Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis

Костылева, Е.В.; Tsurikova, N. V.; Середа, А. С.; Velikoretskaya, I.A.; Веселкина, Т. Н.; Lobanov, N. S.; Шашков, И. А.; Sinitsyn, A. P.

doi:10.1134/s0026261718050120

Cited by 10 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Further increase of gamma irradiation doses results in a noticeable decrease of viability of spores. This result disagree with Kostyleva et al 2018 16 who found that, the most aggressive irradiation dose that gave the highest levels target of the enzyme was 2.5 kGy.…”

Section: Discussioncontrasting

confidence: 76%

Biochemical and Biotechnological Studies on Xylanase and β‐xylosidase Enzymes Produced by Trichoderma viride Under Solid State Fermentation

Farrag¹,

EL-Haw²,

Al-Bokhomy³

2021

J Pure Appl Microbiol

View full text Add to dashboard Cite

The current study concerns with enhancement of xylanase and β-xylosidase activities from lignocellulosic materials by soil derived fungi Trichoderma viride under solid state fermentation (SSF). Xylanase and β-xylosidase activities were found to affected by variety of factors. In this study, some agricultural wastes were selected and used as sources for carbon. Sugar beet pulp (SBP) was at the forefront of these types. Also, various sources for nitrogen were selected to determine the best one. Yeast extract was the best organic source. Maximum xylanase activity took place by using inoculum size 1.8 × 105 spores / ml at 30°C when the pH was 5.5 for eight days of incubation with the addition of 0.1 % of Tween 40. Moreover, spores of Trichoderma viride were irradiated with gamma–rays. The maximum activity was observed upon using 0.7 kilo-gray (kGy). Furthermore, mixed cultures of Trichoderma viride and Penicillium janthenellum (wt / wt) were enhanced xylanase degrading capability. From another standpoint, ammonium sulphate and gel filtration chromatography were the best methods for xylanase purification. Characterizations of the purified enzyme were also selected and studied. It was found that, β-xylosidase enzyme exhibited its maximum activity and stability when the pH was 6 at 40°C by the addition of CaCl2 metal ion. On the other hand, total protein contents and volatile constituents of Trichoderma viride and Penicillium janthenellum were separated and investigated using high performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC/MS) techniques. It was found that, total protein contents for Trichoderma viride and Penicillium janthenellum were represented by fifteen and sixteen amino acids respectively. Also,29 compounds of the total volatile compounds for both Trichoderma viride and Penicillium janthenellum were identified. On the practical and applied field, crude enzyme was a good analyzer for agricultural residues as well showed antifungal and antibacterial effects.

show abstract

Section: Discussioncontrasting

confidence: 76%

Biochemical and Biotechnological Studies on Xylanase and β‐xylosidase Enzymes Produced by Trichoderma viride Under Solid State Fermentation

Farrag¹,

EL-Haw²,

Al-Bokhomy³

2021

J Pure Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Malt çiminin, antibiyotik, aseton, butanol, l-lisin, laktik asit, sitrik asit, amilaz, pektinaz üretimi ve küf, maya ve probiyotik bazı bakterilerin gelişimi için kültür ortamında besin bileşeni olarak kullanıldığı bildirilmiştir [23]. Malt çimi, alkalin 5′fosfodiesteraz [24] ve ksilanaz [25], [26] enzimlerinin biyoteknolojik olarak üretiminde, demir içermeyen ağır ve kıymetli metallerin adsorbsiyonu için biyosorbent olarak [27] kullanılmıştır. Ayrıca, malt çimi ve türevlerinden elde edilen ekstrakt ve maddeler, çeşitli yiyecek ve içeceklerin üretimlerinde de kullanılmıştır [28].…”

