Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine

Padilla-Martínez, Silvia G.; Martínez-Jothar, Lucía; Sampedro, José G.; Tristan, Ferdinando; Pérez, Elı́as

doi:10.1016/j.ijbiomac.2015.02.005

Cited by 24 publications

(17 citation statements)

References 37 publications

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“…The graph shows a slight shifting of the enzyme activity optimal temperature between the free and immobilized conditions. Free GOx displayed a maximum activity at 50 °C which decreased by 50% at ~60 °C and continued to decline until the activity is absent at 75 °C indicating enzyme denature due to temperature-related destruction of the enzyme structure as consistent to the literature 59,60 . In contrast, immobilized GOx reaches the highest activity at 55 °C which decreased by 50% at ~65 °C indicating an increase in improvement of thermal stability of the immobilized enzyme.…”

Section: Resultssupporting

confidence: 83%

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Morshed

Behary

Bouazizi

et al. 2019

Sci Rep

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Robust immobilization of glucose oxidase (GOx) enzyme was achieved on poly(ethylene terephthalate) nonwoven fabric (PN) after integration of favourable surface functional groups through plasma treatments [atmospheric pressure-AP or cold remote plasma-CRP (N2 + O2)] and/or chemical grafting of hyperbranched dendrimers [poly-(ethylene glycol)-OH or poly-(amidoamine)]. Absorption, stability, catalytic behavior of immobilized enzymes and reusability of resultant fibrous bio-catalysts were comparatively studied. Full characterization of PN before and after respective modifications was carried out by various analytical, instrumental and arithmetic techniques. Results showed that modified polyester having amine terminal functional groups pledged better surface property providing up to 31% enzyme loading, and 81% active immobilized enzymes. The activity of the enzyme was measured in terms of interaction aptitude of GOx in a given time to produce hydrogen peroxide using colorimetric assay. The immobilized GOx retained 50% of its original activity after being reused six (06) times and exhibited improved stability compared with the free enzyme in relation to temperature. The reaction kinetics, loading efficiency, leaching, and reusability analysis of enzyme allowed drawing a parallel to the type of organic moiety integrated during GOx immobilization. In addition, resultant fibrous bio-catalysts showed substantial antibacterial activity against pathogenic bacteria strains (Staphylococcus epidermidis and Escherichia coli) in the presence of oxygen and glucose. These results are of great importance because they provide proof-of-concept for robust immobilization of enzymes on surface-modified fibrous polyester fabric for potential bio-industrial applications.

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Section: Resultssupporting

confidence: 83%

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Morshed

Behary

Bouazizi

et al. 2019

Sci Rep

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“…Also, when speaking about thermo-responsive properties, which are correlated with the principal moment of inertia, LPEIs should be chosen over BPEIs. The structural principal moment is related to temperature and thus the thermal (in)stability of the GOx-PEI complex [29]. The optimal size of PEI must be around C18N10, which is at about the middle of the length of PEI chain, as suggested by the QSAR model developed in this study, in view of getting evidence of a certain “saturation” of GOx by PEI, and vice versa , when the size of the PEI molecule increases.…”

Section: Discussionmentioning

confidence: 95%

Linear and Branched PEIs (Polyethylenimines) and Their Property Space

Lungu

Diudea

Putz

et al. 2016

IJMS

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A chemical property space defines the adaptability of a molecule to changing conditions and its interaction with other molecular systems determining a pharmacological response. Within a congeneric molecular series (compounds with the same derivatization algorithm and thus the same brute formula) the chemical properties vary in a monotonic manner, i.e., congeneric compounds share the same chemical property space. The chemical property space is a key component in molecular design, where some building blocks are functionalized, i.e., derivatized, and eventually self-assembled in more complex systems, such as enzyme-ligand systems, of which (physico-chemical) properties/bioactivity may be predicted by QSPR/QSAR (quantitative structure-property/activity relationship) studies. The system structure is determined by the binding type (temporal/permanent; electrostatic/covalent) and is reflected in its local electronic (and/or magnetic) properties. Such nano-systems play the role of molecular devices, important in nano-medicine. In the present article, the behavior of polyethylenimine (PEI) macromolecules (linear LPEI and branched BPEI, respectively) with respect to the glucose oxidase enzyme GOx is described in terms of their (interacting) energy, geometry and topology, in an attempt to find the best shape and size of PEIs to be useful for a chosen (nanochemistry) purpose.

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“…Generally, stability for pH [51][52][53][54], heat [52][53][54][55][56][57][58], and other properties [59][60][61][62][63][64][65][66][67][68][69][70][71] is improved by the immobilization of the enzymes. However, as mentioned above, improvements in enzyme properties such as heat tolerance and pH stability are effective in enhancing stability.…”

Section: Supporting Materialsmentioning

confidence: 99%

How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

Yabuki

2017

Catalysts

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Abstract:In this review, factors that contribute to enhancing the stability of immobilized enzyme membranes have been indicated, and the solutions to each factor, based on examples, are discussed. The factors are divided into two categories: one is dependent on the improvement of enzyme properties, and the other, on the development of supporting materials. Improvement of an enzyme itself would effectively improve its properties. However, some novel materials or novel preparation methods are required for improving the properties of supporting materials. Examples have been provided principally aimed at improvements in membrane stability.

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Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine

Cited by 24 publications

References 37 publications

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Linear and Branched PEIs (Polyethylenimines) and Their Property Space

How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

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