Enhanced wound healing by topical administration of mesenchymal stem cells transfected with stromal cell-derived factor-1

Abstract

The objective of this study was to investigate the ability of mesenchymal stem cells (MSC) genetically engineered with stromal cell-derived factor-1 (SDF-1) to heal skin wounds. When transfected with SDF-1 plasmid DNA, MSC which were isolated from the bone marrow of rats, secreted SDF-1 for 7 days. In vitro cell migration assay revealed that the SDF-1-engineered MSC (SDF-MSC) enhanced the migration of MSC and dermal fibroblasts to a significantly greater extent than MSC. The SDF-MSC secreted vascular endothelial growth factor, hepatocyte growth factor, and interleukin 6 at a significantly high level. A skin defect model of rats was prepared and MSC and SDF-MSC were applied to the wound to evaluate wound healing in terms of wound size and histological examinations. The wound size decreased significantly faster with SDF-MSC treatment than with MSC and PBS treatments. The length of the neoepithelium and the number of blood vessels newly formed were significantly larger. A cell-tracing experiment with fluorescently labeled cells demonstrated that the percent survival of SDF-MSC in the tissue treated was significantly high compared with that of MSC. It was concluded that SDF-1 genetic engineering is a promising way to promote the wound healing activity of MSC for a skin defect.

Specific disruption of Lnk in murine endothelial progenitor cells promotes dermal wound healing via enhanced vasculogenesis, activation of myofibroblasts, and suppression of inflammatory cell recruitment

“…Various risk factors, however, such as diabetes, hypoxia, ischemia, and infection, lead to dysfunction of various types of cells and to production of soluble mediators, resulting in wound underhealing or overhealing. Recently, several studies showed that local or systemic administration of stem or progenitor cells, such as mesenchymal stem cells (MSCs) and EPCs, enhances wound repair and angiogenesis [10, 21]. In particular, these studies revealed that the use of genetically engineered cell populations could increase the therapeutic efficacy, because the harsh pathological environment, including hypoxia, drastically affects the survival rate of the unmodified transplanted cells.…”

Wound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model

“…The cell pellets were redispersed in M199 (GIBCO) supplemented with 10% FBS and 1% endothelial cell growth supplement/ heparin kit (ECGS/H, Promocell). Primary dermal fibroblasts were isolated and subcultured using a method described previously [46]. Briefly, the skin specimens were cut into small pieces with surgical scissors, placed in a 10 cm tissue culture dish and cultured in DMEM supplemented with 10 vol% FCS and 1 wt.% P/S at 37 C in a humidified atmosphere composed of 5% CO 2 .…”

A Cooperative Copper Metal–Organic Framework‐Hydrogel System Improves Wound Healing in Diabetes

“…[1] In particular, chronic diabetic foot ulcers (DFUs) are responsible for more than 73,000 nontraumatic lower limb amputations and impose a substantial cost burden on public and private payers, with costs ranging from $9–$13 billion in addition to the costs associated with diabetes itself. [2] Despite the use of autografts, tissue engineered products, and wound dressings of various types, [3] successful treatment of chronic DFUs remains elusive and currently there is no widely used effective therapy. [4] Therefore, new cost-effective, safe, and efficacious strategies are warranted to improve the care for hard-to-heal DFUs.…”