Enhanced protein degradation by black soldier fly larvae (Hermetia illucens L.) and its gut microbes

Yu, Yongqiang; Zhang, Jia; Zhu, Fengling; Fan, Mingxia; Zheng, Jinshui; Cai, Minmin; Zheng, Longyu; Huang, Feng; Zhou, Yu; Zhang, Jibin

doi:10.3389/fmicb.2022.1095025

Cited by 22 publications

(19 citation statements)

References 61 publications

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“…Furthermore, the gut microbiota and the enzymes they secrete do more than just break down harmful compounds and macromolecules; they also suppress infections, preparing the substrate for insects [ 47 ]. Research by Yu et al into the gut microbes’ role in protein degradation by BSFL (germ-free or gnotobiotic) revealed the gnotobiotic treatment had a significantly higher protein reduction rate than germ-free treatment [ 48 ] Thus, the differences of microbiota between treatments in our study might also impact the process of the manure conversion with BSFL. However, the precise correlation between the gut microbiota and digestion needs to be researched in depth.…”

Section: Discussionmentioning

confidence: 81%

Effects of Disinfectants on Larval Growth and Gut Microbial Communities of Black Soldier Fly Larvae

Hao

Liu

Guo

et al. 2023

Insects

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The use of the black soldier fly has been demonstrated to be effective in the treatment of swine manure. Since the outbreaks of ASFV, prevention procedures, including manure disinfection, have changed dramatically. Glutaraldehyde (GA) and potassium peroxymonosulfate (PPMS) have been shown to be effective in the prevention of this pathogen and are thus widely used in the disinfection of swine manures, etc. However, research on the effects of disinfectants in manures on the growth of BSFL and gut microbiota is scarce. The goal of this study was to determine the effects of GA and PPMS on BSFL growth, manure reduction, and gut microbiota. In triplicate, 100 larvae were inoculated in 100 g of each type of manure compound (manure containing 1% GA treatment (GT1), manure containing 0.5% GA treatment (GT2), manure containing 1% PPMS treatment (PT1), manure containing 0.5% PPMS treatment (PT2), and manure without disinfectant (control)). After calculating the larval weight and waste reduction, the larval gut was extracted and used to determine the microbial composition. According to the results, the dry weights of the larvae fed PT1–2 (PT1: 86.7 ± 4.2 mg and PT2: 85.3 ± 1.3 mg) were significantly higher than those of the larvae fed GT1–2 (GT1: 72.5 ± 2.1 mg and GT2: 70 ± 2.8 mg) and the control (64.2 ± 5.8 mg). There was a 2.8–4.03% higher waste reduction in PT1–2 than in the control, and the waste reduction in GT1–2 was 7.17–7.87% lower than that in the control. In a gut microbiota analysis, two new genera (Fluviicola and Fusobacterium) were discovered in PT1–2 when compared to GT1–2 and the control. Furthermore, the disinfectants did not reduce the diversity of the microbial community; rather, Shannon indices revealed that the diversities of GT1–2 (GT1: 1.924 ± 0.015; GT2: 1.944 ± 0.016) and PT1 (1.861 ± 0.016) were higher than those of the control (1.738 ± 0.015). Finally, it was found that both disinfectants in swine manures at concentrations of 1% and 0.5% may be beneficial to the complexity and cooperation of BSFL gut microbiota, according to an analysis of microbial interactions.

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Section: Discussionmentioning

confidence: 81%

Effects of Disinfectants on Larval Growth and Gut Microbial Communities of Black Soldier Fly Larvae

Hao

Liu

Guo

et al. 2023

Insects

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“…In another study, it found that protein decomposing bacteria of intestinal bacteria of Gnotobiotic BSFL created by incubating germ free BSFL with BSFL gut fed on a high-protein artificial diets were diverse including Pseudomonas spp., Orbus spp., Campylobacter spp., Dysgonomonas spp., Issatchenkia spp., Campylobacter spp., Pediococcus spp., Lactobacillus spp., Lactobacillus spp., and Bacillus spp., and Issatchenkia spp. [9].…”

Section: Identification Results Of Selected Bacteriamentioning

confidence: 99%

“…However, the microbiota composition of sample types could be different [4]. Researches of black soldier fly larvae gut microflora included antibacterial peptide active substances extraction [5], intestinal specific microbiota (Xie ei al., 2010) [6], conserved microbiota analysis [7], active enzymes analysis and identification [8], enhanced protein degradation by BSF and its gut microbes [9]. Most of researches of the BSFL gut microflora involved metagenomics, culture-independent molecular methods while studies by using culture-dependent methods provided direct evidences of the characteristics and roles of on BSF microflora were limited.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of bacteria with capability of starch, cellulose and protein decomposition from Frass of Black Soldier Fly (Hermetia Illucens) Larvae

Tu¹,

An²,

Ngan³

et al. 2023

World J. Adv. Res. Rev.

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This research isolated and identified bacteria with capability of starch, cellulose and protein decomposition from frass of black soldier fly (Hermetia Illucens) larvae (BFL). Dilute suspension of BFL frass was spread on petri dishes with solid Nutrient Agar for bacteria isolation. Selection and evaluation of bacteria with capability of starch, cellulose and protein decomposition was based on their ability of creating halo zone on starch agar, CMC and skim milk media. Identification of selected bacterial isolates was based on 16S rDNA sequences. There were 44 bacterial strains isolated from BSFL frass. Bacterial strains with the best capability of starch, cellulose and protein decomposition were CCRL21 and CCRL16 with halo zone radii of 1.5 cm and 0.8 cm; CCRL16, CCRL20 with halo zone radii with the similar size of 0.83 cm; and CCRL44 has the best ability of protein decomposition with an average halo zone radius about 2.47 cm, respectively. Six bacterial strains with good ability of degrading cellulose, starch and protein were identified as Bacillus paramycoides CCRL1; Bacillus altitudinis CCRL16, Bacillus spizizenii CCRL20, Bacillus altitudinis CCRL21, Lysinibacillus pakistanensis CCRL32 and Paenibacillus silvae CCRL43.

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“…The larval gut microbiota contributes considerably to the decomposition of agri-FW. Due to the diverse range of microbial and enzymatic activity found in the digestive system of BSFL, it is considered one of the most effective in breaking down food waste and other organic materials [ 122 , 123 ]. Recent metagenomic studies have provided microbiological evidence to support the degradation efficiency of BSFL.…”

Section: Agri-food Waste Utilizing Insects and Gut Symbiotic Microbiotamentioning

confidence: 99%

Insect-based agri-food waste valorization: Agricultural applications and roles of insect gut microbiota

Mannaa

Mansour

Park

et al. 2024

Environmental Science and Ecotechnology

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Enhanced protein degradation by black soldier fly larvae (Hermetia illucens L.) and its gut microbes

Cited by 22 publications

References 61 publications

Effects of Disinfectants on Larval Growth and Gut Microbial Communities of Black Soldier Fly Larvae

Effects of Disinfectants on Larval Growth and Gut Microbial Communities of Black Soldier Fly Larvae

Isolation and identification of bacteria with capability of starch, cellulose and protein decomposition from Frass of Black Soldier Fly (Hermetia Illucens) Larvae

Insect-based agri-food waste valorization: Agricultural applications and roles of insect gut microbiota

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