Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in repeated batch culture with optimized substitution of culture medium

Jin, Il-Hyuck; Jung, Dae-Young; Son, Chang-Woo; Kim, Sung-Koo; Gao, Wa; Chung, Chong-Pyoung; Lee, Jin Woo

doi:10.1007/s12257-010-0120-1

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…It has been noted that the repeated-batch culture is affected by operating factors. In this view, it has been observed that the repeated-batch culture is influenced by harvesting times and harvesting volumes of the culture broth (Jin et al, 2011, Masuda et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Dashti

Abdeshahian

2016

Saudi Journal of Biological Sciences

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This research was performed based on a comparative study on fungal lipid production by a locally isolated strain Cunninghamella bainieri 2A1 in batch culture and repeated-batch culture using a nitrogen-limited medium. Lipid production in the batch culture was conducted to study the effect of different agitation rates on the simultaneous consumption of ammonium tartrate and glucose sources. Lipid production in the repeated-batch culture was studied by considering the effect of harvesting time and harvesting volume of the culture broth on the lipid accumulation. The batch cultivation was carried out in a 500 ml Erlenmeyer flask containing 200 ml of the fresh nitrogen-limited medium. Microbial culture was incubated at 30 °C under different agitation rates of 120, 180 and 250 rpm for 120 h. The repeated-batch culture was performed at three harvesting times of 12, 24 and 48 h using four harvesting cultures of 60%, 70%, 80% and 90%. Experimental results revealed that nitrogen source (ammonium tartrate) was fully utilized by C. bainieri 2A1 within 24 h in all agitation rates tested. It was also observed that a high amount of glucose in culture medium was consumed by C. bainieri 2A1 at 250 rpm agitation speed during the batch fermentation. Similar results showed that the highest lipid concentration of 2.96 g/L was obtained at an agitation rate of 250 rpm at 120 h cultivation time with the maximum lipid productivity of 7.0 × 10−2 mg/ml/h. On the other hand, experimental results showed that the highest lipid concentration produced in the repeated-batch culture was 3.30 g/L at the first cycle of 48 h harvesting time using 70% harvesting volume, while 0.23 g/L gamma-linolenic acid (GLA) was produced at the last cycle of 48 h harvesting time using 80% harvesting volume.

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Section: Introductionmentioning

confidence: 99%

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Dashti

Abdeshahian

2016

Saudi Journal of Biological Sciences

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“…Two statistical methods also indicated that potassium phosphate was the most significant factor for cell growth and production of CMCase. Potassium phosphate is one of the major salts in the medium for productions of microbial polysaccharides and enzymes as well as a well-known ingredient in buffer solutions [11,16,19]. Sodium chloride was reported to be used as a physiological modulator of biosynthetic pathway of biopolymers [10].…”

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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“…Potassium phosphate is one of major salts in the medium for productions of microbial polysaccharides and enzymes as well as a well-known ingredient in buffer solutions [13,23]. Sodium chloride was reported to be used as a physiological modulator of biosynthetic pathway of biopolymers [12,24].…”

Section: Optimization Of Salts In Medium For Production Of Cellobiasementioning

confidence: 99%

Enhanced Production of Cellobiase by a Marine Bacterium, Cellulophaga lytica LBH-14, in Pilot-Scaled Bioreactor Using Rice Bran

Cao¹,

Kim²,

Li³

et al. 2013

Journal of Life Science

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The aim of this work was to establish the optimal conditions for the production of cellobiase by a marine bacterium, Cellulophaga lytica LBH-14, using response-surface methodology (RSM). The optimal conditions of rice bran, ammonium chloride, and the initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for the production of cellobiase were 91.1 g/l, 9.02 g/l, and 6.6, respectively. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for the production of cellobiase were 4.46, 0.36, 0.27, and 0.73 g/l, respectively. The optimal temperatures for cell growth and for the production of cellobiase by C. lytica LBH-14 were 35 and 25℃, respectively. The maximal production of cellobiase in a 100 L bioreactor under optimized conditions in this study was 92.3 U/ml, which was 5.4 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of cellobiase by C. lytica LBH-14. The time for the production of cellobiase by the marine bacterium with submerged fermentations was reduced from 7 to 3 days, which resulted in enhanced productivity of cellobiase and a decrease in its production cost. This study found that the optimal conditions for the production of cellobiase were different from those of CMCase by C. lytica LBH-14.

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Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in repeated batch culture with optimized substitution of culture medium

Cited by 10 publications

References 29 publications

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Enhanced Production of Cellobiase by a Marine Bacterium, Cellulophaga lytica LBH-14, in Pilot-Scaled Bioreactor Using Rice Bran

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