Enhanced Protection of Biological Membranes during Lipid Peroxidation: Study of the Interactions between Flavonoid Loaded Mesoporous Silica Nanoparticles and Model Cell Membranes

Abstract

Flavonoids, polyphenols with anti-oxidative activity have high potential as novel therapeutics for neurodegenerative disease, but their applicability is rendered by their poor water solubility and chemical instability under physiological conditions. In this study, this is overcome by delivering flavonoids to model cell membranes (unsaturated DOPC) using prepared and characterized biodegradable mesoporous silica nanoparticles, MSNs. Quercetin, myricetin and myricitrin have been investigated in order to determine the relationship between flavonoid structure and protective activity towards oxidative stress, i.e., lipid peroxidation induced by the addition of hydrogen peroxide and/or Cu2+ ions. Among investigated flavonoids, quercetin showed the most enhanced and prolonged protective anti-oxidative activity. The nanomechanical (Young modulus) measurement of the MSNs treated DOPC membranes during lipid peroxidation confirmed attenuated membrane damage. By applying a combination of experimental techniques (atomic force microscopy—AFM, force spectroscopy, electrophoretic light scattering—ES and dynamic light scattering—DLS), this work generated detailed knowledge about the effects of flavonoid loaded MSNs on the elasticity of model membranes, especially under oxidative stress conditions. Results from this study will pave the way towards the development of innovative and improved markers for oxidative stress-associated neurological disorders. In addition, the obtained could be extended to designing effective delivery systems of other high potential bioactive molecules with an aim to improve human health in general.

Tea polyphenols alleviate tri‐ortho‐cresyl phosphate‐induced autophagy of mouse ovarian granulosa cells

“…ROS are shown to peroxidize lipids such as polyunsaturated fatty acids (PUFAs), which finally form malondialdehyde (MDA). The particular reaction of ROS with lipids is generally known as “lipid peroxidation.” In this study, we showed that the TOCP exposure led to a significant increase of malondialdehyde (MDA), accompanying with remarkable plunge of GSH levels and impaired enzymatic activity of SOD and GSH‐PX in the mouse ovarian granulosa cells. There was also a significant increase in intracellular ROS level in the TOCP‐treated cells.…”

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

“…The antioxidant activity of flavonoids is directly proportional to the number and position of hydroxyl groups in their structure [97], that assist in the dissipation of electrons generated after UV-overexposure [98]. Additionally, they also prevent lipid peroxidation [99] (by decomposing lipid peroxides and scavenging harmful free-radicals) and develop an effective metal chelation activity [100]. The free-radical scavenging [62,101,102] and lipid oxidation preventing activities [64] of Bryophyllum-derived extracts rich in flavonoids have already been reported.…”

The Fine-Tuned Release of Antioxidant from Superparamagnetic Nanocarriers under the Combination of Stationary and Alternating Magnetic Fields

“…It is an important component of the human’s daily diet and widely distributed in vegetables and fruits such as onions, tomatoes, berries, grapes, nuts, as well as in many flowers and leaves [ 2 , 3 ]. In addition, quercetin exhibits a wide range of biological and pharmacological activities, including antioxidant, anti-inflammatory, antibacterial, anti-anaemic, and anticarcinogenic activities [ 1 , 3 , 4 , 5 ]. Extensive studies have reported that quercetin can inhibit the proliferation of several types of cancers such as lung, prostate, breast cancer, and pancreatic tumour cells [ 1 ].…”