Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor

Abstract

Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis. To transform the fabricated devices as a functional sensing element, three-step procedure consist of SiNW surface modification, DNA immobilization and DNA hybridization were employed. The detection principle works by detecting the changes in current of SiNW which bridge the source and drain terminal to sense the immobilization of probe DNA and their hybridization with target DNA. The oxygen (O) plasma was proposed as an effective strategy for increasing the binding amounts of target DNA by modified the SiNW surface. It was found that the detection limit of the optimized O plasma treated-SiNW device could be reduced to 1.985 × 10 M with a linear detection range of the sequence-specific DNA from 1.0 × 10 M to 1.0 × 10 M. In addition, the developed biosensor device was able to discriminate between complementary, single mismatch and non-complementary DNA sequences. This highly sensitive assay was then applied to the detection of reverse transcription-polymerase chain reaction (RT-PCR) product of DENV-DNA, making it as a potential method for disease diagnosis through electrical biosensor.

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

“…Meanwhile, merely 0.7% current change was recorded when 20 ng/mL of CFP10 was introduced. The limit of detection (LOD) can be defined as Y LOD = Y probe + 3σ; where Y probe is the mean of the probe measurement and σ is the standard deviation of the calibration curve [ 34 , 35 ]. The linear regression equation of the plots shown in Figure 6 b is Y LOD = 5.7 log 10 x + 44.65, where x is the concentration of target CFP10 antigen and Y LOD is calculated from the equation.…”

Surface Enhanced CdSe/ZnS QD/SiNP Electrochemical Immunosensor for the Detection of Mycobacterium Tuberculosis by Combination of CFP10-ESAT6 for Better Diagnostic Specificity

“…The measurement of the test was repeated three times for every concentration of the CFP10-ESAT6. The detection response of the developed sensor can be expressed as the relative change in current (∆I/I 0 ), where ∆I is the change in peak current after the target detection and I 0 is baseline current before detection [43]. The limit of detection (LOD) was calculated using the 3 sigma method; Y (LOD) = 3σ, where σ is the residual standard deviation [44].…”

3D Hybrid Small Scale Devices

“…Small structures such as nanorods, nanowires, and the use of nano/micro topography have made it possible to probe physical phenomena at cellular, subcellular organelle, and molecular levels . Indeed, crafting probes at this length scale enables not just recapitulation and measurement of cellular and molecular processes, but also infiltration into nuclei and manipulation of gene transcription and other subcellular processes .…”