Biofuels Production from Renewable Resources

Bao, Jie; Catucci, Gianluca; Valetti, Francesca

doi:10.1002/bab.2046

Cited by 4 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been found that lipids/fatty acids and their oxidation products are involved in plant responses against diverse stresses, including salt, drought, heavy metal, pathogen infection and other adversities [2][3][4][5][6]. Importantly, vegetable oils are necessary for human health and animal nutrition [7][8][9], also increasing to be as sources of a new generation of environmentally friendly, biodegradable, and renewable industrial products such as biopolymers, biodiesel and high-grade lubricants [10,11]. To meet a growing market demand for vegetable oils globally, it is so much imperative to largely increase vegetable oil yields, which needs to decipher the mechanism responsible for lipid/oil biosynthesis and regulation in oil-rich plants.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa

et al. 2022

View full text Add to dashboard Cite

Background Dof transcription factors (TFs) containing C2-C2 zinc finger domains are plant-specific regulatory proteins, playing crucial roles in a variety of biological processes. However, little is known about Dof in Camelina sativa, an important oil crop worldwide, with high stress tolerance. In this study, a genome-wide characterization of Dof proteins is performed to examine their basic structural characteristics, phylogenetics, expression patterns, and functions to identify the regulatory mechanism underlying lipid/oil accumulation and the candidate Dofs mediating stress resistance regulation in C. sativa. Results Total of 103 CsDof genes unevenly distributed on 20 chromosomes were identified from the C. sativa genome, and they were classified into four groups (A, B, C and D) based on the classification of Arabidopsis Dof gene family. All of the CsDof proteins contained the highly-conserved typic CX2C-X21-CX2C structure. Segmental duplication and purifying selection were detected for CsDof genes. 61 CsDof genes were expressed in multiple tissues, and 20 of them showed tissue-specific expression patterns, suggesting that CsDof genes functioned differentially in different tissues of C. sativa. Remarkably, a set of CsDof members were detected to be possible involved in regulation of oil/lipid biosynthesis in C. sativa. Six CsDof genes exhibited significant expression changes in seedlings under salt stress treatment. Conclusions The present data reveals that segmental duplication is the key force responsible for the expansion of CsDof gene family, and a strong purifying pressure plays a crucial role in CsDofs’ evolution. Several CsDof TFs may mediate lipid metabolism and stress responses in C. sativa. Several CsDof TFs may mediate lipid metabolism and stress responses in C. sativa. Collectively, our findings provide a foundation for deep understanding the roles of CsDofs and genetic improvements of oil yield and salt stress tolerance in this species and the related crops.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa

et al. 2022

View full text Add to dashboard Cite

show abstract

“…26 Among the most used enzymes for dye decolorization and bioremediation are oxidoreductases, such as peroxidases, azoreductases, and laccases. [27][28][29] The microorganisms traditionally used to remove the colorants in textile wastes are the basidiomycete white-rot fungi. 30,31 The fungal species from the Trametes genus produce the ligninolytic enzyme, high redox potential laccase, in high concentrations.…”

Section: Introductionmentioning

confidence: 99%

Modeling and experimental validation of covalent immobilization of Trametes maxima laccase on glyoxyl and MANA‐Sepharose CL 4B supports, for the use in bioconversion of residual colorants

