Enhanced Sensitivity of Nucleoli in Human Proliferating Cells to Inhibition of Protein Synthesis with Anisomycin

Abstract

We describe the reaction of nuclei in cultured human cells from different tissues to inhibition of total protein synthesis with anisomycin - ribotoxin, which is now considered as a potential antitumor drug. It was shown that nucleoli in sensitive cells demonstrate typical reaction: under the action of the inhibitor, labile nucleolar protein, a component of RNA polymerase I transcription complex (previously called A3 antigen), rapidly migrates from the nucleolus to numerous discrete foci in the nucleoplasm. These changes are specific for translation suppression and are not induced by other influences on the cells. Migration of A3 antigen into the nucleoplasm manifests primarily in cells at the stage of DNA replication and is absent in resting cells. These results suggest that localization of A3 antigen can be a marker of artificial suppression of translation in proliferating human cells in vitro.

Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit in vitro

“…Anisomycin is also well known as a protein synthesis inhibitor that is involved in apoptosis [7][8][9] . In this study, we investigated the effects of anisomycin on U251 and U87 human glioblastoma cells.…”