Enhanced urinary stability of peptide hormones and growth factors by dried urine microsampling

Protti, Michele; Sberna, Paolo; Sberna, Angelo Eliseo; Ferrante, Renzo; Mandrioli, Roberto; Mercolini, Laura

doi:10.1016/j.jpba.2021.114234

Cited by 15 publications

(12 citation statements)

References 24 publications

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“…Among them, and especially in recent years, methods using microsampling techniques are becoming commonplace. These techniques can provide several advantages over traditional venepuncture sampling, including reduced invasiveness, increased analyte stability, and minimal storage and shipping requirements [109][110][111][112]. For example, LC-MS/MS was used to analyze 14 drugs using volumetric absorptive microsampling (VAMS) [113], a technique based on a plastic device with a porous polymeric tip that is able to absorb fixed biological matrix volumes [114][115][116].…”

Section: Multi-drug Methodsmentioning

confidence: 99%

Ion-Channel Antiepileptic Drugs: An Analytical Perspective on the Therapeutic Drug Monitoring (TDM) of Ezogabine, Lacosamide, and Zonisamide

et al. 2021

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The term seizures includes a wide array of different disorders with variable etiology, which currently represent one of the most important classes of neurological illnesses. As a consequence, many different antiepileptic drugs (AEDs) are currently available, exploiting different activity mechanisms and providing different levels of performance in terms of selectivity, safety, and efficacy. AEDs are currently among the psychoactive drugs most frequently involved in therapeutic drug monitoring (TDM) practices. Thus, the plasma levels of AEDs and their metabolites are monitored and correlated to administered doses, therapeutic efficacy, side effects, and toxic effects. As for any analytical endeavour, the quality of plasma concentration data is only as good as the analytical method allows. In this review, the main techniques and methods are described, suitable for the TDM of three AEDs belonging to the class of ion channel agents: ezogabine (or retigabine), lacosamide, and zonisamide. In addition to this analytical overview, data are provided, pertaining to two of the most important use cases for the TDM of antiepileptics: drug–drug interactions and neuroprotection activity studies. This review contains 146 references.

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Section: Multi-drug Methodsmentioning

confidence: 99%

Ion-Channel Antiepileptic Drugs: An Analytical Perspective on the Therapeutic Drug Monitoring (TDM) of Ezogabine, Lacosamide, and Zonisamide

et al. 2021

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“…Dried blood spots (DBS) are gaining importance in anti-doping because of ease in sample collection, transportation, and storage [37][38][39][40][41][42]. In addition to DBS, dried urine spots (DUS) and volumetric absorptive microsampling (VAMS) are used in the analysis of AAS, glucocorticoids, and less stable peptide hormones [43][44][45].…”

Section: Ms and Metabolomic Approach Used In Anti-dopingmentioning

confidence: 99%

“…DUS and VAMS are effective strategies for collecting urine samples, especially for peptide hormones, that are highly susceptible to degradation in fluid conditions. Further, the study highlighted that peptides have remarkably improved stability even at room temperature in the dried state [43].…”

Section: Application Of Ms Techniques To Different Classes Of Prohibi...mentioning

confidence: 99%

Mass spectrometry: A key tool in anti‐doping

Dhurjad

Jaiswal

Gupta

et al. 2022

Separation Science Plus

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Performance enhancement drugs build muscle mass and increase stamina in athletes, making them a potential candidate for abuse in sports competitions. World Anti‐Doping Agency is an international organization responsible for developing standard guidelines, listing agents prohibited for usage in all athletic events, and taking stringent actions for any discrepancies. Sophisticated analytical instruments and statistical tools are regularly used for the chemical fingerprinting of doping agents in different biological matrices. There is an ever‐increasing demand for developing novel and sensitive mass spectrometry methods for detecting new substances of abuse. This review focuses on applying hyphenated chromatographic techniques, emphasizing advanced mass spectrometric methods to analyze different classes of prohibited substances listed by the World Anti‐Doping Agency.

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“…Also here, MS‐based approaches have been substantially optimized over the past decade as comprehensively summarized by Judak et al, 82 and metabolites (and/or catabolites) were shown in selected cases to represent the better target analytes in both blood and urine. Accepting the limitation of sample volume, one option to reduce degradation processes concerning peptidic drugs in urine was presented by Protti et al, 83 who demonstrated a considerable improvement in analyte stability when preparing dried urine spots in analogy to DBS. Using 12 model compounds encompassing molecular masses of 800–3,000 Da (including GH releasing peptides, GHRPs, a GH fragment, adrenocorticotrophic hormone, and a human chorionic gonadotrophin fragment), the superior stability of the intact drugs as dried matrix spots as compared to conventional urine (also under frozen conditions) was shown, corroborating the fact that long‐term storage of urine for retesting programs might require the consideration of metabolites, catabolites, and/or degradation products of peptidic drugs rather than the intact substances alone.…”

Section: Peptide Hormones Growth Factors Related Substances and Mimeticsmentioning

confidence: 99%

Annual banned‐substance review: Analytical approaches in human sports drug testing 2020/2021

Thevis

Kuuranne

Geyer

2021

Drug Testing and Analysis

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Most core areas of anti‐doping research exploit and rely on analytical chemistry, applied to studies aiming at further improving the test methods' analytical sensitivity, the assays' comprehensiveness, the interpretation of metabolic profiles and patterns, but also at facilitating the differentiation of natural/endogenous substances from structurally identical but synthetically derived compounds and comprehending the athlete's exposome. Further, a continuously growing number of advantages of complementary matrices such as dried blood spots have been identified and transferred from research to sports drug testing routine applications, with an overall gain of valuable additions to the anti‐doping field. In this edition of the annual banned‐substance review, literature on recent developments in anti‐doping published between October 2020 and September 2021 is summarized and discussed, particularly focusing on human doping controls and potential applications of new testing strategies to substances and methods of doping specified in the World Anti‐Doping Agency's 2021 Prohibited List.

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Enhanced urinary stability of peptide hormones and growth factors by dried urine microsampling

Cited by 15 publications

References 24 publications

Ion-Channel Antiepileptic Drugs: An Analytical Perspective on the Therapeutic Drug Monitoring (TDM) of Ezogabine, Lacosamide, and Zonisamide

Ion-Channel Antiepileptic Drugs: An Analytical Perspective on the Therapeutic Drug Monitoring (TDM) of Ezogabine, Lacosamide, and Zonisamide

Mass spectrometry: A key tool in anti‐doping

Annual banned‐substance review: Analytical approaches in human sports drug testing 2020/2021

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