Enhanced Tolerance to Chilling Stress in <i>OsMYB3R-2</i> Transgenic Rice Is Mediated by Alteration in Cell Cycle and Ectopic Expression of Stress Genes

Ma, Qibin; Dai, Xianjun; Xu, Yunyuan; Guo, Jing; Liu, Yaju; Chen, Na; Xiao, Jun; Zhang, Dajian; Xu, Zhi-Hong; Zhang, Xiansheng; Chong, Kang

doi:10.1104/pp.108.133454

Cited by 300 publications

(216 citation statements)

References 70 publications

(115 reference statements)

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“…Overexpression of DRE-B1A/CBF3 induces DREB1, DREB2, and RD29A genes and confers increased resistance to drought, high-salt, and freezing stresses [41]. Although OE-OsMYB3R-2 exhibited enhanced cold tolerance in rice, it also resulted in growth retardation under normal conditions [42]. Similarly, OE-DREB1A in Arabidopsis caused severe growth retardation [41], and overexpression of CBF1 in tomato resulted in a dwarf phenotype [43].…”

Section: Discussionmentioning

confidence: 99%

The unique mode of action of a divergent member of the ABA-receptor protein family in ABA and stress signaling

et al. 2013

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Proteins in the PYR/PYL/RCAR family (PYLs) are known as receptors for the phytohormone ABA. Upon ABA binding, PYL adopts a conformation that allows it to interact with and inhibit clade A protein phosphatase 2Cs (PP2Cs), which are known as the co-receptors for ABA. Inhibition of the PP2Cs then leads to the activation of the SnRK2 family protein kinases that phosphorylate and activate downstream effectors in ABA response pathways. The PYL family has 14 members in Arabidopsis, 13 of which have been demonstrated to function as ABA receptors. The function of PYL13, a divergent member of the family, has been enigmatic. We report here that PYL13 differs from the other PYLs in three key residues that affect ABA perception, and mutations in these three residues can convert PYL13 into a partially functional ABA receptor. Transgenic plants overexpressing PYL13 show increased ABA sensitivity in seed germination and postgermination seedling establishment as well as decreased stomatal conductance, increased water-use efficiency, accelerated stress-responsive gene expression, and enhanced drought resistance. pyl13 mutant plants are less sensitive to ABA inhibition of postgermination seedling establishment. PYL13 interacts with and inhibits some members of clade A PP2Cs (PP2CA in particular) in an ABA-independent manner. PYL13 also interacts with the other PYLs and antagonizes their function as ABA receptors. Our results show that PYL13 is not an ABA receptor but can modulate the ABA pathway by interacting with and inhibiting both the PYL receptors and the PP2C co-receptors.

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Section: Discussionmentioning

confidence: 99%

The unique mode of action of a divergent member of the ABA-receptor protein family in ABA and stress signaling

et al. 2013

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“…For instance, overexpression of OsMYB4 significantly enhanced tolerance to chilling and freezing stress in transgenic Arabidopsis (Vannini et al 2004;Pasquali et al 2008). Ma et al (2009) reported that OsMYB3R-2 participated in cold signalling pathway by targeting the cell cycle and a putative DREB/CBF. Moreover, a recent study revealed that OsMYBS3 was essential for conferring tolerance to cold stress in rice plants (Su et al 2010) while R2R3MYB gene, OsMYB2, was involved in salt, cold, and dehydration tolerance in rice ).…”

Section: Abiotic Stressmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

817

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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“…Furthermore, many of the genes in the ATTED coexpression clusters of these genes are coordinately upregulated (see Supplemental Figure 14 and Supplemental Table 3 online), suggesting that plants either experience stress by RBR downregulation or that a general stress signaling pathway is attenuated by RBR and becomes deregulated by small changes in RBR activity. Links between cell cycle responses and different stresses have been made for plants (Ma et al, 2009) and animal cells (Burhans and Heintz, 2009;Mavrogonatou and Kletsas, 2009), but the mechanisms underlying the crosstalk between cell cycle and stresses remain unknown.…”

Section: Gene Expression Regulation By Rbrmentioning

confidence: 99%

ArabidopsisRETINOBLASTOMA-RELATED Is Required for Stem Cell Maintenance, Cell Differentiation, and Lateral Organ Production

et al. 2010

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Several genes involved in the regulation of postembryonic organ initiation and growth have been identified. However, it remains largely unclear how developmental cues connect to the cell cycle. RETINOBLASTOMA RELATED (RBR) is a plant homolog of the tumor suppressor Retinoblastoma (pRb), which is a key regulator of the cell cycle. Using inducible RNA interference (RNAi) against Arabidopsis thaliana RBR (RBRi), we reduced RBR expression levels at different stages of plant development. Conditional reduction or loss of RBR function disrupted cell division patterns, promoted context-dependent cell proliferation, and negatively influenced establishment of cell differentiation. Several lineages of toti-and pluripotent cells, including shoot apical meristem stem cells, meristemoid mother cells, and procambial cells, failed to produce appropriately differentiated cells. Meristem activity was altered, leading to a disruption of the CLAVATA-WUSCHEL feedback loop and inhibition of lateral organ formation. Release of RBR from RNAi downregulation restored meristem activity. Gene profiling analyses soon after RBRi induction revealed that a change in RBR homeostasis is perceived as a stress, even before genes regulated by RBR-E2F become deregulated. The results establish RBR as a key cell cycle regulator required for coordination of cell division, differentiation, and cell homeostasis. INTRODUCTIONRetinoblastoma (RB) was the first tumor suppressor gene identified in animals (Friend et al., 1986) and later was associated with cell cycle regulation, thus establishing pRb as a key regulator of cell proliferation. In animals, three members of the RB family of proteins (pRb, p107, and p130), also referred to as pocket proteins (Mulligan and Jacks, 1998), negatively regulate the G1-to-S phase transition during the cell cycle. It is now widely accepted that mitogenic signals activate several cyclin-dependent kinase (CDK)-cyclin complexes during progression through G1 to phosphorylate pRb, thereby releasing it from interactions with E2F/DP transcription factor complexes to facilitate S-phase entry (Weinberg, 1995). Subsequently, pRb was also found to regulate tissue-specific transcription factors that regulate cell differentiation, such as MyoD, which activates muscle-specific genes (De Falco et al., 2006). pRb also regulates germ cell proliferation, differentiation, and survival (Toppari et al., 2003) and is required for osteoblast, keratinocyte, and macrophage differentiation (Nead et al., 1998;Liu et al., 1999;Thomas et al., 2003). Together with the tumor suppressor p53, pRb regulates adipocyte differentiation and function (Hallenborg et al., 2009). The requirement for RB in cell differentiation therefore extends beyond regulation of cell cycle entry. For example, the loss of RB function in cardioblasts affects cell differentiation by modulating the activity of cardiogenic factors (Papadimou et al., 2005).RB-related genes, termed RETINOBLASTOMA-RELATED (RBR; Ach et al., 1997), are also found in monocots (Grafi et al., 1996;Xie et al...

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Enhanced Tolerance to Chilling Stress in OsMYB3R-2 Transgenic Rice Is Mediated by Alteration in Cell Cycle and Ectopic Expression of Stress Genes

Cited by 300 publications

References 70 publications

The unique mode of action of a divergent member of the ABA-receptor protein family in ABA and stress signaling

The unique mode of action of a divergent member of the ABA-receptor protein family in ABA and stress signaling

MYB transcription factor genes as regulators for plant responses: an overview

ArabidopsisRETINOBLASTOMA-RELATED Is Required for Stem Cell Maintenance, Cell Differentiation, and Lateral Organ Production

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