Enhanced vascular regeneration with chemically/physically treated bovine/human pericardium in rodents

Steenberghe, Mathieu van; Schubert, Thomas; Xhema, Daela; Bouzin, Caroline; Guiot, Yves; Duisit, Jérôme; Abdelhamid, Karim; Gianello, Pierre

doi:10.1016/j.jss.2017.09.043

Cited by 10 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, the NaOH-based process showed previously good clinical results regarding biocompatibility and cytotoxicity for other allogeneic biological tissues in different implantation sites with good host cell incorporation and remodeling [ 18 , 19 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Steenberghe

Schubert

Gerelli

et al. 2018

J Cardiothorac Surg

Self Cite

View full text Add to dashboard Cite

BackgroundGlutaraldehyde fixed xenogeneic heart valve prosthesis are hindered by calcification and lack of growth potential. The aim of tissue decellularization is to remove tissue antigenicity, avoiding the use of glutaraldehyde and improve valve integration with low inflammation and host cell recolonization. In this preliminary study, we investigated the efficacy of a NaOH-based process for decellularization and biocompatibility improvement of porcine pulmonary heart valves in comparison to a detergent-based process (SDS-SDC0, 5%).MethodsNative cryopreserved porcine pulmonary heart valves were treated with detergent and NaOH-based processes.Decellularization was assessed by Hematoxylin and eosin/DAPI/alpha-gal/SLA-I staining and DNA quantification of native and processed leaflets, walls and muscles.Elongation stress test investigated mechanical integrity of leaflets and walls (n = 3 tests/valve component) of valves in the native and treated groups (n = 4/group).Biochemical integrity (collagen/elastin/glycosaminoglycans content) of leaflet-wall and muscle of the valves (n = 4/group) was assessed and compared between groups with trichrome staining (Sirius Red/Miller/Alcian blue).Secondly, a preliminary in vivo study assessed biocompatibility (CD3 and CD68 immunostaining) and remodeling (Hematoxylin and eosin/CD31 and ASMA immunofluorescent staining) of NaOH processed valves implanted in orthotopic position in young Landrace pigs, at 1 (n = 1) and 3 months (n = 2).ResultsDecellularization was better achieved with the NaOH-based process (92% vs 69% DNA reduction in the wall). Both treatments did not significantly alter mechanical properties. The detergent-based process induced a significant loss of glycosaminoglycans (p < 0,05).In vivo, explanted valves exhibited normal morphology without any sign of graft dilatation, degeneration or rejection. Low inflammation was noticed at one and three months follow-up (1,8 +/− 3,03 and 0,9836 +/− 1,3605 CD3 cells/0,12 mm2 in the leaflets). In one animal, at three months we documented minimal calcification in the area of sinus leaflet and in one, microthrombi formation on the leaflet surface at 1 month. The endoluminal side of the valves showed partial reendothelialization.ConclusionsNaOH-based process offers better porcine pulmonary valve decellularization than the detergent process. In vivo, the NaOH processed valves showed low inflammatory response at 3 months and partial recellularization. Regarding additional property of securing, this treatment should be considered for the new generation of heart valves prosthesis.Graphical abstractGraphical abstract of the study

show abstract

Section: Discussionmentioning

confidence: 99%

“…We previously demonstrated enhanced biocompatibility and vascular remodeling of allogeneic and xenogeneic pericardium with a treatment based on NaOH decellularization in comparison to the glutaraldehyde fixation and detergent process [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Steenberghe

Schubert

Gerelli

et al. 2018

J Cardiothorac Surg

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this direction, many authors are working towards the decellularization of human pericardium to obtain ECM-derived scaffolds for cardiovascular TE. Human pericardial samples were obtained as discharge material from cadavers [149,150,151,152,153,154,155] or from consenting patients scheduled for cardiothoracic surgery [156,157,158,159]. Currently, various decellularization methods have been reported (Table 4), most of which are based on the use of detergent–enzymatic treatment [149,150,151,152,155,158,159].…”

