Enhanced Protease Production from Rummeliibacillus Stabekisii (TWSS-P-2) Strain through Mutagenesis and Optimized Culture Conditions

Abstract

Identification of proper microbial sources and optimizing the enzyme production conditions are essential for industrial-scale enzyme production. The present study was done to identify and enhance the production of protease enzyme from an important microbial source Rummeliibacillus stabekisii (TWSS-P-2). Ultra-violet radiation physical method and ethyl methanesulfonate and ethidium bromide dependent chemical methods were considered for mutagenesis. Enzyme assay-dependent screening resulted in identifying Rummeliibacillus stabekisii (TWSS-P-2) as the best strain with optimum protease production that was improved through the chemical treatment mentioned. The strains were tested under various physical and chemical factors including carbon source, nitrogen source, inoculum sizes, pH, temperature to optimize the production of the protein. Submerged fermentation (SmF) was used to assess enzyme production. We were successful in deriving the optimum condition for the protease enzyme production for Rummeliibacillus stabekisii (TWSS-P-2) and the mutagenic effect yielded 2-4 fold better enzyme production.