Enhanced removal of ethidium bromide (EtBr) from aqueous solution using rectorite

In silico recombinant plasmid design of pHA171 with phdABCD insertion for ethidium bromide degradation

“…Improper disposal of ethidium bromide has the potential to contaminate groundwater which may be used for parks, agriculture, washing or even drinking purposes. Because ethidium bromide is a potent mutagen, high priority should be put to manage the waste containing that compound [3]. Therefore, one alternatif to overcome the problem is through a bioremediation strategy.…”

Mechanistic insights into ethidium bromide removal by palygorskite from contaminated water

“…Therefore, there is a certain hazard to the operator and the laboratory environment from EtBr, and harmless treatment of the compound is an important task in the laboratory. Additionally, if EtBr-containing wastewater is deliberately or accidentally released into the soil or water bodies, that may cause a tremendous hazard to biodiversity because EtBr can mutate living microbial cells and pose toxicity to higher organisms (Li et al, 2020). Removal of EtBr from laboratory wastewater is therefore a matter of immense importance.…”

“…Depending on the type, composition and modification of clay minerals, they remove contaminants from water by adsorption, catalytic degradation, or redox-transformation (Zhu et al, 2016). Among the above removal methods, adsorption has been found as the most popular one because of its easy operational requirements, and relatively higher contaminant removal efficiency compared to other methods without much investment for complex modification of the adsorbent or requirement for additional chemicals (Chang et al, 2012(Chang et al, , 2014(Chang et al, , 2016Li et al, 2020).…”

“…Although clay minerals have been applied for removing numerous organic and inorganic contaminants in aqueous media, reports on their use for the adsorptive removal of EtBr from water has been limited in the literature. EtBr adsorption by swelling and mixed-layer clay mineral such as rectorite was recently studied (Li et al, 2020), and the results showed that EtBr could be intercalated in the interlayers of rectorite. Additionally, bromide ions present in EtBr molecules played a significant role in the formation of dimers which enhanced the EtBr adsorption capacity of the clay mineral (Li et al, 2020).…”

“…EtBr adsorption by swelling and mixed-layer clay mineral such as rectorite was recently studied (Li et al, 2020), and the results showed that EtBr could be intercalated in the interlayers of rectorite. Additionally, bromide ions present in EtBr molecules played a significant role in the formation of dimers which enhanced the EtBr adsorption capacity of the clay mineral (Li et al, 2020). The authors reported that the maximum EtBr adsorption capacity by rectorite was 400 mmol/kg corresponding to 160 mg/g at pH 4.6, and the ratio of solid/solution was 0.2 g/20 mL (Li et al, 2020).…”