Enhanced thebaine production in<i>Papaver bracteatum</i>cell suspension culture by combination of elicitation and precursor feeding

Zare, Nasser; Farjaminezhad, Reza; Zakaria, Rasool Asghari; Farjaminezhad, Manoochehr

doi:10.1080/14786419.2013.878936

Cited by 21 publications

(11 citation statements)

References 26 publications

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“…Likewise, MeJA + Phe produced an accumulation of phenols higher than the untreated samples (+ 56%) but lower than the MeJA + Tyr treatment. These data are in agreement with those reported for cell suspensions of other species such as Withania somnifera , Papaver bracteatum, Catharanthus roseus , and Vitis vinifera [26,28,29].…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, the hemp cell suspensions were treated with MeJA + Phe or with MeJA + Tyr. Phe was chosen because it represents the substrate of PAL, the first committed enzyme of secondary metabolism, and Tyr was chosen since it is the precursor for thousands of specialized compounds [26,27]. Based on the amount of phenols that accumulated, the best combination proved to be MeJA + Tyr during 4 days of treatment, with an 82% phenol increase over the control cells.…”

Section: Discussionmentioning

confidence: 99%

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Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

et al. 2019

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Cannabis sativa L. is one of the most-studied species for its phytochemistry due to the abundance of secondary metabolites, including cannabinoids, terpenes and phenolic compounds. In the last decade, fiber-type hemp varieties have received interest for the production of many specialized secondary metabolites derived from the phenylpropanoid pathway. The interest in these molecules is due to their antioxidant activity. Since secondary metabolite synthesis occurs at a very low level in plants, the aim of this study was to develop a strategy to increase the production of such compounds and to elucidate the biochemical pathways involved. Therefore, cell suspensions of industrial hemp (C. sativa L. var. Futura) were produced, and an advantageous elicitation strategy (methyl jasmonate, MeJA) in combination with precursor feeding (tyrosine, Tyr) was developed. The activity and expression of phenylalanine ammonia-lyase (PAL) and tyrosine aminotransferase (TAT) increased upon treatment. Through 1H-NMR analyses, some aromatic compounds were identified, including, for the first time, 4-hydroxyphenylpyruvate (4-HPP) in addition to tyrosol. The 4-day MeJA+Tyr elicited samples showed a 51% increase in the in vitro assay (2,2-diphenyl-1-picrylhydrazyl, DPPH) radical scavenging activity relative to the control and a 80% increase in the cellular antioxidant activity estimated on an ex vivo model of human erythrocytes. Our results outline the active metabolic pathways and the antioxidant properties of hemp cell extracts under the effect of specific elicitors.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

et al. 2019

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“…The ultrasound treatment at 300 W for 5 min improved the conversion frequencies of protocorm like bodies (PLBs) to shoot in the Dendrobium officinale (Wei et al 2012). In Zare et al (2014) study, the combination of ultrasound (10 s) and L-tyrosine feeding (2 mM) significantly increased the production of thebaine in comparison to individual utilisation of 2 mM L-tyrosine and ultrasound (10 s). 1d-f) data revealed significant differences (P≤0.01) between different PGRs.…”

mentioning

confidence: 85%

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Firoozi¹,

Zare²,

Sofalian³

et al. 2019

Tarım Bilimleri Dergisi

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In the present study, the effects of ultrasound and plant growth regulators on in vitro callogenesis and secondary metabolites production in saffron calli were investigated. Accordingly, the saffron corms surface sterilized, sonicated and cultured on different concentrations of plant growth regulators (0.5, 1, 2 and 4 mg L-1 2,4-D or NAA in combination with 0.5 and 1 mg L-1 Kin or BAP). The percentage of callus induction, callus yield (fresh weight) and embryogenic callus formation were recorded and secondary metabolites of calli were measured by UV/VIS spectrophotometer three months after culture. The results indicated that sonication of the saffron corm explants significantly increased the in vitro callus induction and growth. So, the highest callus induction (100%) and yield (4.68 g) was achieved with sonicated explants cultured on MS medium supplemented with 2 mg L-1 1-naphthaleneacetic acid (NAA) and 0.5 mg L-1 kinetin (Kin). Somatic embryogenesis was significantly influenced by plant growth regulator regimes and the MS medium supplemented with 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) plus 0.5 mg L-1 6-benzylaminopurine (BAP) and 0.5 mg L-1 NAA plus 0.5 mg L-1 Kin exhibited the highest percentage (75 and 72, respectively) of somatic embryogenesis. Secondary metabolite content of the callus cells was significantly different among the plant growth regulator regimes and the highest production of picrocrocin and safranal were occurred on the medium containing 0.5 mg L-1 2,4-D plus 0.5 mg L-1 BAP and 1 mg L-1 NAA plus 1 mg L-1 BAP.

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“…At the end of this period (42 days), several parameters such as the percentage of callus induction, callus morphology, callus fresh weights (CFW) and callus dry weights (CDW) were measured. Cell suspension culture was started according to protocol as descripted by Zare et al (13). Briefly, friable root callus (80-100 mg) transferred into a 50 mL Erlenmeyer flask containing 15 mL of liquid 3:4 MS medium filled with 3 mg L -1 2,4-D and 0.5 mg L -1 BAP.…”

Section: Callus Induction and Cell Suspension Culture Establishmentmentioning

confidence: 99%

“…In plant cells, one of the defense response is the accumulation or even production of secondary metabolites. These responses can be activated by different endogenous and exogenous molecules called elicitors (13). One of the important elicitors, which naturally exist in higher plants is methyl jasmonate (MJ).…”

Section: Introductionmentioning

confidence: 99%

Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.)

Dastmalchi

Omidi

Azizinezhad

et al. 2019

Cell Mol Biol (Noisy-le-grand)

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The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on thebaine and sanguinarine productions in vitro were investigated. Roots as explant and supplementing 3 mg L -1 2,4-Dichlorophenoxyacetic acid with 0.5 mg L -1 Benzyl amino purine to modified MS medium were selected to achieve the most efficient combination for callus induction and production of callus fresh and dry weights.At 48 h after treatment, the addition of PG and MJ individually and in combination together significantly increased both thebaine and sanguinarine contents than the control. The results of high-performance liquid chromatography (HPLC) detection indicated that the highest production rate has been achieved through a synergic effect of two elicitors after 48 h. Results revealed that adding 200 μM of MJ and 100 mg L -1 PG increased thebaine and sanguinarine contents by 56.36 and 107.71-fold than control cells, respectively.

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Enhanced thebaine production inPapaver bracteatumcell suspension culture by combination of elicitation and precursor feeding

Cited by 21 publications

References 26 publications

Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.)

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