Enhanced resistance to Sendai virus infection in DBA/2J mice with a botanical drug combination (Sinupret®)

Schmolz, Manfred; Ottendorfer, D.; März, RW; Sieder, C

doi:10.1016/s1567-5769(01)00108-4

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…The drug has been extensively investigated in a variety of scientific studies and clinical settings. Prophylactic administration to mice led to increased resistance against respiratory tract infection following intranasal application of Sendai virus (Parainfluenza viridae) [30]. Mucus clearance properties have been observed in rabbit tracheas in vivo [31].…”

Section: Discussionmentioning

confidence: 99%

Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance

et al. 2014

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IntroductionWe have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl−) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this bioflavonoid.MethodsPrimary murine nasal septal epithelial (MNSE) [wild type (WT) and transgenic CFTR−/−], human sinonasal epithelial (HSNE), WT CFTR-expressing CFBE and TMEM16A-expressing HEK cultures were utilized for the present experiments. CBF and ASL depth measurements were performed. Mechanisms underlying transepithelial Cl− transport were determined using pharmacologic manipulation in Ussing chambers, Fura-2 intracellular calcium [Ca2+]i imaging, cAMP signaling, regulatory domain (R-D) phosphorylation of CFTR, and excised inside out and whole cell patch clamp analysis.ResultsSinupret-mediated Cl− secretion [ΔISC(µA/cm2)] was pronounced in WT MNSE (20.7+/−0.9 vs. 5.6+/−0.9(control), p<0.05), CFTR−/− MNSE (10.1+/−1.0 vs. 0.9+/−0.3(control), p<0.05) and HSNE (20.7+/−0.3 vs. 6.4+/−0.9(control), p<0.05). The formulation activated Ca2+ signaling and TMEM16A channels, but also increased CFTR channel open probability (Po) without stimulating PKA-dependent pathways responsible for phosphorylation of the CFTR R-domain and resultant Cl− secretion. Sinupret also enhanced CBF and ASL depth.ConclusionSinupret stimulates CBF, promotes transepithelial Cl− secretion, and increases ASL depth in a manner likely to enhance MCC. Our findings suggest that direct stimulation of CFTR, together with activation of Ca2+-dependent TMEM16A secretion account for the majority of anion transport attributable to Sinupret. These studies provide further rationale for using robust Cl− secretagogue based therapies as an emerging treatment modality for common respiratory diseases of MCC including acute and chronic bronchitis and CRS.

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Section: Discussionmentioning

confidence: 99%

Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance

et al. 2014

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“…14 Experimental studies have demonstrated that Sinupret and its individual components have mucolytic properties, 15 and prophylactic administration increases resistance to respiratory tract infection by intranasal application of Sendai virus (Parainfluenza viridae) in mice. 16 Sinupret also has demonstrated efficacy in several clinical studies of rhinosinusitis. A randomized, placebo-controlled trial involving 31 patients 17 with rhinosinusitis demonstrated significant improvement in radiologic outcomes and headache in patients receiving Sinupret alone.…”

Section: Fig 1 Sinupret Stimulates Transepithelial CLmentioning

confidence: 99%

The bioflavonoid compound, sinupret, stimulates transepithelial chloride transport in vitro and in vivo

et al. 2010

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“…extracts of Verbena herb and Primula flower, reduced the spreading of three viruses (influenza A, respiratory syncytial virus and parainfluenza type 1). An antiviral effect of Sinupret ® in vivo could be demonstrated in a murine virus model (Sendai virus), in which the survival time of mice could be increased by Sinupret ® tablets therapeutically (März et al 1999) and by Sinupret ® drops if given prophylactically (Schmolz et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity in vitro of two preparations of the herbal medicinal product Sinupret® against viruses causing respiratory infections

et al. 2011

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Sinupret(®), a herbal medicinal product made from Gentian root, Primula flower, Elder flower, Sorrel herb, and Verbena herb is frequently used in the treatment of acute and chronic rhinosinusitis and respiratory viral infections such as common cold. To date little is known about its potential antiviral activity. Therefore experiments have been performed to measure the antiviral activity of Sinupret(®) oral drops (hereinafter referred to as "oral drops") and Sinupret(®) dry extract (hereinafter referred to as "dry extract"), in vitro against a broad panel of both enveloped and non-enveloped human pathogenic RNA and DNA viruses known to cause infections of the upper respiratory tract: influenza A, Chile 1/83 (H1N1) virus (FluA), Porcine Influenza A/California/07/2009 (H1N1) virus (pFluA), parainfluenza type 3 virus (Para 3), respiratory syncytial virus, strain Long (RSV), human rhinovirus B subtype 14 (HRV 14), coxsackievirus subtype A9 (CA9), and adenovirus C subtype 5 (Adeno 5). Concentration-dependent antiviral activity (EC(50) between 13.8 and 124.8 μg/ml) of Sinupret(®) was observed against RNA as well as DNA viruses independent of a viral envelope. Remarkable antiviral activity was shown against Adeno 5, HRV 14 and RSV in which dry extract was significantly superior to oral drops. This could be ascertained with different assays as plaque-reduction assays in plaque forming units (PFU), the analyses of a cytopathogenic effect (CPE) and with enzyme immunoassays (ELISA) to determine the amount of newly synthesised virus. Our results demonstrate that Sinupret(®) shows a broad spectrum of antiviral activity in vitro against viruses commonly known to cause respiratory infections.

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Enhanced resistance to Sendai virus infection in DBA/2J mice with a botanical drug combination (Sinupret®)

Cited by 7 publications

References 7 publications

Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance

Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance

The bioflavonoid compound, sinupret, stimulates transepithelial chloride transport in vitro and in vivo

Antiviral activity in vitro of two preparations of the herbal medicinal product Sinupret® against viruses causing respiratory infections

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