Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways

Rubnawaz, Samina; Kayani, Waqas Khan; Mahmood, Rashid; Mirza, Bushra

doi:10.3906/bot-2002-13

Cited by 5 publications

(9 citation statements)

References 52 publications

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“…Multiple in vivo activities including analgesic, anti-inflammatory, antidepressant, and anticoagulant activity was evaluated using BALB/c mice. In the continuation of our previous study [15], estimation of essential elements of transformed regenerated plants of A. bracteosa by atomic absorption spectrophotometry and HPLC fingerprinting of polyphenol content was performed to support our results.…”

Section: Introductionsupporting

confidence: 70%

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

et al. 2021

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Ajuga bracteosa Wall. ex Benth. is an endangered medicinal herb traditionally used against different ailments. The present study aimed to create new insight into the fundamental mechanisms of genetic transformation and the biological activities of this plant. We transformed the A. bracteosa plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. These transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, elemental analysis, polyphenol content, and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was most abundant in all transgenic lines. Furthermore, transgenic line 3 (ABRL3) showed maximum phenolics and flavonoids content among all tested plant extracts. ABRL3 also demonstrated the highest total antioxidant capacity (8.16 ± 1 μg AAE/mg), total reducing power, (6.60 ± 1.17 μg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8 μg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 μg/mL), and iron-chelating power (IC50 = 154.8 ± 2 μg/mL). Moreover, transformed plant extracts produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant activities in BALB/c mice models. In conclusion, transgenic regenerants of A. bracteosa pose better antioxidant and pharmacological properties under the effect of rol genes as compared to wild-type plants.

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Section: Introductionsupporting

confidence: 70%

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

et al. 2021

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“…RP-HPLC delineated a marked surge in polyphenols of transformed plants than their untransformed counterparts. These differences support our previous ndings indicating that rolABC genes enhanced the expression of biosynthetic pathway genes in transformed regenerants of A. bracteosa [15]. We found that ABRL3 presented highest expression of β-hydroxy β-methylglutaryl-CoA reductase (HMGR), farnesyl diphosphate synthase (FDS), 4-hydroxy-3-methyl-but-2-enyl pyrophosphate synthase (HDS), and phenylalanine ammonia lyase (PAL) genes owing to signi cant phenolic content and antioxidant activity of this line.…”

Section: Discussionsupporting

confidence: 92%

“…Multiple in vivo activities including analgesic, anti-in ammatory, antidepressant, and anticoagulant activity were evaluated using BALB/c mice. In the continuation of our previous study [15], estimation of essential elements of transformed regenerated plants of A. bracteosa by atomic absorption spectrophotometry and HPLC ngerprinting of polyphenol content was performed to support our results.…”

Section: Introductionsupporting

confidence: 67%

“…Intact plants were regenerated from hairy roots by following the previously optimized method in our lab [16]. Polymerase chain reaction (PCR) was performed for the con rmation of transformation and stable integration of rol genes in transformed roots and regenerants [15,16]. Untransformed tissue cultured plants and transgenic regenerants of A. bracteosa plants were used in this study.…”

Section: Source Of Plantmentioning

confidence: 99%

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Ajuga Bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Rubnawaz¹,

Kayani²,

Akhtar³

et al. 2021

Preprint

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Ajuga bracteosa Wall. ex Benth is an endangered medicinal herb used against different ailments in folklore medicines. Here, we aimed to create a new insight to the fundamental mechanisms of genetic transformation in the ethnomedicinal usage of this plant. We transformed the plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. The transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, and linked the activities with elemental analysis, polyphenol content and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was found most abundant in all transgenic lines (up to 101.26 ± 6 µg/mg). Furthermore, among all tested plant extracts, transgenic line 3 (ABRL3) showed maximum phenolics (13.39 ± 2µg GAE/mg) and flavonoids content (4.75 ± 0.16 µg QE/mg). ABRL3 also demonstrated potent total antioxidant capacity (8.16 ± 1 µg AAE/mg), total reducing power, (6.60 ± 1.17 µg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8µg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 µg/mL), and iron chelating power (IC50 = 154.8 ± 2 µg/mL) among all plants. Transformed plant extracts also produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant properties in in vivo mice model as compared to control untransformed plant material. Additionally, no abnormal behavior or lethality was observed in any animal tested. In conclusion, transgenic regenerants of A. bracteosa pose better pharmacological properties under the effect of rol genes as compared to wild type plants.

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“…Salinity can cause osmotic and ionic imbalance, functional and structural protein damage and membrane injury in plants. However, plants can promote some tolerance mechanism linked with signal transduction, reactive oxygen species (ROS) production, induction of antioxidants, synthesizing of osmoprotectants, controlling ion absorption, and expression of salt responsive genes and transcription factor to cope with salinity (Gupta and Huang, 2014;Mishra and Tanna, 2017;Rubnawaz et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Concomitant accumulations of ions, osmoprotectants and antioxidant system-related substances provide salt tolerance capability to succulent extreme-halophyte Scorzonera hieraciifolia

Altuntaş¹,

Terzi²

2021

Turk J Bot

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Halophytes adapting to live in salinized areas can activate some tolerance mechanism through signal compounds to cope with salinity. However, the role of co-activity of signal compounds in salt tolerance of halophytes has not yet fully understood. We researched the changes in signal compounds involved in the salt tolerance mechanism, including inorganic ions, osmoprotectants and substances related to antioxidant system in Scorzonera hieraciifolia with fleshy shoots extremehalophyte. The levels of calcium, magnesium, proline, soluble sugar, hydrogen peroxide, superoxide, ascorbate and glutathione increased when thickness of shoot tissues enhanced under excess salinity. There were 3.3-fold, 5-fold, 8-fold and 10-fold enhancements in the levels of inorganic ions (Ca 2+ and Mg 2+ ), H 2 O 2 , ascorbate and glutathione in the shoots treated with excess salinity, respectively. Contents of sodium, potassium and chlorine, and antioxidant enzyme activities also increased in the salinized shoots. The increases in antioxidant enzyme activities were consistent with increases in their protein contents according to Western blot analysis. The results suggest that extraordinary salt tolerance capacity in Scorzonera hieraciifolia can improve by modulated accumulations of signal compounds, especially calcium, magnesium, osmoprotectants, reactive oxygen species and antioxidant substances. Moreover, massive induction of antioxidant enzymes can make strong contributions to salinity tolerance of S. hieraciifolia.

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Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways

Cited by 5 publications

References 52 publications

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Ajuga Bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Concomitant accumulations of ions, osmoprotectants and antioxidant system-related substances provide salt tolerance capability to succulent extreme-halophyte Scorzonera hieraciifolia

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