Enhanced production of <scp>d</scp>‐psicose 3‐epimerase in <i>Bacillus subtilis</i> by regulation of segmented fermentation

Fu, Gang; Zhang, Shibin; Dong, Huina; Chen, Jingqi; Tu, Ran; Zhang, Dawei

doi:10.1002/bab.1831

Cited by 10 publications

(4 citation statements)

References 19 publications

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“…In April 2019, the US Food and Drug Administration (FDA) announced that D-allulose was to be excluded from the total and added sugar contents on nutrition labels when used as an ingredient, thereby greatly expanding its potential market. An efficient enzymic synthesis of D-allulose by D-psicose 3-epimerase (DPEase) catalysis has been attracting strong industrial interest [ 2 ]. However, chemical manufacturing and biosynthetic processes generally occur under harsh conditions, so a cost-effective biocatalytic manufacturing process for D-allulose is not feasible because of the poor thermostability of natural DPEases [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges

Zhao

Chen

Wang

et al. 2021

IJMS

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D-psicose 3-epimerase (DPEase) catalyzes the isomerization of D-fructose to D-psicose (aka D-allulose, a low-calorie sweetener), but its industrial application has been restricted by the poor thermostability of the naturally available enzymes. Computational rational design of disulfide bridges was used to select potential sites in the protein structure of DPEase from Clostridium bolteae to engineer new disulfide bridges. Three mutants were engineered successfully with new disulfide bridges in different locations, increasing their optimum catalytic temperature from 55 to 65 °C, greatly improving their thermal stability and extending their half-lives (t1/2) at 55 °C from 0.37 h to 4–4.5 h, thereby greatly enhancing their potential for industrial application. Molecular dynamics simulation and spatial configuration analysis revealed that introduction of a disulfide bridge modified the protein hydrogen–bond network, rigidified both the local and overall structures of the mutants and decreased the entropy of unfolded protein, thereby enhancing the thermostability of DPEase.

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Section: Introductionmentioning

confidence: 99%

Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges

Zhao

Chen

Wang

et al. 2021

IJMS

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“…During microbial fermentation, pH often affects cell growth and the metabolism of cells (Zou et al, 2014). Based on this fact, Fu et al (2019) proposed a segmented fermentation strategy. A strain of B. subtilis harboring DPE was constructed, and fermentation was performed in stages by adjusting the pH value to achieve a better balance between the growth of B. subtilis and the expression of DPE.…”

Section: Other Strategiesmentioning

confidence: 99%

Bioproduction of D‐allulose: Properties, applications, purification, and future perspectives

Jiang

et al. 2021

Comp Rev Food Sci Food Safe

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D-allulose is the C-3 epimer of D-fructose, which rarely exists in nature, and can be biosynthesized from D-fructose by the catalysis of D-psicose 3-epimerase. Dallulose is safe for human consumption and was recently approved by the United States Food and Drug Administration for food applications. It is not only able be used in food and dietary supplements as a low-calorie sweetener, but also modulates a variety of physiological functions. D-allulose has gained increasing attention owing to its excellent properties. This article presents a review of recent progress on the properties, applications, and bioproduction progress of D-allulose.

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“…In their study, Fu et al. [12] cloned the d ‐Psicose 3‐epimerase (RDPE) from Ruminococcus sp . and expressed it in Bacillus subtilis A311 .…”

Section: The Recovery Of Other Value Products From Biofuels‐producingmentioning

confidence: 99%

Biofuels Production from Renewable Resources

Bao

Catucci

Valetti

2020

Biotech and App Biochem

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Enhanced production of d‐psicose 3‐epimerase in Bacillus subtilis by regulation of segmented fermentation

Cited by 10 publications

References 19 publications

Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges

Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges

Bioproduction of D‐allulose: Properties, applications, purification, and future perspectives

Biofuels Production from Renewable Resources

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