Section: Malt çImi/malt çIli (Malt Sprout)unclassified

Valorization of brewery waste

Atalay¹,

Perendeci²,

Göksungur³

2020

Pamukkale J Eng Sci

View full text Add to dashboard Cite

Atık yönetimi, biracılık sektörü de dahil olmak üzere tüm gıda endüstrisi için çevre bileşenlerinin korunması, kirliliğin önlenmesi ve ekonomik üretim için kritik bir öneme sahiptir. Çevre kirliliği, dünya nüfusundaki artış ve buna bağlı olarak daha verimli üretim yöntemlerinin geliştirilmek zorunda olması gibi nedenlerle atık ve yan ürünlerin geri kazanılması ve bu atıklardan katma değeri yüksek ürünlerin üretilmesi zorunlu hale gelmektedir. Bira üreticileri, atıkların yönetimi için ülkeler tarafından yürürlüğe sokulan çevre ile ilgili yasal düzenlemelere uymak zorunda olup, bu konuda ciddi yatırımlar yapmaktadırlar. Bira sanayi için olumsuz bir maliyet unsuru olan organik yapıdaki bira sanayi atıkları, biyoteknolojik süreçler için ucuz maliyeti ve zengin kimyasal kompozisyonu ile gelecek vaat eden bir ham madde kaynağını oluşturmaktadır. Bu çalışmada, bira üretimi sonucunda ortaya çıkan çeşitli atık ve yan ürünler tanımlanmış ve bu atıklar ile yan ürünlerin biyoteknolojik yöntemlerle değerlendirilme olanakları incelenmiştir.Waste management is a critical and important issue for the food industry related to the issues of environmental protection, pollution prevention and process economy. Utilization of waste and by-products for the production of high value-added products becomes compulsory due to economic and environmental issues. Since brewery industry generates large amounts of organic wastes, breweries make investments for the management of wastes according to the environmental regulations forced by the rules of the countries. Although brewery waste is a negative cost factor for brewery industry, its rich content of complex carbohydrates, nitrogen and minerals opens avenues to the production of biotechnological products such as biofuel, enzymes, organic acids and bioactive compounds. In this review, different types of brewery waste and by products are defined and alternative valorization methods and utilization strategies in biotechnological processes are explained.

show abstract

“…4 After several stages of gamma irradiation in a GUT-200 chamber with cobalt-60 as radiation source using severe irradiation regimes of 2000 to 2500 Gray and low survival rates of 0.00014% to 0.01%, a T. reesei-Co-44 mutant was obtained with a significant increase in EG and Xyl activity. 11 It was interesting to determine the carbohydrases composition in EP obtained from the initial and mutant T. reesei strains. So, the strains were cultured in 3-L laboratory fermenters with a working volume of 1.35 L in a fed-batch mode on a medium of the following composition (%): glucose -5.0, microcrystalline cellulose (MCC) -1, corn extract -4.0, KH 2 PO 4 -0.2, (NH 4 ) 2 SO 4 -0.6, CaCl 2 -0.06, antifoam Propinol -0.1, and tap water, pH 4.5.…”

mentioning

confidence: 99%

“…After several stages of gamma irradiation in a GUT-200 chamber with cobalt-60 as radiation source using severe irradiation regimes of 2000 to 2500 Gray and low survival rates of 0.00014% to 0.01%, a T. reesei -Co-44 mutant was obtained with a significant increase in EG and Xyl activity. 11…”

mentioning

confidence: 99%

The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis

et al. 2019

View full text Add to dashboard Cite

The filamentous fungus Trichoderma reesei is traditionally used as the main industrial producer of cellulases and hemicellulases. Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production. In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role. Earlier, we carried out gamma mutagenesis of an industrial strain T. reesei BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex. Endoglucanase activity of the strain was increased 5-fold, while xylanase activity increased more than 8-fold. It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant T. reesei strains. So, the strains were cultured in laboratory fermenters; concentrated preparations were obtained using freeze dryer. It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain. Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant. The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain. The obtained data show the ability of gamma irradiation to affect the component composition of T. reesei carbohydrase complex.

show abstract

Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis

Cited by 10 publications

References 21 publications

Biochemical and Biotechnological Studies on Xylanase and β‐xylosidase Enzymes Produced by Trichoderma viride Under Solid State Fermentation

Biochemical and Biotechnological Studies on Xylanase and β‐xylosidase Enzymes Produced by Trichoderma viride Under Solid State Fermentation

Valorization of brewery waste

The Change in the Composition of Trichoderma reesei Carbohydrases Complex as a Result of Gamma Mutagenesis

Contact Info

Product

Resources

About