Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Abstract

Robust immobilization of glucose oxidase (GOx) enzyme was achieved on poly(ethylene terephthalate) nonwoven fabric (PN) after integration of favourable surface functional groups through plasma treatments [atmospheric pressure-AP or cold remote plasma-CRP (N2 + O2)] and/or chemical grafting of hyperbranched dendrimers [poly-(ethylene glycol)-OH or poly-(amidoamine)]. Absorption, stability, catalytic behavior of immobilized enzymes and reusability of resultant fibrous bio-catalysts were comparatively studied. Full characterization of PN before and after respective modifications was carried out by various analytical, instrumental and arithmetic techniques. Results showed that modified polyester having amine terminal functional groups pledged better surface property providing up to 31% enzyme loading, and 81% active immobilized enzymes. The activity of the enzyme was measured in terms of interaction aptitude of GOx in a given time to produce hydrogen peroxide using colorimetric assay. The immobilized GOx retained 50% of its original activity after being reused six (06) times and exhibited improved stability compared with the free enzyme in relation to temperature. The reaction kinetics, loading efficiency, leaching, and reusability analysis of enzyme allowed drawing a parallel to the type of organic moiety integrated during GOx immobilization. In addition, resultant fibrous bio-catalysts showed substantial antibacterial activity against pathogenic bacteria strains (Staphylococcus epidermidis and Escherichia coli) in the presence of oxygen and glucose. These results are of great importance because they provide proof-of-concept for robust immobilization of enzymes on surface-modified fibrous polyester fabric for potential bio-industrial applications.

Tunable Polymeric Scaffolds for Enzyme Immobilization

“…The multiple branches and the high density of functional end-groups of the dendrimers enable the high effectiveness of immobilization through covalent bonding. For example, polyester or poly(amidoamine) (PAMAM) dendrimers can be used as a convenient platform for glucose oxidase or lipase enzymes ( Fan et al, 2017 ; Morshed et al, 2019 ). Dendrimer-enzyme hybrids, mostly PAMAM-based dendrimer particles, are also employed for delivery purposes in biomedicine.…”

Cell mimicry as a bottom‐up strategy for hierarchical engineering ofnature‐inspiredentities

“…In addition to the above‐mentioned ArMs, compounds with macrocyclic cavities, either natural or synthetic, which could form inclusion complex with substrates via noncovalent interactions, such as cyclodextrin (Wang, Qu, et al, 2017; Wang & Bols 2017), cucurbituril (Kubota et al, 2018), crown ether (Ning, Ao, Wang, & Wang, 2018), and so on were widely considered to provide this microenvironment to confine substrates and allow for more effective and selective catalysis. Except for these macromolecular models, self‐assembled nanocompartments, such as molecular cages (Marti‐Centelles, Lawrence, & Lusby, 2018; Nurttila et al, 2019; Roy et al, 2017), micelles (Arifuzzaman & Zhao, 2018; Dou et al, 2017), vesicles (Blackman et al, 2018; Fuhrmann et al, 2018), dendrimers (Li et al, 2018; Morshed et al, 2019), and core–shell particles (Keller, Beloqui, Martinez‐Martinez, Ferrer, & Delaittre, 2017), which could immobilize either natural enzymes or AEs and generate a protective and substrate‐dependent environment for the active molecules and improve the catalytic efficiency and selectivity, were also extensively evaluated. We recently demonstrated the preparation of micelles loaded with a salen‐manganese complex (EUK), which exhibited catalase‐like activity (Figure 1bi,bii; Ade et al, 2019).…”

Modification of fibrous membrane for organic and pathogenic contaminants removal: from design to application

“…Polyester membrane-PET (a exible, porous, recyclable, nontoxic, biocompatible material with resistance to most chemical substances and microorganism) has successfully described as a feasible support material to immobilize various organic and inorganic catalyst and it demonstrated to have increased stability and to allow mobility of materials. 27 Since the polyester surface is hydrophobic and non-interactive to most organicinorganic substances, surface activation of the PET membrane is necessary. Among many surface activation processes [28][29][30] of polyester, plasma treatment is more desirable due to their costeffectiveness and eco-friendliness (no harmful solvent, no chemical waste and less destruction of the specimen).…”