Section: Heartmentioning

confidence: 99%

“…Currently, various decellularization methods have been reported (Table 4), most of which are based on the use of detergent–enzymatic treatment [149,150,151,152,155,158,159]. Furthermore, recent studies successfully tested the SDS-based decellularization method [156] and a novel protocol based on the use of less traditional chemical agents (i.e., acetone and ethanol) [153,154,155]. All these processes showed a good decellularization rate and a complete removal of cell surface antigens, without affecting ECM structure.…”

Section: Heartmentioning

confidence: 99%

“…Chemically decellularized pericardial patches were in vivo tested into animal models of arterial wall regeneration, being implanted as a vascular patch on the abdominal aorta of rats [153,154] or the carotid/aorta of Vietnamese pigs [155]. These intravascular studies highlighted enhanced graft integration, low immunogenicity, and recellularization of scaffolds.…”

Section: Heartmentioning

confidence: 99%

See 1 more Smart Citation

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Porzionato

Stocco

Barbon

et al. 2018

IJMS

253

191

View full text Add to dashboard Cite

Tissue engineering and regenerative medicine involve many different artificial and biologic materials, frequently integrated in composite scaffolds, which can be repopulated with various cell types. One of the most promising scaffolds is decellularized allogeneic extracellular matrix (ECM) then recellularized by autologous or stem cells, in order to develop fully personalized clinical approaches. Decellularization protocols have to efficiently remove immunogenic cellular materials, maintaining the nonimmunogenic ECM, which is endowed with specific inductive/differentiating actions due to its architecture and bioactive factors. In the present paper, we review the available literature about the development of grafts from decellularized human tissues/organs. Human tissues may be obtained not only from surgery but also from cadavers, suggesting possible development of Human Tissue BioBanks from body donation programs. Many human tissues/organs have been decellularized for tissue engineering purposes, such as cartilage, bone, skeletal muscle, tendons, adipose tissue, heart, vessels, lung, dental pulp, intestine, liver, pancreas, kidney, gonads, uterus, childbirth products, cornea, and peripheral nerves. In vitro recellularizations have been reported with various cell types and procedures (seeding, injection, and perfusion). Conversely, studies about in vivo behaviour are poorly represented. Actually, the future challenge will be the development of human grafts to be implanted fully restored in all their structural/functional aspects.

show abstract

Synergy in a detergent combination results in superior decellularized bovine pericardial extracellular matrix scaffolds

2020

View full text Add to dashboard Cite

Decellularization involves removal of cellular material from tissue which results in a scaffold material consisting of only the extra cellular matrix (ECM). The effect of each individual decellularizing detergent on the final ECM scaffold and how that may differ from the combined use of these detergents is currently a gap in decellularization methodologies. This study evaluates the hypothesis that a synergistic effect exists when commonly used decellularization detergents are combined. This was evaluated with regard to decellularization efficiency, tissue strength, and collagen structure. Bovine pericardium was decellularized using a combination of 0.5% sodium dodecyl sulfate (SDS), 1% sodium deoxycholate (SDC) and 1% TritonX‐100, and compared to the use of each detergent individually. The combined detergent decellularization protocol showed effective decellularization (p = .004), with minimal effects on tissue strength (p = .21) and structure (p = .21). Use of detergents individually, resulted in detrimental effects on tissue structure and integrity or ineffective decellularization. This study shows a synergistic relationship between SDS, SDC and TritonX‐100 when combined at specific concentrations. The use of detergents in combination instead of individually appears to be superior, as it results in less ECM damage and improved decellularization effectivity.

show abstract

Enhanced vascular regeneration with chemically/physically treated bovine/human pericardium in rodents

Cited by 10 publications

References 25 publications

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Synergy in a detergent combination results in superior decellularized bovine pericardial extracellular matrix scaffolds

Contact Info

Product

Resources

About