU/L) × FBG (mmol/L)/22.5. The expression levels of AdipoR1 and Adipo mRNA in skeletal muscle were detected by real-time PCR quantification. The co-marking of c-Fos/AdipoR1 and POMC neurons were investigated using immunofluorescence. Spontaneous excitatory postsynaptic currents (sEPSCs) of POMC neurons and the response of POMC neurons to Adipo were detected via electrophysiology.ResultsEA significantly ameliorated HFD-induced impairment of GTT, ITT, FBG, and HOMA-IR which was correlated with recovery of the expression level of AdipoR1 and Adipo in skeletal muscle. The improved response of POMC neurons to Adipo in the hypothalamus may be a key factor in correcting abnormal glucose tolerance and improving IR.ConclusionThis study demonstrates that EA can ameliorate HFD-induced impaired glucose tolerance through improved response of POMC neurons to Adipo in the hypothalamus, providing insight into the central mechanism of improving IR through EA.

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“…Numerous clinical and basic studies have shown that electroacupuncture (EA) at Baihui (GV20) and Zusanli (ST36) is effective in treating cerebral ischemia, but its mechanism is still unclear [ 11 , 12 ]. At present, there has been no research report on the neuroprotective effect of EA on pMCAO secondary hypothalamic injury by regulating the 5-HT/5-HT2A signal pathway.…”

Section: Introductionmentioning

confidence: 99%

Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia

Nie

et al. 2023

CNR

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Background: Previous studies have shown that the neurological damage caused by middle cerebral artery occlusion (MCAO) is not only limited to local infarction but can also cause secondary damage in distant sites, such as the hypothalamus. 5-hydroxytryptamine (5-HT)/ 5-HT transporter (5-HTT) and 5-HT receptor 2A (5-HT2A) are important in the treatment of cerebrovascular diseases. Objective: This study aimed to study the effect of electroacupuncture (EA) on the expression of 5-HT, 5-HTT, and 5-HT2A in the hypothalamus of rats with ischemic brain injury and to explore the protective effect and potential mechanism of EA on the secondary injury of cerebral ischemia. Methods: Sprague-Dawley (SD) rats were randomly divided into three groups: sham group, model group, and EA group. The permanent middle cerebral artery occlusion (pMCAO) method was used to induce ischemic stroke in rats. In the EA group, the Baihui (GV20) and Zusanli (ST36) points were selected for treatment, which was administered once per day for two consecutive weeks. The neuroprotective effect of EA was evaluated by nerve defect function scores and Nissl staining. The content of 5-HT in hypothalamus was detected by enzyme linked immunosorbent assay (ELISA), and the expression of 5-HTT and 5-HT2A were detected by Western blot. Results: Compared with that in the sham group, the nerve defect function score in the model group rats was significantly increased, the hypothalamus tissue showed obvious nerve damage, the levels of 5-HT and the expression of 5-HTT were significantly reduced, and the expression of 5-HT2A was significantly increased. After 2 weeks of EA treatment, the nerve defect function scores of pMCAO rats were significantly reduced, the hypothalamic nerve injury was significantly reduced, the levels of 5-HT and the expression of 5-HTT were significantly increased, and the expression of 5-HT2A was significantly decreased. Conclusion: EA has a certain therapeutic effect on hypothalamic injury secondary to permanent cerebral ischemia, and its potential mechanism may be closely related to the upregulation of 5-HT and 5-HTT expression and the downregulation of 5-HT2A expression.

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Acupuncture at GV20 and ST36 Improves the Recovery of Behavioral Activity in Rats Subjected to Cerebral Ischemia/Reperfusion Injury

Cited by 16 publications

References 50 publications

Electroacupuncture suppresses neuronal ferroptosis to relieve chronic neuropathic pain

Electroacupuncture suppresses neuronal ferroptosis to relieve chronic neuropathic pain

Activation of POMC neurons to adiponectin participating in EA-mediated improvement of high-fat diet IR mice

Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia

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