Enhanced topical delivery and ex vivo anti-inflammatory activity from a betamethasone dipropionate formulation containing fish oil

Zulfakar, Mohd Hanif; Abdelouahab, Nassima; Heard, Charles Martin

doi:10.1007/s00011-009-0065-z

Cited by 32 publications

(27 citation statements)

References 31 publications

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“…PTGES, PTGES2) and EP4 receptor (PTGER4 ) was significantly downregulated by betamethasone treatment, while EP2 receptor ( PTGER2 ) expression was not changed (Fig 6A). This observation is consistent with findings that betamethasone treatment reduces PGE 2 production46,47. Interestingly, expression of PTGES, PTGES2 and PTGER4 , but not PTGER2 , positively correlated with IL22 expression in atopic biopsies at baseline, while expression of HPGD , which mediates PGE 2 degradation, negatively correlated with IL22 expression (Fig 6B).…”

Section: Resultssupporting

confidence: 90%

Prostaglandin E2 stimulates adaptive IL-22 production and promotes allergic contact dermatitis

Robb

McSorley

Lee

et al. 2018

Journal of Allergy and Clinical Immunology

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Background Atopic dermatitis (AD) and allergic contact dermatitis (ACD) are both forms of eczema and are common inflammatory skin diseases with a central role of Th22-derived IL-22 in their pathogenesis. Although prostaglandin E2 (PGE2) is known to promote inflammation, little is known about its role in processes related to AD and ACD development, including IL-22 up-regulation. Objectives To investigate whether PGE2 has a role in IL-22 induction and development of ACD, which has increased prevalence in patients with AD. Methods T-cell cultures and in vivo sensitization of mice with haptens were used to assess the role of PGE2 in production of IL-22. The involvement of PGE2 receptors and their downstream signals were also examined. The effects of PGE2 were evaluated using the oxazolone (OXA)-induced ACD mouse model. The relationship of PGE2 and IL-22 signaling pathways in skin inflammation were also investigated using genomic profiling in human lesional AD skin. Results PGE2 induces IL-22 from T cells through its receptors EP2 and EP4 and involves cyclic adenosine monophosphate (cAMP) signaling. Selective deletion of EP4 in T-cells prevents hapten-induced IL-22 production in vivo, and inhibition of PGE2 synthesis limits atopic-like skin inflammation in the OXA-induced ACD model. Moreover, both PGE2 and IL-22 pathway genes were coordinately up-regulated in human AD lesional skin, but were below significant detection levels after corticosteroid or ultraviolet band B (UVB) treatments. Conclusions Our results define a crucial role for PGE2 in promoting ACD by facilitating IL-22 production from T-cells.

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Section: Resultssupporting

confidence: 90%

Prostaglandin E2 stimulates adaptive IL-22 production and promotes allergic contact dermatitis

Robb

McSorley

Lee

et al. 2018

Journal of Allergy and Clinical Immunology

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“…Its various dosage forms such as ointment, cream, lotion, and foam are in use for the treatment of mild to moderate psoriasis. [23] However, the clinical use of BD has some practical disadvantages such as poor permeability through skin which reduces its therapeutic effectiveness at the target site. The main limitation lies in the barrier function of the skin, which is considered one of the most impermeable epithelia of the human body to exogenous substances.…”

Section: Introductionmentioning

confidence: 99%

Nanocarrier-based hydrogel of betamethasone dipropionate and salicylic acid for treatment of psoriasis

Baboota

Alam

Sharma

et al. 2011

Int J Pharma Investig

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Introduction:Betamethasone dipropionate (BD) has anti-inflammatory, immunomodulatory, and antiproliferative activity. The aim of the current work was to test the hypothesis that the addition of corticosteroid such as BD and a keratolytic agent such as salicylic acid in nanocarrier based microemulsions formulation would result in enhancement and sustaining of corticosteroid delivery rate leading to better anti-psoriatic activity. Clinical use of BD is restricted to some extent due to its poor permeability across the skin. So to increase its permeation across the skin, microemulsion-based gel formulations were prepared and characterised.Materials and Methods:Microemulsions were prepared by aqueous phase titration method, using oleic acid:sefsol (1.5:1), Tween 20, isopropyl alcohol, and distilled water as the oil phase, surfactant, cosurfactant and aqueous phase, respectively. Selected formulations were subjected to physical stability studies and consequently in vitro skin permeation studies. Surface studies of optimized formulation were done by transmission electron microscopy. In vivo anti-inflammatory activity was done by carageenan-induced raw paw edema method.Results:The droplet size of microemulsions ranged from 60 to 190 nm. The optimized formulation exhibited viscosity 28.55 ± 2.03 mP, refractive index 1.409, pH 6.4, and conductivity 10-4 scm-1. The optimized microemulsion was converted into hydrogel using carbopol 934, and salicylic acid was incorporated into it. Drug deposition in skin was found to be 29.73 μg/mg. Assessment of skin permeation was done by histopathology studies which indicated changes in the structure of epidermal membrane of skin. In vivo anti-inflammatory activity indicated 72.11% and 43.96% inhibition of inflammation in case of developed microemulsion gel and marketed gel, respectively.Conclusions:The developed microemulsion gel containing BD and salicylic acid provided sustained and good anti-inflammatory activity for the treatment of psoriasis.

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“…MTX is known to be anti-inflammatory, and one way of observing this effect is by determining modulation in levels of the inflammation marker, PGE 2 [21]. Meanwhile, this bioassay can also indirectly indicate the extent of MTX delivery into the skin, as any reduction in PGE 2 must be a consequence of MTX activity on the viable keratinocytes.…”

Section: Determination Of Pge 2 In Porcine Skin Ex Vivomentioning

confidence: 98%

Enhanced topical delivery and anti-inflammatory activity of methotrexate from an activated nanogel

Singka

Samah

Zulfakar

et al. 2010

European Journal of Pharmaceutics and Biopharmaceutics

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Enhanced topical delivery and ex vivo anti-inflammatory activity from a betamethasone dipropionate formulation containing fish oil

Abstract: Fish oil enhanced the delivery of BD and SA across skin. Addition of fish oil also enhanced the anti-inflammatory activity of BD, attributed to increased amounts of BD present in the skin and/or the intrinsic anti-inflammatory activity of fish oil.

Cited by 32 publications

References 31 publications

Prostaglandin E2 stimulates adaptive IL-22 production and promotes allergic contact dermatitis

Prostaglandin E2 stimulates adaptive IL-22 production and promotes allergic contact dermatitis

Nanocarrier-based hydrogel of betamethasone dipropionate and salicylic acid for treatment of psoriasis

Enhanced topical delivery and anti-inflammatory activity of methotrexate from an activated nanogel

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