Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Rubnawaz, Samina; Akhtar, Nosheen; Mahmood, Rashid; Khan, Asif; Okla, Mohammad K.; Alamri, Saud; Alaraidh, Ibrahim A.; Alwasel, Yasmeen A.; Mirza, Bushra

doi:10.3390/molecules26164874

Cited by 4 publications

(1 citation statement)

References 49 publications

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“…Previously, we found enhanced expression of metabolic pathway genes under the influence of rolABC in A. bracteosa regenerated plants [35]. This enhanced expression can be linked with significantly higher amounts of several polyphenols, as shown in our previous study [36]. Here, among all the tested plants, ABRL3 displayed the highest phenolic content, flavonoid content, antioxidant capacity, radical scavenging, and metal chelating power.…”

Section: Discussionsupporting

confidence: 79%

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants

Rubnawaz

Okla

Akhtar

et al. 2021

Plants

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Herbal and traditional medicines can play a pivotal role in combating cancer and neglected tropical diseases. Ajuga bracteosa, family Lamiaceae, is an important medicinal plant. The genetic transformation of A. bracteosa with rol genes of Agrobacterium rhizogenes further enhances its metabolic content. This study aimed at undertaking the molecular, phytochemical, and in vitro biological analysis of A. bracteosa extracts. We transformed the A. bracteosa plant with rol genes and raised the regenerants from the hairy roots. Transgenic integration and expression of rolB were confirmed by conventional polymerase chain reaction (PCR) and qPCR analysis. The methanol: chloroform crude extracts of wild-type plants and transgenic regenerants were screened for in vitro antibacterial, antihemolytic, cytotoxic, anticancer, and leishmanial activity. Among all plants, transgenic line 3 (ABRL3) showed the highest expression of the rolB gene. Fourier transform infra-red (FTIR) analysis confirmed the enhanced number of functional groups of active compounds in all transgenic lines. Moreover, ABRL3 exhibited the highest antibacterial activity, minimum hemolytic activity (CC50 = 7293.05 ± 7 μg/mL) and maximum antileishmanial activity (IC50 of 56.16 ± 2 μg/mL). ABRL1 demonstrated the most prominent brine shrimp cytotoxicity (LD5039.6 ± 4 μg/mL). ABRL3 was most effective against various human cancer cell lines with an IC50 of 57.1 ± 2.2 μg/mL, 46.2 ± 1.1 μg/mL, 72.4 ± 1.3 μg/mL, 73.3 ± 2.1 μg/mL, 98.7 ± 1.6 μg/mL, and 97.1 ± 2.5 μg/mL against HepG2, LM3, A549, HT29, MCF-7, and MDA-MB-231, respectively. Overall, these transgenic extracts may offer a cheaper therapeutic source than the more expensive synthetic drugs.

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Section: Discussionsupporting

confidence: 79%

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants

Rubnawaz

Okla

Akhtar

et al. 2021

Plants

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Potential Herbal Remedies for Treatment of Depression: A Mini Review

Yadav,

Rani,

Dua

2024

NPJ

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Background: Depression is a psychiatric and mood disorder that impacts a person's mental health and behavior and is frequently linked to suicide. As per the World Health Organization's estimate, depressive disorder will be the main cause of mental disorders by the year 2030, and it has a huge impact on the burden of disease in the world. To treat depression, there are pharmacological and nonpharmacological therapy alternatives. With little to no consideration of other neurochemicals altered in depression, most antidepressant preparations are based on the monoamines, neuroendocrine, and neuro-inflammation concepts. Objective: The present study aims to provide comprehensive data related to depression, the factors associated, the mechanism involved, herbal plants effective for managing depression, and novel formulations along with patents and clinical trials. Methods: A thorough assessment of herbs and novel formulations that have been proven effective in treating depression was conducted. After extensive review, the present study includes a mechanism of action of herbal plants showing antidepressant effects, novel formulations, patents, and clinical trials related to depression. Results: Numerous studies reported that diverse herbal plants have been found to have a positive effect on depression management, such as Panax ginseng, Melissa officinalis, Piper methysticum, Schinus molle L, Kielmeyera coriacea Mart, Elaeocarpus ganitrus, Hypericum perforatum, Lavandula angustifolia Mill, Crocus Sativus L. Conclusion: Herbal plant research could help establish the potential of isolated compounds from plants with medicinal properties for managing depressive illnesses.

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Correction: Rubnawaz et al. Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential. Molecules 2021, 26, 4874

et al. 2022

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Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Cited by 4 publications

References 49 publications

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants

Potential Herbal Remedies for Treatment of Depression: A Mini Review

Correction: Rubnawaz et al. Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential. Molecules 2021, 26, 4874

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