Enhanced steroidogenic and altered antioxidant response by ZnO nanoparticles in mouse testis Leydig cells

Bara, Nisha; Kaul, Gautam

doi:10.1177/0748233718774220

Cited by 28 publications

(33 citation statements)

References 69 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to Sertoli cells, Leydig cells play an important role in maintaining spermatogenesis and are prone to being affected by various chemicals [23,24]. ZnO NPs have been reported to exert cytotoxic effects on mouse Leydig cells [25,26]. Similar toxic effects were revealed in the testis of six-month-old common carp Cyprinus carpio after exposure to 10, 50, and 100 μg/L ZnO NPs for 21 days [27].…”

Section: Introductionmentioning

confidence: 98%

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Shen

Yang

Zhou

et al. 2019

IJMS

View full text Add to dashboard Cite

Zinc oxide nanoparticles (ZnO NPs) have shown adverse health impact on the human male reproductive system, with evidence of inducing apoptosis. However, whether or not ZnO NPs could promote autophagy, and the possible role of autophagy in the progress of apoptosis, remain unclear. In the current study, in vitro and in vivo toxicological responses of ZnO NPs were explored by using a mouse model and mouse Leydig cell line. It was found that intragastrical exposure of ZnO NPs to mice for 28 days at the concentrations of 100, 200, and 400 mg/kg/day disrupted the seminiferous epithelium of the testis and decreased the sperm density in the epididymis. Furthermore, serum testosterone levels were markedly reduced. The induction of apoptosis and autophagy in the testis tissues was disclosed by up-regulating the protein levels of cleaved Caspase-8, cleaved Caspase-3, Bax, LC3-II, Atg 5, and Beclin 1, accompanied by down-regulation of Bcl 2. In vitro tests showed that ZnO NPs could induce apoptosis and autophagy with the generation of oxidative stress. Specific inhibition of autophagy pathway significantly decreased the cell viability and up-regulated the apoptosis level in mouse Leydig TM3 cells. In summary, ZnO NPs can induce apoptosis and autophagy via oxidative stress, and autophagy might play a protective role in ZnO NPs-induced apoptosis of mouse Leydig cells.

show abstract

Section: Introductionmentioning

confidence: 98%

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Shen

Yang

Zhou

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Nanomaterial toxic effects to testicular cells have been reported. Bara and Kaul reported that ZnO nanoparticles affected steroidogenesis and provoked alterations in phagosomes and lysosomes in Leydig cells [25] . Habas et al found that silver-nanoparticles were able to enter sertoli cells and induce apoptosis and oxidative damage altering molecular pathways associated with that process [26] .…”

Section: Resultsmentioning

confidence: 99%

Disposition and Biocompatibility of Dextrin-coated Cadmium Sulphide Nanoparticles after a Single Dose and Multiple Doses in Rats

Cruz¹,

Gómez-Cansino²,

Rodríguez-Fragoso³

et al. 2019

ijps

View full text Add to dashboard Cite

The development of nanomaterials has increased dramatically during the past decade [1] . However, currently only very few studies focus on the disposition of nanomaterials in vivo as well as the time taken to completely eliminate them in the organisms [2,3] . Researchers intended to study nanomaterial biocompatibility need to understand what happens to these after administered to living organisms. Predicting disposition of a nanomaterial is very important in the process of developing new nanomaterials for theranostic use in human beings [4] . All preclinical studies on nanomaterials should require a pharmacokinetic study in order to predict and understand the disposition once the nanomaterial enters the organism, when it is finally completely eliminated, and how safe its use is overall. The fate and disposition of any nanomaterial should be based on its properties, site of administration, formulation, and dosage [5] . For this reason, it is necessary to explore biocompatibility, tolerance and characterize the pharmacokinetic profile of each synthetized nanomaterial.An important parameter to consider in pharmacokinetic studies is the estimated mean time a drug resides in the body within specific tissues; the mean residence time (MRT) can help assess the extent of its efficacy and

show abstract

“…Recently, Bara and his co-authors 35 Although several literature discusses the protective effect of ZnO NPS against drugs or toxic substances, to date, the molecular mechanism of ZnO NPs is still absence, especially in vivo.…”

Section: Discussionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Improve Testicular Steroidogenesis Machinery Dysfunction In Benzo [α] Pyrene Challenged Rats

Daoud

Mohamed

Ezzo

et al. 2021

Preprint

View full text Add to dashboard Cite

Although Zinc oxide nanoparticles (ZnO NPs) in low doses have potentially positive effects on reproduction by their antioxidant effects, the defensive role of Zinc nanomaterials against environmental pollutants that affect male reproduction has not been adequately studied. We designed our study to assess the impact of ZnO NPs towards reproductive dysfunction induced by Benzo[α]Pyrene (B[a]P). Forty-eight mature male rats were randomly distributed into six equal groups: G1; negative control, G2&3- positive control I &II (either 10 or 30 mg ZnO NPs / kg BW); G4. (150 mg Bap / kg BW), G 5 & 6 (Co- administrated B[a]P with different concentrations of ZnO NPs). Oxidative stress biomarkers, semiquantitative real-time PCR for steroidogenic enzymes (CY11A1, StAR, and 3β- HSD), testosterone levels and histopathology in the liver, kidney, and testicles were examined for this investigation. B[a] P treated group showed significant deterioration in all reproductive parameters and induced oxidative stress. Co-administration ZnO NPs eased oxidative stress and effectively increased the expression of CY11A1, StAR, and 3β- HSD and improved histopathological changes in the examined organs. Our results using the selected doses and with Nano particle properties confirm that ZnO NPs have an obvious ameliorative effect against B[a] P.

show abstract

Enhanced steroidogenic and altered antioxidant response by ZnO nanoparticles in mouse testis Leydig cells

Cited by 28 publications

References 69 publications

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Disposition and Biocompatibility of Dextrin-coated Cadmium Sulphide Nanoparticles after a Single Dose and Multiple Doses in Rats

Zinc Oxide Nanoparticles Improve Testicular Steroidogenesis Machinery Dysfunction In Benzo [α] Pyrene Challenged Rats

Contact Info

Product

Resources

About