Cutiño-Avila

Sánchez-López

Cárdenas-Moreno

et al. 2021

Biotech and App Biochem

View full text Add to dashboard Cite

Our novel strategy for the rational design of immobilized derivatives (RDID) is directed to predict the behavior of the protein immobilized derivative before its synthesis, by the usage of mathematic algorithms and bioinformatics tools. However, this approach needs to be validated for each target enzyme. The objective of this work was to validate the RDID strategy for covalent immobilization of the enzyme laccase from Trametes maxima MUCL 44155 on glyoxyl‐ and monoaminoethyl‐N‐aminoethyl (MANA)‐Sepharose CL 4B supports. Protein surface clusters, more probable configurations of the protein–supports systems at immobilization pHs, immobilized enzyme activity, and protein load were predicted by RDID1.0 software. Afterward, immobilization was performed and predictions were experimentally confirmed. As a result, the laccase‐MANA‐Sepharose CL 4B immobilized derivative is better than laccase‐glyoxyl‐Sepharose CL 4B in predicted immobilized derivative activity (63.6% vs. 29.5%). Activity prediction was confirmed by an experimentally expressed enzymatic activity of 68%, using 2,6‐dimethoxyphenol as substrate. Experimental maximum protein load matches the estimated value (11.2 ± 1.3 vs. 12.1 protein mg/support mL). The laccase‐MANA‐Sepharose CL 4B biocatalyst has a high specificity for the acid blue 62 colorant. The results obtained in this work suggest the possibility of using this biocatalyst for wastewater treatment.

show abstract

“…stress the potential use of a specific class of enzyme, dye‐decolorizing peroxidases in lignin degradation. Bao et al 4 . focus on four key determining aspects of biofuels production from renewable resources: innovative processes for the production of bioethanol, butanol, and methane; new enzymatic technologies for the modification of lignocellulose matrices; the role of positive and negative modulators on biofuel production; and the recovery of other value‐added products from biofuel‐producing wastes.…”

Section: Introductionmentioning

confidence: 99%

“…Catucci et al 3 stress the potential use of a specific class of enzyme, dye-decolorizing peroxidases in lignin degradation. Bao et al 4 focus on four key determining aspects of biofuels production from renewable resources: innovative processes for the production of bioethanol, butanol, and methane; new enzymatic technologies for the modification of lignocellulose matrices; the role of positive and negative modulators on biofuel production; and the recovery of other value-added products from biofuel-producing wastes. In principle, lignocellulose biochemical conversion includes three major steps: initial physical and chemical pretreatments to destroy wall polymers, sequential enzymatic hydrolysis to release soluble sugars, and final yeast fermentation to produce ethanol.…”

mentioning

confidence: 99%

Comparison of the effects of different pretreatments on the structure and enzymatic hydrolysis of Miscanthus

Dai

Yang

et al. 2021

Biotech and App Biochem

View full text Add to dashboard Cite

Miscanthus is regarded as a desired bioenergy crop with enormous lignocellulose residues for biofuels and other chemical products. In this study, the effect of different pretreatments (including microwave, NaOH, CaO, and microwave + NaOH/CaO) on sugar yields was investigated, leading to largely varied hexose yields at 4.0–73.4% (% cellulose) released from enzymatic hydrolysis of pretreated Miscanthus residues. Among them, the highest yield of 73.4% for hexoses was obtained from 12% NaOH (w/v) solution pretreatment, whereas 1% CaO (w/w) and microwave pretreatment resulted in a lower hexose yield than the control (without pretreatment). The sugar yield from microwave followed with 1% NaOH pretreatment was 4.3 times higher than that of microwave followed with 1% CaO. However, the enzymatic hydrolysis efficiencies of the sample were 15.2% and 58.5% under microwave pretreatment followed by 12% NaOH or 12.5% CaO, respectively, which were lower than those of the same concentration of alkali (NaOH and CaO) pretreatments. To investigate the mechanism of varied enzymatic saccharification under different pretreatments, the changes in the surface structure and porosity of the Miscanthus‐pretreated lignocelluses were studied by means of Fourier transform infrared, Congo red staining, and scanning electron microscopy analysis. The results show that the different pretreatments destroy the cell wall cladding structure and reduce the bonding force between cellulose, hemicellulose, and lignin to different degrees, therefore increasing the accessibility of cellulose and enhancing cellulose digestion.

show abstract

Biofuels Production from Renewable Resources

Cited by 4 publications

References 12 publications

Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa

Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa

Modeling and experimental validation of covalent immobilization of Trametes maxima laccase on glyoxyl and MANA‐Sepharose CL 4B supports, for the use in bioconversion of residual colorants

Comparison of the effects of different pretreatments on the structure and enzymatic hydrolysis of Miscanthus

Contact Info

Product

Resources

About